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Circular RNA SLC26A4 regulates the maturation of microRNA‑15a in non‑small cell lung cancer cells

  • Authors:
    • Qiankun Chen
    • Hua Li
    • Ji Liu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, Shanghai 200433, P.R. China, Department of Anesthesiology, Shanghai Pulmonary Hospital, Shanghai 200433, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 722
    |
    Published online on: August 10, 2021
       https://doi.org/10.3892/ol.2021.12983
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Abstract

To the best of our knowledge, the oncogenic role of circular RNA solute carrier family 26 member 4 (circSLC26A4) has only been reported in cervical cancer, while its role in non‑small cell lung cancer (NSCLC) is unknown. The present study explored the involvement of circSLC26A4 in NSCLC. NSCLC tissues and paired adjacent non‑tumor tissues were collected from 64 patients with NSCLC. The expression levels of circSLC26A4, mature microRNA‑15a (miR‑15a) and miR‑15a precursor in these tissues were determined by reverse transcription‑quantitative PCR (RT‑qPCR). NSCLC cells were transfected with pcDNA3.1‑circSLC26A4 vector to overexpress circSLC26A4, followed by the measurement of the expression levels of mature miR‑15a and miR‑15a precursor using RT‑qPCR. Cell proliferation was analyzed using a Cell Counting Kit‑8 assay. circSLC26A4 expression was upregulated in NSCLC tissues, and its high expression was significantly associated with poor survival of patients with NSCLC. The expression levels of circSLC26A4 were correlated with the expression levels of mature miR‑15a, but not the expression levels of miR‑15a precursor in NSCLC tissues. In NSCLC cells, overexpression of circSLC26A4 was associated with downregulation of mature miR‑15a expression, but not miR‑15a precursor expression. A cell proliferation assay revealed that overexpression of circSLC26A4 reduced the inhibitory effects of overexpression of miR‑15a on cell proliferation. Therefore, circSLC26A4 may suppress the maturation of miR‑15a in NSCLC to inhibit cancer cell proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Q, Li H and Liu J: Circular RNA SLC26A4 regulates the maturation of microRNA‑15a in non‑small cell lung cancer cells. Oncol Lett 22: 722, 2021.
APA
Chen, Q., Li, H., & Liu, J. (2021). Circular RNA SLC26A4 regulates the maturation of microRNA‑15a in non‑small cell lung cancer cells. Oncology Letters, 22, 722. https://doi.org/10.3892/ol.2021.12983
MLA
Chen, Q., Li, H., Liu, J."Circular RNA SLC26A4 regulates the maturation of microRNA‑15a in non‑small cell lung cancer cells". Oncology Letters 22.4 (2021): 722.
Chicago
Chen, Q., Li, H., Liu, J."Circular RNA SLC26A4 regulates the maturation of microRNA‑15a in non‑small cell lung cancer cells". Oncology Letters 22, no. 4 (2021): 722. https://doi.org/10.3892/ol.2021.12983
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Q, Li H and Liu J: Circular RNA SLC26A4 regulates the maturation of microRNA‑15a in non‑small cell lung cancer cells. Oncol Lett 22: 722, 2021.
APA
Chen, Q., Li, H., & Liu, J. (2021). Circular RNA SLC26A4 regulates the maturation of microRNA‑15a in non‑small cell lung cancer cells. Oncology Letters, 22, 722. https://doi.org/10.3892/ol.2021.12983
MLA
Chen, Q., Li, H., Liu, J."Circular RNA SLC26A4 regulates the maturation of microRNA‑15a in non‑small cell lung cancer cells". Oncology Letters 22.4 (2021): 722.
Chicago
Chen, Q., Li, H., Liu, J."Circular RNA SLC26A4 regulates the maturation of microRNA‑15a in non‑small cell lung cancer cells". Oncology Letters 22, no. 4 (2021): 722. https://doi.org/10.3892/ol.2021.12983
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