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Characterization of tumor‑associated MUC1 and its glycans expressed in mucoepidermoid carcinoma

  • Authors:
    • Eisaku Isaka
    • Takanori Sugiura
    • Kazuhiko Hashimoto
    • Kazutaka Kikuta
    • Ukei Anazawa
    • Takeshi Nomura
    • Akihiko Kameyama
  • View Affiliations / Copyright

    Affiliations: Department of Oral Oncology, Oral and Maxillofacial Surgery, Ichikawa General Hospital, Tokyo Dental College, Ichikawa‑shi, Chiba 272‑8513, Japan, Department of Pathology and Laboratory Medicine, Ichikawa General Hospital, Tokyo Dental College, Ichikawa‑shi, Chiba 272‑8513, Japan, Department of Musculoskeletal Oncology and Orthopaedic Surgery, Tochigi Cancer Center, Utsunomiya, Tochigi 320‑0834, Japan, Department of Orthopaedic Surgery, Tokyo Dental College, Ichikawa‑shi, Chiba 272‑8513, Japan, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305‑8565, Japan
    Copyright: © Isaka et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 702
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    Published online on: August 3, 2021
       https://doi.org/10.3892/ol.2021.12963
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Abstract

Mucoepidermoid carcinoma (MEC) is one of the most frequently misdiagnosed tumors. Glycans are modulated by malignant transformation. Mucin 1 (MUC1) is a mucin whose expression is upregulated in various tumors, including MEC, and it has previously been investigated as a diagnostic and prognostic tumor marker. The present study aimed to reveal the differences in the mucin glycans between MEC and normal salivary glands (NSGs) to discover novel diagnostic markers. Soluble fractions of salivary gland homogenate prepared from three MEC salivary glands and 7 NSGs were evaluated. Mucins in MEC and NSGs were separated using supported molecular matrix electrophoresis, and stained with Alcian blue and monoclonal antibodies. The glycans of the separated mucins were analyzed by mass spectrometry. MUC1 was found in MEC but not in NSGs, and almost all glycans of MUC1 in MEC were sialylated, whereas the glycans of mucins in NSGs were less sialylated. The core 2 type glycans, (Hex)2(HexNAc)2(NeuAc)1 and (Hex)2(HexNAc)2(NeuAc)2, were found to be significantly abundant glycans of MUC1 in MEC. MEC markedly produced MUC1 modified with sialylated core 2 glycans. These data were obtained from the soluble fractions of salivary gland homogenates. These findings provide a basis for the utilization of MUC1 as a serum diagnostic marker for the preoperative diagnosis of MEC.
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Copy and paste a formatted citation
Spandidos Publications style
Isaka E, Sugiura T, Hashimoto K, Kikuta K, Anazawa U, Nomura T and Kameyama A: Characterization of tumor‑associated MUC1 and its glycans expressed in mucoepidermoid carcinoma. Oncol Lett 22: 702, 2021.
APA
Isaka, E., Sugiura, T., Hashimoto, K., Kikuta, K., Anazawa, U., Nomura, T., & Kameyama, A. (2021). Characterization of tumor‑associated MUC1 and its glycans expressed in mucoepidermoid carcinoma. Oncology Letters, 22, 702. https://doi.org/10.3892/ol.2021.12963
MLA
Isaka, E., Sugiura, T., Hashimoto, K., Kikuta, K., Anazawa, U., Nomura, T., Kameyama, A."Characterization of tumor‑associated MUC1 and its glycans expressed in mucoepidermoid carcinoma". Oncology Letters 22.4 (2021): 702.
Chicago
Isaka, E., Sugiura, T., Hashimoto, K., Kikuta, K., Anazawa, U., Nomura, T., Kameyama, A."Characterization of tumor‑associated MUC1 and its glycans expressed in mucoepidermoid carcinoma". Oncology Letters 22, no. 4 (2021): 702. https://doi.org/10.3892/ol.2021.12963
Copy and paste a formatted citation
x
Spandidos Publications style
Isaka E, Sugiura T, Hashimoto K, Kikuta K, Anazawa U, Nomura T and Kameyama A: Characterization of tumor‑associated MUC1 and its glycans expressed in mucoepidermoid carcinoma. Oncol Lett 22: 702, 2021.
APA
Isaka, E., Sugiura, T., Hashimoto, K., Kikuta, K., Anazawa, U., Nomura, T., & Kameyama, A. (2021). Characterization of tumor‑associated MUC1 and its glycans expressed in mucoepidermoid carcinoma. Oncology Letters, 22, 702. https://doi.org/10.3892/ol.2021.12963
MLA
Isaka, E., Sugiura, T., Hashimoto, K., Kikuta, K., Anazawa, U., Nomura, T., Kameyama, A."Characterization of tumor‑associated MUC1 and its glycans expressed in mucoepidermoid carcinoma". Oncology Letters 22.4 (2021): 702.
Chicago
Isaka, E., Sugiura, T., Hashimoto, K., Kikuta, K., Anazawa, U., Nomura, T., Kameyama, A."Characterization of tumor‑associated MUC1 and its glycans expressed in mucoepidermoid carcinoma". Oncology Letters 22, no. 4 (2021): 702. https://doi.org/10.3892/ol.2021.12963
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