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Article Open Access

Role of NELF‑B in supporting epithelial‑mesenchymal transition and cell proliferation during hepatocellular carcinoma progression

  • Authors:
    • Mennatallah Hani Ghouraba
    • Razan Jamil Masad
    • Eric Zadok Mpingirika
    • Omnia Mahmoud Abdelraheem
    • Rached Zeghlache
    • Aya M Alserw
    • Asma Amleh
  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology, School of Sciences and Engineering, The American University in Cairo, New Cairo 11835, Egypt, Department of Biology, School of Sciences and Engineering, The American University in Cairo, New Cairo 11835, Egypt
    Copyright: © Ghouraba et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 761
    |
    Published online on: September 6, 2021
       https://doi.org/10.3892/ol.2021.13022
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Abstract

Negative elongation factor‑B (NELF‑B), also known as cofactor of BRCA1 (COBRA1), is one of the four subunits of the NELF complex. It interacts with BRCA1, in addition to other transcription complexes in various tissues. The NELF complex represses the transcription of several genes by stalling RNA polymerase II during the early phase of transcription elongation. The role of NELF‑B in liver cancer and hepatocellular carcinoma (HCC), the most prevalent type of liver cancer, remains to be elucidated. It has been previously demonstrated that silencing of NELF‑B inhibits the proliferation and migration of HepG2 cells. The present study aimed to investigate the consequences of ectopic expression and silencing of NELF‑B in liver cancer HepG2 and SNU449 cell lines. Functional assays were performed to examine the effects on gene and protein expression, viability, migration and invasion of cells. Overexpression of NELF‑B did not alter the proliferation and migration of HepG2 cells, or the expression of tested genes, indicating that overexpression alone may not be sufficient for altering these features in HepG2 cells. By contrast, knockdown of NELF‑B in SNU449 cells resulted in decreased cell proliferation, together with induction of apoptosis and decreased expression levels of Ki‑67 and survivin, which are markers of proliferation and inhibition of apoptosis, respectively. Additionally, silencing of NELF‑B resulted in a significant decrease in the hallmarks of epithelial‑mesenchymal transition (EMT), including cell migration and invasion, and decreased the expression levels of EMT markers, such as N‑cadherin, vimentin and β‑catenin. Decreased expression levels of forkhead box F2 transcription factor and increased mRNA levels of trefoil factor 1, a putative tumor suppressor, were also detected following the silencing of NELF‑B. The current results demonstrated that NELF‑B enhanced the manifestation of most hallmarks of cancer, including cell proliferation, migration, invasion and inhibition of apoptosis, indicating its critical role in the progression of HCC.
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1 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Simonetti RG, Liberati A, Angiolini C and Pagliaro L: Treatment of hepatocellular carcinoma: A systematic review of randomized controlled trials. Ann Oncol. 8:117–136. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Chidambaranathan-Reghupaty S, Fisher PB and Sarkar D: Hepatocellular carcinoma (HCC): Epidemiology, etiology and molecular classification. Adv Cancer Res. 149:1–61. 2021. View Article : Google Scholar : PubMed/NCBI

4 

Zhao YJ, Ju Q and Li GC: Tumor markers for hepatocellular carcinoma. Mol Clin Oncol. 1:593–598. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Sanyal AJ, Yoon SK and Lencioni R: The etiology of hepatocellular carcinoma and consequences for treatment. Oncologist. 15 (Suppl 4):S14–S22. 2010. View Article : Google Scholar

6 

Dong Y, Wang WP, Mao F, Zhang Q, Yang D, Tannapfel A, Meloni MF, Neye H, Clevert DA and Dietrich CF: Imaging features of fibrolamellar hepatocellular carcinoma with contrast-enhanced ultrasound. Ultraschall Med. 42:306–313. 2021. View Article : Google Scholar : PubMed/NCBI

7 

Jiang HY, Chen J, Xia CC, Cao LK, Duan T and Song B: Noninvasive imaging of hepatocellular carcinoma: From diagnosis to prognosis. World J Gastroenterol. 24:2348–2362. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Andreana L, Isgrò G, Pleguezuelo M, Germani G and Burroughs AK: Surveillance and diagnosis of hepatocellular carcinoma in patients with cirrhosis. World J Hepatol. 1:48–61. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Janevska D, Chaloska-Ivanova V and Janevski V: Hepatocellular carcinoma: Risk factors, diagnosis and treatment. Open Access Maced J Med Sci. 3:732–736. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Dhanasekaran R, Bandoh S and Roberts LR: Molecular pathogenesis of hepatocellular carcinoma and impact of therapeutic advances. F1000Res. 5:F1000 Faculty Rev. –879. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Ye Q, Hu YF, Zhong H, Nye AC, Belmont AS and Li R: BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. J Cell Biol. 155:911–921. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, et al: Human transcription elongation factor NELF: Identification of novel subunits and reconstitution of the functionally active complex. Mol Cell Biol. 23:1863–1873. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, et al: DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs. Genes Dev. 12:343–356. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Yankulov K, Yamashita K, Roy R, Egly JM and Bentley DL: The transcriptional elongation inhibitor 5,6-Dichloro-1-β-D-ribofuranosylbenzimidazole inhibits transcription Factor IIH-associated protein kinase. J Biol Chem. 270:23922–23925. 1995. View Article : Google Scholar : PubMed/NCBI

15 

Yamaguchi Y, Takagi T, Wada T, Yano K, Furuya A, Sugimoto S, Hasegawa J and Handa H: NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation. Cell. 97:41–51. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Yamaguchi Y, Filipovska J, Yano K, Furuya A, Inukai N, Narita T, Wada T, Sugimoto S, Konarska MM and Handa H: Stimulation of RNA polymerase II elongation by hepatitis delta antigen. Science. 293:124–127. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Sun J, Watkins G, Blair AL, Moskaluk C, Ghosh S, Jiang WG and Li R: Deregulation of cofactor of BRCA1 expression in breast cancer cells. J Cell Biochem. 103:1798–1807. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Sun J, Blair AL, Aiyar SE and Li R: Cofactor of BRCA1 modulates androgen-dependent transcription and alternative splicing. J Steroid Biochem Mol Biol. 107:131–139. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Zhong H, Zhu J, Zhang H, Ding L, Sun Y, Huang C and Ye Q: COBRA1 inhibits AP-1 transcriptional activity in transfected cells. Biochem Biophys Res Commun. 325:568–573. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Aiyar SE, Sun JL, Blair AL, Moskaluk CA, Lu YZ, Ye QN, Yamaguchi Y, Mukherjee A, Ren DM, Handa H and Li R: Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor. Genes Dev. 18:2134–2146. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Aiyar SE, Cho H, Lee J and Li R: Concerted transcriptional regulation by BRCA1 and COBRA1 in breast cancer cells. Int J Biol Sci. 3:486–492. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Aiyar SE, Blair AL, Hopkinson DA, Bekiranov S and Li R: Regulation of clustered gene expression by cofactor of BRCA1 (COBRA1) in breast cancer cells. Oncogene. 26:2543–2553. 2007. View Article : Google Scholar : PubMed/NCBI

23 

McChesney PA, Aiyar SE, Lee OJ, Zaika A, Moskaluk C, Li R and El-Rifai W: Cofactor of BRCA1: A novel transcription factor regulator in upper gastrointestinal adenocarcinomas. Cancer Res. 66:1346–1353. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Yun H, Bedolla R, Horning A, Li R, Chiang HC, Huang TH, Reddick R, Olumi AF, Ghosh R and Kumar AP: BRCA1 interacting protein COBRA1 facilitates adaptation to Castrate-Resistant growth conditions. Int J Mol Sci. 19:21042018. View Article : Google Scholar : PubMed/NCBI

25 

El Zeneini E, Kamel S, El-Meteini M and Amleh A: Knockdown of COBRA1 decreases the proliferation and migration of hepatocellular carcinoma cells. Oncol Rep. 37:1896–1906. 2017. View Article : Google Scholar : PubMed/NCBI

26 

López-Terrada D, Cheung SW, Finegold MJ and Knowles BB: Hep G2 is a hepatoblastoma-derived cell line. Hum Pathol. 40:1512–1515. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Strober W: Trypan blue exclusion test of cell viability. Curr Protoc Immunol. doi: 10.1002/0471142735.ima03bs21.

28 

Schneider CA, Rasband WS and Eliceiri KW: NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 9:671–675. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Jonkman JE, Cathcart JA, Xu F, Bartolini ME, Amon JE, Stevens KM and Colarusso P: An introduction to the wound healing assay using live-cell microscopy. Cell Adh Migr. 8:440–451. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Gebäck T, Schulz MMP, Koumoutsakos P and Detmar M: TScratch: A novel and simple software tool for automated analysis of monolayer wound healing assays. BioTechniques. 46:265–274. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Boleman AI, Tănasie G, Găluşcan A, Cristea MI, Bojin FM, Panaitescu C and Păunescu V: Studies regarding the in vitro wound healing potential of mouse dental pulp stem-like progenitor cells. Biotechnol & Biotec Eq. 26:2781–2785. 2012. View Article : Google Scholar

33 

Kalluri R and Weinberg RA: The basics of epithelial-mesenchymal transition. J Clin Invest. 119:1420–1428. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Cho SB, Lee KH, Lee JH, Park SY, Lee WS, Park CH, Kim HS, Choi SK and Rew JS: Expression of E- and N-cadherin and clinicopathology in hepatocellular carcinoma. Pathol Int. 58:635–642. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Dahmani R, Just PA and Perret C: The Wnt/β-catenin pathway as a therapeutic target in human hepatocellular carcinoma. Clin Res Hepatol Gastroenterol. 35:709–713. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Khramtsov AI, Khramtsova GF, Tretiakova M, Huo D, Olopade OI and Goss KH: Wnt/β-catenin pathway activation is enriched in basal-like breast cancers and predicts poor outcome. Am J Pathol. 176:2911–2920. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Kobayashi M, Honma T, Matsuda Y, Suzuki Y, Narisawa R, Ajioka Y and Asakura H: Nuclear translocation of β-catenin in colorectal cancer. Br J Cancer. 82:1689–1693. 2000.PubMed/NCBI

38 

Dou C, Jin X, Sun L, Zhang B, Han M and Li T: FOXF2 deficiency promotes hepatocellular carcinoma metastasis by inducing mesenchymal-epithelial transition. Cancer Biomark. 19:447–454. 2017. View Article : Google Scholar : PubMed/NCBI

39 

Fukuda S and Pelus LM: Survivin, a cancer target with an emerging role in normal adult tissues. Mol Cancer Ther. 5:1087–1098. 2006. View Article : Google Scholar : PubMed/NCBI

40 

Kerr JF, Wyllie AH and Currie AR: Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 26:239–257. 1972. View Article : Google Scholar : PubMed/NCBI

41 

Lacroix M: Significance, detection and markers of disseminated breast cancer cells. Endocr Relat Cancer. 13:1033–1067. 2006. View Article : Google Scholar : PubMed/NCBI

42 

Im S, Yoo C, Jung JH, Choi HJ, Yoo J and Kang CS: Reduced expression of TFF1 and increased expression of TFF3 in gastric cancer: Correlation with clinicopathological parameters and prognosis. Int J Med Sci. 10:133–140. 2013. View Article : Google Scholar : PubMed/NCBI

43 

Vestergaard EM, Borre M, Poulsen SS, Nexø E and Tørring N: Plasma levels of trefoil factors are increased in patients with advanced prostate cancer. Clin Cancer Res. 12((3 Pt 1)): 807–812. 2006. View Article : Google Scholar : PubMed/NCBI

44 

Yusufu A, Shayimu P, Tuerdi R, Fang C, Wang F and Wang H: TFF3 and TFF1 expression levels are elevated in colorectal cancer and promote the malignant behavior of colon cancer by activating the EMT process. Int J Oncol. 55:789–804. 2019.PubMed/NCBI

45 

Casado E, Garcia VM, Sánchez JJ, Gómez Del Pulgar MT, Feliu J, Maurel J, Castelo B, Moreno Rubio J, López RA, García-Cabezas MÁ, et al: Upregulation of trefoil factor 3 (TFF3) after rectal cancer chemoradiotherapy is an adverse prognostic factor and a potential therapeutic target. Int J Radiat Oncol Biol Phys. 84:1151–1158. 2012. View Article : Google Scholar : PubMed/NCBI

46 

Ochiai Y, Yamaguchi J, Kokuryo T, Yokoyama Y, Ebata T and Nagino M: Trefoil factor family 1 inhibits the development of hepatocellular carcinoma by regulating β-catenin activation. Hepatology. 72:503–517. 2020. View Article : Google Scholar : PubMed/NCBI

47 

You ML, Chen YJ, Chong QY, Wu MM, Pandey V, Chen RM, Liu L, Ma L, Wu ZS, Zhu T and Lobie PE: Trefoil factor 3 mediation of oncogenicity and chemoresistance in hepatocellular carcinoma is AKT-BCL-2 dependent. Oncotarget. 8:39323–39344. 2017. View Article : Google Scholar : PubMed/NCBI

48 

Youssef A, Shawer H, Afify A and Amleh A: The potential involvement of the cofactor of BRCA1 in hepatocellular carcinoma pathogenesis. Adv Mod Oncol Res. 2:2242016. View Article : Google Scholar : PubMed/NCBI

49 

Hanahan D and Weinberg RA: The hallmarks of cancer. Cell. 100:57–70. 2000. View Article : Google Scholar : PubMed/NCBI

50 

Sun J and Li R: Human negative elongation factor activates transcription and regulates alternative transcription initiation. J Biol Chem. 285:6443–6452. 2010. View Article : Google Scholar : PubMed/NCBI

51 

Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD and Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: A very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 123:513–522. 1993. View Article : Google Scholar : PubMed/NCBI

52 

Hunter AM, LaCasse EC and Korneluk RG: The inhibitors of apoptosis (IAPs) as cancer targets. Apoptosis. 12:1543–1568. 2007. View Article : Google Scholar : PubMed/NCBI

53 

Ambrosini G, Adida C and Altieri DC: A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med. 3:917–921. 1997. View Article : Google Scholar : PubMed/NCBI

54 

Han G, Gong H, Wang Y, Guo S and Liu K: AMPK/mTOR-mediated inhibition of survivin partly contributes to metformin-induced apoptosis in human gastric cancer cell. Cancer Biol Ther. 16:77–87. 2015. View Article : Google Scholar : PubMed/NCBI

55 

Su C: Survivin in survival of hepatocellular carcinoma. Cancer Lett. 379:184–190. 2016. View Article : Google Scholar : PubMed/NCBI

56 

Giodini A, Kallio MJ, Wall NR, Gorbsky GJ, Tognin S, Marchisio PC, Symons M and Altieri DC: Regulation of microtubule stability and mitotic progression by survivin. Cancer Res. 62:2462–2467. 2002.PubMed/NCBI

57 

Suzuki A, Hayashida M, Ito T, Kawano H, Nakano T, Miura M, Akahane K and Shiraki K: Survivin initiates cell cycle entry by the competitive interaction with Cdk4/p16(INK4a) and Cdk2/cyclin E complex activation. Oncogene. 19:3225–3234. 2000. View Article : Google Scholar : PubMed/NCBI

58 

O'Connor DS, Schechner JS, Adida C, Mesri M, Rothermel AL, Li F, Nath AK, Pober JS and Altieri DC: Control of apoptosis during angiogenesis by survivin expression in endothelial cells. Am J Pathol. 156:393–398. 2000. View Article : Google Scholar : PubMed/NCBI

59 

Kramer A, Liashkovich I, Oberleithner H, Ludwig S, Mazur I and Shahin V: Apoptosis leads to a degradation of vital components of active nuclear transport and a dissociation of the nuclear lamina. Proc Natl Acad Sci USA. 105:11236–11241. 2008. View Article : Google Scholar : PubMed/NCBI

60 

Goldman RD, Khuon S, Chou YH, Opal P and Steinert PM: The function of intermediate filaments in cell shape and cytoskeletal integrity. J Cell Biol. 134:971–983. 1996. View Article : Google Scholar : PubMed/NCBI

61 

Mrozik KM, Blaschuk OW, Cheong CM, Zannettino ACW and Vandyke K: N-cadherin in cancer metastasis, its emerging role in haematological malignancies and potential as a therapeutic target in cancer. BMC Cancer. 18:9392018. View Article : Google Scholar : PubMed/NCBI

62 

Zhan DQ, Wei S, Liu C, Liang BY, Ji GB, Chen XP, Xiong M and Huang ZY: Reduced N-cadherin expression is associated with metastatic potential and poor surgical outcomes of hepatocellular carcinoma. J Gastroenterol Hepatol. 27:173–180. 2012. View Article : Google Scholar : PubMed/NCBI

63 

Zhou SJ, Liu FY, Zhang AH, Liang HF, Wang Y, Ma R, Jiang YH and Sun NF: MicroRNA-199b-5p attenuates TGF-beta1-induced epithelial-mesenchymal transition in hepatocellular carcinoma. Br J Cancer. 117:233–244. 2017. View Article : Google Scholar : PubMed/NCBI

64 

Noordermeer J, Klingensmith J, Perrimon N and Nusse R: Dishevelled and armadillo act in the Wingless signalling pathway in Drosophila. Nature. 367:80–83. 1994. View Article : Google Scholar : PubMed/NCBI

65 

Peifer M, Berg S and Reynolds AB: A repeating amino acid motif shared by proteins with diverse cellular roles. Cell. 76:789–791. 1994. View Article : Google Scholar : PubMed/NCBI

66 

Laurent-Puig P and Zucman-Rossi J: Genetics of hepatocellular tumors. Oncogene. 25:3778–3786. 2006. View Article : Google Scholar : PubMed/NCBI

67 

Papkoff J, Rubinfeld B, Schryver B and Polakis P: Wnt-1 regulates free pools of catenins and stabilizes APC-catenin complexes. Mol Cell Biol. 16:2128–2134. 1996. View Article : Google Scholar : PubMed/NCBI

68 

Behrens J, von Kries JP, Kühl M, Bruhn L, Wedlich D, Grosschedl R and Birchmeier W: Functional interaction of β-catenin with the transcription factor LEF-1. Nature. 382:638–642. 1996. View Article : Google Scholar : PubMed/NCBI

69 

Huber O, Korn R, McLaughlin J, Ohsugi M, Herrmann BG and Kemler R: Nuclear localization of β-catenin by interaction with transcription factor LEF-1. Mech Dev. 59:3–10. 1996. View Article : Google Scholar : PubMed/NCBI

70 

Molenaar M, van de Wetering M, Oosterwegel M, Peterson-Maduro J, Godsave S, Korinek V, Roose J, Destrée O and Clevers H: XTcf-3 transcription factor mediates β-catenin-induced axis formation in xenopus embryos. Cell. 86:391–399. 1996. View Article : Google Scholar : PubMed/NCBI

71 

Gilles C, Polette M, Mestdagt M, Nawrocki-Raby B, Ruggeri P, Birembaut P and Foidart JM: Transactivation of vimentin by beta-catenin in human breast cancer cells. Cancer Res. 63:2658–2664. 2003.PubMed/NCBI

72 

Lowy AM, Clements WM, Bishop J, Kong L, Bonney T, Sisco K, Aronow B, Fenoglio-Preiser C and Groden J: β-catenin/Wnt signaling regulates expression of the membrane type 3 matrix metalloproteinase in gastric cancer. Cancer Res. 66:4734–4741. 2006. View Article : Google Scholar : PubMed/NCBI

73 

Gradl D, Kühl M and Wedlich D: The Wnt/Wg signal transducer beta-catenin controls fibronectin expression. Mol Cell Biol. 19:5576–5587. 1999. View Article : Google Scholar : PubMed/NCBI

74 

Tapia JC, Torres VA, Rodriguez DA, Leyton L and Quest AF: Casein kinase 2 (CK2) increases survivin expression via enhanced beta-catenin-T cell factor/lymphoid enhancer binding factor-dependent transcription. Proc Natl Acad Sci USA. 103:15079–15084. 2006. View Article : Google Scholar : PubMed/NCBI

75 

He W, Kang Y, Zhu W, Zhou B, Jiang X, Ren C and Guo W: FOXF2 acts as a crucial molecule in tumours and embryonic development. Cell Death Dis. 11:4242020. View Article : Google Scholar : PubMed/NCBI

76 

Shao J, Cao J, Liu Y, Mei H, Zhang Y and Xu W: MicroRNA-519a promotes proliferation and inhibits apoptosis of hepatocellular carcinoma cells by targeting FOXF2. FEBS Open Bio. 5:893–899. 2015. View Article : Google Scholar : PubMed/NCBI

77 

Shi Z, Liu J, Yu X, Huang J, Shen S, Zhang Y, Han R, Ge N and Yang Y: Loss of FOXF2 expression predicts poor prognosis in hepatocellular carcinoma patients. Ann Surg Oncol. 23:211–217. 2016. View Article : Google Scholar : PubMed/NCBI

78 

Soutto M, Peng D, Katsha A, Chen Z, Piazuelo MB, Washington MK, Belkhiri A, Correa P and El-Rifai W: Activation of β-catenin signalling by TFF1 loss promotes cell proliferation and gastric tumorigenesis. Gut. 64:1028–1039. 2015. View Article : Google Scholar : PubMed/NCBI

79 

Dang H, Takai A, Forgues M, Pomyen Y, Mou H, Xue W, Ray D, Ha KCH, Morris QD, Hughes TR and Wang XW: Oncogenic activation of the RNA binding protein NELFE and MYC signaling in hepatocellular carcinoma. Cancer Cell. 32:101–114.e8. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ghouraba MH, Masad RJ, Mpingirika EZ, Abdelraheem OM, Zeghlache R, Alserw AM and Amleh A: Role of NELF‑B in supporting epithelial‑mesenchymal transition and cell proliferation during hepatocellular carcinoma progression. Oncol Lett 22: 761, 2021.
APA
Ghouraba, M.H., Masad, R.J., Mpingirika, E.Z., Abdelraheem, O.M., Zeghlache, R., Alserw, A.M., & Amleh, A. (2021). Role of NELF‑B in supporting epithelial‑mesenchymal transition and cell proliferation during hepatocellular carcinoma progression. Oncology Letters, 22, 761. https://doi.org/10.3892/ol.2021.13022
MLA
Ghouraba, M. H., Masad, R. J., Mpingirika, E. Z., Abdelraheem, O. M., Zeghlache, R., Alserw, A. M., Amleh, A."Role of NELF‑B in supporting epithelial‑mesenchymal transition and cell proliferation during hepatocellular carcinoma progression". Oncology Letters 22.5 (2021): 761.
Chicago
Ghouraba, M. H., Masad, R. J., Mpingirika, E. Z., Abdelraheem, O. M., Zeghlache, R., Alserw, A. M., Amleh, A."Role of NELF‑B in supporting epithelial‑mesenchymal transition and cell proliferation during hepatocellular carcinoma progression". Oncology Letters 22, no. 5 (2021): 761. https://doi.org/10.3892/ol.2021.13022
Copy and paste a formatted citation
x
Spandidos Publications style
Ghouraba MH, Masad RJ, Mpingirika EZ, Abdelraheem OM, Zeghlache R, Alserw AM and Amleh A: Role of NELF‑B in supporting epithelial‑mesenchymal transition and cell proliferation during hepatocellular carcinoma progression. Oncol Lett 22: 761, 2021.
APA
Ghouraba, M.H., Masad, R.J., Mpingirika, E.Z., Abdelraheem, O.M., Zeghlache, R., Alserw, A.M., & Amleh, A. (2021). Role of NELF‑B in supporting epithelial‑mesenchymal transition and cell proliferation during hepatocellular carcinoma progression. Oncology Letters, 22, 761. https://doi.org/10.3892/ol.2021.13022
MLA
Ghouraba, M. H., Masad, R. J., Mpingirika, E. Z., Abdelraheem, O. M., Zeghlache, R., Alserw, A. M., Amleh, A."Role of NELF‑B in supporting epithelial‑mesenchymal transition and cell proliferation during hepatocellular carcinoma progression". Oncology Letters 22.5 (2021): 761.
Chicago
Ghouraba, M. H., Masad, R. J., Mpingirika, E. Z., Abdelraheem, O. M., Zeghlache, R., Alserw, A. M., Amleh, A."Role of NELF‑B in supporting epithelial‑mesenchymal transition and cell proliferation during hepatocellular carcinoma progression". Oncology Letters 22, no. 5 (2021): 761. https://doi.org/10.3892/ol.2021.13022
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