Open Access

Involvement of PBRM1 in VHL disease‑associated clear cell renal cell carcinoma and its putative relationship with the HIF pathway

  • Authors:
    • Sophie Gad
    • Gwenaël Le Teuff
    • Baptiste Nguyen
    • Virginie Verkarre
    • Veronique Duchatelle
    • Vincent Molinie
    • Katia Posseme
    • Benjamin Grandon
    • Melanie Da Costa
    • Bastien Job
    • Guillaume Meurice
    • Nathalie Droin
    • Arnaud Mejean
    • Sophie Couve
    • Flore Renaud
    • Betty Gardie
    • Bin Tean Teh
    • Stephane Richard
    • Sophie Ferlicot
  • View Affiliations

  • Published online on: October 15, 2021     https://doi.org/10.3892/ol.2021.13096
  • Article Number: 835
  • Copyright: © Gad et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Von Hippel‑Lindau (VHL) disease is the main cause of inherited clear‑cell renal cell carcinoma (ccRCC) and is caused by germline mutations in the VHL tumor suppressor gene. Bi‑allelic VHL alterations lead to inactivation of pVHL, which plays a major role by downstream activation of the hypoxia inducible factor (HIF) pathway. Somatic VHL mutations occur in 80% of sporadic ccRCC cases and the second most frequently mutated gene is polybromo 1 (PBRM1). As there is currently no data regarding PBRM1 involvement in VHL disease‑associated ccRCC, the aim of the present study was to assess the PBRM1 mutational status, and PBRM1 and HIF expression in VHL disease‑associated ccRCC series compared with a sporadic series. PBRM1 gene was screened by Sanger sequencing for 23 VHL‑disease‑associated ccRCC and 22 sporadic ccRCC cases. Immunohistochemical studies were performed to detect the expression of PBRM1, HIF1 and HIF2 for all cases. In VHL‑associated tumors, 13.0% (n=3/23) had PBRM1 somatic mutations and 17.4% (n=4/23) had a loss of PBRM1 nuclear expression. In sporadic cases, 27.3% (n=6/22) showed PBRM1 somatic mutations and 45.5% (n=10/22) had a loss of PBRM1 nuclear expression. Loss of PBRM1 was associated with an advanced tumor stage. HIF1‑positive tumors were observed more frequently in the VHL‑associated ccRCC than in the sporadic series. Furthermore, in the VHL cohort, PBRM1 expression appeared to be associated more with HIF1 than with HIF2. Given that hereditary tumors tend to be less aggressive, these results would suggest that co‑expression of PBRM1 and HIF1 may have a less oncogenic role in VHL‑associated ccRCC.
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