Open Access

LAPTM4B promotes the progression of bladder cancer by stimulating cell proliferation and invasion

  • Authors:
    • Yanhua Yin
    • Yanyan Fan
    • Gang Yu
    • Ying Du
  • View Affiliations

  • Published online on: September 8, 2021     https://doi.org/10.3892/ol.2021.13026
  • Article Number: 765
  • Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Bladder cancer is a highly metastatic tumor and one of the most common malignant tumors originating in the urinary system. Due to the complicated etiology and lack of significant early symptoms, the diagnosis and treatment of bladder cancer is difficult. Lysosome‑associated transmembrane protein 4β (LAPTM4B) was reported to be involved in the development and progression of several types of tumor, however, its potential effect on the development and metastasis of bladder cancer is still unclear. Immunohistochemistry was performed to detect the protein expression level of LAPTM4B in bladder cancer tissues and short hairpin RNAs targeting LAPTM4B were transfected into bladder cancer cells to knockdown its expression. MTT and colony formation assays were performed to detect cell proliferation, while wound healing and Transwell invasion assays were performed to detect cell migration and invasion, respectively. In addition, tumor growth assays were performed to confirm the effects of LAPTM4B in mice. The present study demonstrated that LAPTM4B was associated with the prognosis of patients with bladder cancer. In addition, LAPTM4B was associated with clinical characteristics, including tumor stage and recurrence. The results further showed that LAPTM4B knockdown could suppress the proliferation of bladder cancer cell lines. In addition, the migration and invasion of T24 and 5637 cells was suppressed following LAPTM4B knockdown in vitro. The in vivo data confirmed that knockdown of LAPTM4B markedly inhibited tumor growth and metastasis in mice. In summary, the results from the present study provide strong evidence of the effects of LAPTM4B in bladder cancer progression.
View Figures
View References

Related Articles

Journal Cover

November-2021
Volume 22 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yin Y, Fan Y, Yu G and Du Y: LAPTM4B promotes the progression of bladder cancer by stimulating cell proliferation and invasion. Oncol Lett 22: 765, 2021
APA
Yin, Y., Fan, Y., Yu, G., & Du, Y. (2021). LAPTM4B promotes the progression of bladder cancer by stimulating cell proliferation and invasion. Oncology Letters, 22, 765. https://doi.org/10.3892/ol.2021.13026
MLA
Yin, Y., Fan, Y., Yu, G., Du, Y."LAPTM4B promotes the progression of bladder cancer by stimulating cell proliferation and invasion". Oncology Letters 22.5 (2021): 765.
Chicago
Yin, Y., Fan, Y., Yu, G., Du, Y."LAPTM4B promotes the progression of bladder cancer by stimulating cell proliferation and invasion". Oncology Letters 22, no. 5 (2021): 765. https://doi.org/10.3892/ol.2021.13026