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Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression

  • Authors:
    • Ming An
    • Xuefeng Xing
    • Tonghua Chen
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Sanya People's Hospital, Sanya, Hainan 572000, P.R. China
    Copyright: © An et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 772
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    Published online on: September 9, 2021
       https://doi.org/10.3892/ol.2021.13033
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Abstract

The long non‑coding RNA, urothelial cancer‑associated 1 (UCA1) is an important regulator in several tumors. However, to the best of our knowledge, the clinical roles of UCA1 in cervical cancer remain unclear. Thus, the present study aimed to investigate the function and mechanism of UCA1 in cervical cancer. Reverse transcription‑quantitative PCR analysis was performed to detect UCA1 and microRNA (miR)‑299‑3p expression in cervical cancer tissues and cell lines. The Cell Counting Kit‑8 and Transwell assays were performed to assess cell proliferation and invasion, respectively. Furthermore, the dual‑luciferase reporter assay was performed to confirm the association between UCA1 and miR‑299‑3p. Rescue experiments were performed to determine the mechanism of the UCA1/miR‑299‑3p axis. The results demonstrated that UCA1 expression was upregulated in cervical cancer tissues and cell lines. Furthermore, overexpression of UCA1 enhanced the proliferation and invasion of cervical cancer cells, the effects of which were reversed following UCA1 knockdown. Notably, UCA1 interacted with miR‑299‑3p and negatively regulated miR‑299‑3p expression. In addition, miR‑299‑3p expression was downregulated in cervical cancer tissues and cell lines. Overexpression of miR‑299‑3p suppressed the proliferation and invasion of cervical cancer cells, reversing the effects of UCA1 knockdown on cervical cancer cell proliferation. Taken together, the results of the present study suggest that UCA1 promotes cell proliferation and invasion by regulating miR‑299‑3p expression in cervical cancer.
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Copy and paste a formatted citation
Spandidos Publications style
An M, Xing X and Chen T: Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression. Oncol Lett 22: 772, 2021.
APA
An, M., Xing, X., & Chen, T. (2021). Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression. Oncology Letters, 22, 772. https://doi.org/10.3892/ol.2021.13033
MLA
An, M., Xing, X., Chen, T."Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression". Oncology Letters 22.5 (2021): 772.
Chicago
An, M., Xing, X., Chen, T."Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression". Oncology Letters 22, no. 5 (2021): 772. https://doi.org/10.3892/ol.2021.13033
Copy and paste a formatted citation
x
Spandidos Publications style
An M, Xing X and Chen T: Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression. Oncol Lett 22: 772, 2021.
APA
An, M., Xing, X., & Chen, T. (2021). Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression. Oncology Letters, 22, 772. https://doi.org/10.3892/ol.2021.13033
MLA
An, M., Xing, X., Chen, T."Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression". Oncology Letters 22.5 (2021): 772.
Chicago
An, M., Xing, X., Chen, T."Long non‑coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA‑299‑3p expression". Oncology Letters 22, no. 5 (2021): 772. https://doi.org/10.3892/ol.2021.13033
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