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Screening and identification of differentially expressed microRNAs in diffuse large B‑cell lymphoma based on microRNA microarray

  • Authors:
    • Hai-Xia Gao
    • Si-Jing Li
    • Meng-Bo Wang
    • Shu-Fang Yan
    • Wen-Li Cui
    • Zhi-Ping Ma
    • Jing Xue
    • Wei Sang
    • Wei Zhang
    • Xin-Xia Li
  • View Affiliations / Copyright

    Affiliations: Department of Pathology and NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region 832002, P.R. China, Department of Pathology, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang Uygur Autonomous Region 830054, P.R. China, Department of Ultrasound, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region 832002, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 753
    |
    Published online on: August 27, 2021
       https://doi.org/10.3892/ol.2021.13014
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Abstract

Diffuse large B‑cell lymphoma (DLBCL) is the most common type of B‑cell non‑Hodgkin lymphoma in adults and the pathogenesis of DLBCL is multifactorial and complex. Understanding the molecular mechanisms involved in DLBCL is important to identify new therapeutic targets. The present study aimed to screen and identify differentially expressed microRNAs (miRNAs/miRs) between diffuse large B‑cell lymphoma (DLBCL) and control [lymph node reactive hyperplasia (LRH)] groups, and to investigate whether miRNAs associated with DLBCL could serve as potential therapeutic targets. In total, 5 DLBCL experimental samples and 5 control samples were obtained from fresh patient tissues. Firstly, the fresh samples were analyzed using miRNA microarray to identify differentially expressed miRNAs. Next, three databases (TargetScan, microRNA.org and PITA) were used to predict by intersection the potential target genes of the 204 differential miRNAs identified, and a Venn diagram of the results was performed. Subsequently, the target genes of differential miRNAs were analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. Finally, to validate the miRNA microarray data, reverse transcription‑quantitative PCR (RT‑qPCR) was performed for 8 differentially expressed miRNAs (miR‑193a‑3p, miR‑19a‑3p, miR‑19b‑3p, miR‑370‑3p, miR‑1275, miR‑490‑5p, miR‑630 and miR‑665) using DLBCL and LRH fresh samples. In total, 204 miRNAs exhibited differential expression, including 105 downregulated and 54 upregulated miRNAs. The cut‑off criteria were set as P≤0.05 and fold‑change ≥2. A total of 7,522 potential target genes for the 204 miRNAs were predicted. Potential target genes were enriched in the following pathways: ‘Cancer’, ‘MAPK signaling pathway’, ‘regulation of actin cytoskeleton’, ‘focal adhesion’, ‘endocytosis’, ‘Wnt signaling pathway’, ‘axon guidance’, ‘calcium signaling pathway’ and ‘PI3K/AKT signaling pathway’. A total of 8 miRNAs were validated by RT‑qPCR, and 4 miRNAs (miR‑19b‑3p, miR‑193a‑3p, miR‑370‑3p and miR‑490‑5p) exhibited low expression levels in DLBCL (P<0.05), while miR‑630 was highly expressed in DLBCL (P<0.05). Overall, the present study screened 204 differentially expressed miRNAs and analyzed the expression levels of 8 differentially expressed miRNAs in DLBCL. These differentially expressed miRNAs may serve as therapeutic targets for improvement of therapeutic efficacy in DLBCL in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Gao H, Li S, Wang M, Yan S, Cui W, Ma Z, Xue J, Sang W, Zhang W, Li X, Li X, et al: Screening and identification of differentially expressed microRNAs in diffuse large B‑cell lymphoma based on microRNA microarray. Oncol Lett 22: 753, 2021.
APA
Gao, H., Li, S., Wang, M., Yan, S., Cui, W., Ma, Z. ... Li, X. (2021). Screening and identification of differentially expressed microRNAs in diffuse large B‑cell lymphoma based on microRNA microarray. Oncology Letters, 22, 753. https://doi.org/10.3892/ol.2021.13014
MLA
Gao, H., Li, S., Wang, M., Yan, S., Cui, W., Ma, Z., Xue, J., Sang, W., Zhang, W., Li, X."Screening and identification of differentially expressed microRNAs in diffuse large B‑cell lymphoma based on microRNA microarray". Oncology Letters 22.5 (2021): 753.
Chicago
Gao, H., Li, S., Wang, M., Yan, S., Cui, W., Ma, Z., Xue, J., Sang, W., Zhang, W., Li, X."Screening and identification of differentially expressed microRNAs in diffuse large B‑cell lymphoma based on microRNA microarray". Oncology Letters 22, no. 5 (2021): 753. https://doi.org/10.3892/ol.2021.13014
Copy and paste a formatted citation
x
Spandidos Publications style
Gao H, Li S, Wang M, Yan S, Cui W, Ma Z, Xue J, Sang W, Zhang W, Li X, Li X, et al: Screening and identification of differentially expressed microRNAs in diffuse large B‑cell lymphoma based on microRNA microarray. Oncol Lett 22: 753, 2021.
APA
Gao, H., Li, S., Wang, M., Yan, S., Cui, W., Ma, Z. ... Li, X. (2021). Screening and identification of differentially expressed microRNAs in diffuse large B‑cell lymphoma based on microRNA microarray. Oncology Letters, 22, 753. https://doi.org/10.3892/ol.2021.13014
MLA
Gao, H., Li, S., Wang, M., Yan, S., Cui, W., Ma, Z., Xue, J., Sang, W., Zhang, W., Li, X."Screening and identification of differentially expressed microRNAs in diffuse large B‑cell lymphoma based on microRNA microarray". Oncology Letters 22.5 (2021): 753.
Chicago
Gao, H., Li, S., Wang, M., Yan, S., Cui, W., Ma, Z., Xue, J., Sang, W., Zhang, W., Li, X."Screening and identification of differentially expressed microRNAs in diffuse large B‑cell lymphoma based on microRNA microarray". Oncology Letters 22, no. 5 (2021): 753. https://doi.org/10.3892/ol.2021.13014
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