lncRNA GAS8‑AS1 regulates cancer cell proliferation and predicts poor survival of patients with gastric cancer

Li,Chao; Wang,Hui; Meng,Song; Hong,Jian; Yao,Libin; Shao,Yong; Zhu,Xiaocheng

doi:10.3892/ol.2021.13166

lncRNA GAS8‑AS1 regulates cancer cell proliferation and predicts poor survival of patients with gastric cancer

Authors:
- Chao Li
- Hui Wang
- Song Meng
- Jian Hong
- Libin Yao
- Yong Shao
- Xiaocheng Zhu
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Affiliations: Department of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221006, P.R. China
Published online on: December 14, 2021 https://doi.org/10.3892/ol.2021.13166
Article Number: 48

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Abstract

Long non‑coding (lnc)RNAs have been recognized as important regulators in gastric cancer. lncRNA GAS8‑AS1 is considered a tumor suppressor in multiple types of cancer, such as papillary thyroid carcinoma, ovarian cancer and colorectal cancer. However, the specific role of GAS8‑AS1 in gastric cancer remains to be fully elucidated. The aim of the present study was to investigate the role of GAS8‑AS1 in gastric cancer and its potential underlying mechanisms of action. The expression levels of GAS8‑AS1, microRNA (miR)‑21‑3p, PTEN and pyruvate dehydrogenase (E1) alpha subunit gene (PDHA1) in gastric cancer and non‑cancerous tissues, as well as in gastric cancer cell lines, were detected using reverse transcription‑quantitative PCR. Cell proliferation was detected by using a Cell Counting Kit‑8 assay. Cell migration and invasion were detected using a Transwell assay. Results of the present study demonstrated that the expression levels of GAS8‑AS1 in gastric cancer tissues were significantly decreased, whereas its expression did not differ among cancer tissues at different clinical stages. Low expression levels of GAS8‑AS1 predicted poor 5‑year survival rates for 70 patients with gastric adenocarcinoma from the Affiliated Hospital of Xuzhou Medical University (Xuzhou, China) during patient follow‑up. In addition, the expression levels of miR‑21‑3p were markedly increased in cancer tissues, and miR‑21‑3p expression was negatively associated with the expression of GAS8‑AS1. The direct interaction between GAS8‑AS1 and miR‑21‑3p was predicted using the starBase database and was confirmed by using an RNA pull‑down assay. In gastric cell lines, the overexpression of GAS8‑AS1 reduced the expression levels of mature miR‑21‑3p but did not affect the expression of miR‑21‑3p precursor, while the overexpression of miR‑21‑3p did not, in turn, affect the expression of GAS8‑AS1. In addition, the overexpression of GAS8‑AS1 inhibited cancer cell proliferation, while the overexpression of miR‑21‑3p promoted cancer cell proliferation and attenuated the effects of GAS8‑AS1. Overexpression of miR‑21‑3p promoted cancer cell migration and invasion, whereas overexpression of GAS8‑AS1 did not affect cell migration or invasion. In summary, results of the present study have demonstrated that GAS8‑AS1 acts as a tumor suppressor in gastric cancer, and it may inhibit cancer cell proliferation by downregulating miR‑21‑3p.

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