GATA6‑AS1 inhibits ovarian cancer cell proliferation and migratory and invasive abilities by sponging miR‑19a‑5p and upregulating TET2
- Hua Xu
- Xiao Wang
- Yinghong Zhang
- Wei Zheng
- Huijie Zhang
Affiliations: Department of Obstetrics, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Gynecology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China
- Published online on: August 9, 2021 https://doi.org/10.3892/ol.2021.12979
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et al. This is an open access article distributed under the
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GATA6 antisense RNA 1 (GATA6‑AS1) has been reported to be involved in the progression of several types of cancer. In the present study, the role of GATA6‑AS1 in ovarian cancer (OC) was explored. Reverse transcription quantitative PCR was used to detect the expression of GATA6‑AS1, microRNA (miR)‑19a‑5p and tet methylcytosine dioxygenase 2 (TET2) in OC and adjacent normal tissues. Furthermore, OC cells with GATA‑AS1 either knocked down or overexpressed were established. The Cell Counting Kit‑8 assay was used to evaluate cell proliferation and a Transwell assay was used to assess the migratory and invasive abilities of OC cells. A dual luciferase reporter gene assay was used to determine whether GATA6‑AS1 and miR‑19a‑5p, and miR‑19a‑5p and TET2, may interact with each other. The results demonstrated that GATA6‑AS1 expression level was decreased in OC tissues and cells compared with control groups. In addition, GATA6‑AS1 overexpression significantly inhibited the proliferation and migratory and invasive abilities of OC cells, whereas GATA6‑AS1 downregulation had the opposite effects. Furthermore, GATA6‑AS1 adsorbed miR‑19a‑5p to repress its expression and GTA6‑AS1 indirectly upregulated TET2 expression. Taken together, the findings from this study suggested that GATA6‑AS1 could inhibit the proliferation and migratory and invasive abilities of OC cells via regulation of the miR‑19a‑5p/TET2 axis.