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WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccine

  • Authors:
    • Shouq Alzaaqi
    • Norifumi Naka
    • Kenichiro Hamada
    • Naoki Hosen
    • Mizuki Kanegae
    • Hidetatsu Outani
    • Mayuko Adachi
    • Rin Imanishi
    • Eiichi Morii
    • Miki Iwai
    • Jun Nakata
    • Fumihiro Fujiki
    • Soyoko Morimoto
    • Hiroko Nakajima
    • Sumiyuki Nishida
    • Akihiro Tsuboi
    • Yoshihiro Oka
    • Haruo Sugiyama
    • Yusuke Oji
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory and Biomedical Sciences, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan, Department of Orthopaedic Surgery, Nachikatsuura Town Onsen Hospital, Nachikatsuura, Wakayama 649‑5331, Japan, Hamada Orthopaedic Surgery, Kawanishi City, Hyogo 666‑0021, Japan, Department of Hematology and Oncology, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan, Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan, Department of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan, Department of Cancer Immunology, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan, Department of Cancer Stem Cell Biology, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan, Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan, Department of Cancer Immunotherapy, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan
    Copyright: © Alzaaqi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 65
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    Published online on: January 3, 2022
       https://doi.org/10.3892/ol.2022.13184
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Abstract

The Wilms' tumor gene WT1 is highly expressed in various malignancies and may be a common target antigen for cancer immunotherapy. In our group, peptide‑based cancer vaccines targeting WT1 CTL epitopes were developed as an immunotherapy for these malignancies. In the present study, WT1 epitope‑specific immune responses were analyzed in 31 patients with advanced sarcoma with human leukocyte antigen‑A*24:02‑ and WT1‑expressing tumors who received the WT1‑235 peptide vaccine as monotherapy. The serum levels of IgG and IgM antibodies against the target epitope WT1‑235 and the non‑target epitopes WT1‑332 and WT1‑271 were measured using ELISA. IgM antibodies against WT1‑235, WT1‑332 and WT1‑271 were detected in three (9.6%), four (12.9%) and 20 patients (64.5%), respectively, prior to vaccine administration, indicating immune recognition of the WT1 antigen prior to administering the vaccine. Of 15 patients who had completed the 3‑month treatment protocol, WT1‑235 IgG was positive in five (33.3%) patients. An enzyme‑linked immunospot assay revealed that WT1‑235 epitope‑specific IL‑10 production/secretion in peripheral blood mononuclear cells declined in the first month of vaccine administration in all three patients with positivity for WT1‑235 IgM at the start of the vaccine. Furthermore, positivity for both WT1‑235 and WT1‑271 IgM antibodies at the start of treatment was associated with unfavorable tumor control at 3 months after vaccine administration. These results suggested that WT1 epitope‑specific IgG and IgM antibodies may be utilized as immune‑monitoring markers for WT1 peptide cancer vaccine immunotherapy. The trials were entered in the University hospital Medical Information Network (UMIN) Clinical Trials Registry (https://www.umin.ac.jp/ctr; no. UMIN000002001 on May 24, 2009 and no. UMIN000015997 on December 20, 2014).
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Copy and paste a formatted citation
Spandidos Publications style
Alzaaqi S, Naka N, Hamada K, Hosen N, Kanegae M, Outani H, Adachi M, Imanishi R, Morii E, Iwai M, Iwai M, et al: WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccine. Oncol Lett 23: 65, 2022.
APA
Alzaaqi, S., Naka, N., Hamada, K., Hosen, N., Kanegae, M., Outani, H. ... Oji, Y. (2022). WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccine. Oncology Letters, 23, 65. https://doi.org/10.3892/ol.2022.13184
MLA
Alzaaqi, S., Naka, N., Hamada, K., Hosen, N., Kanegae, M., Outani, H., Adachi, M., Imanishi, R., Morii, E., Iwai, M., Nakata, J., Fujiki, F., Morimoto, S., Nakajima, H., Nishida, S., Tsuboi, A., Oka, Y., Sugiyama, H., Oji, Y."WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccine". Oncology Letters 23.2 (2022): 65.
Chicago
Alzaaqi, S., Naka, N., Hamada, K., Hosen, N., Kanegae, M., Outani, H., Adachi, M., Imanishi, R., Morii, E., Iwai, M., Nakata, J., Fujiki, F., Morimoto, S., Nakajima, H., Nishida, S., Tsuboi, A., Oka, Y., Sugiyama, H., Oji, Y."WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccine". Oncology Letters 23, no. 2 (2022): 65. https://doi.org/10.3892/ol.2022.13184
Copy and paste a formatted citation
x
Spandidos Publications style
Alzaaqi S, Naka N, Hamada K, Hosen N, Kanegae M, Outani H, Adachi M, Imanishi R, Morii E, Iwai M, Iwai M, et al: WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccine. Oncol Lett 23: 65, 2022.
APA
Alzaaqi, S., Naka, N., Hamada, K., Hosen, N., Kanegae, M., Outani, H. ... Oji, Y. (2022). WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccine. Oncology Letters, 23, 65. https://doi.org/10.3892/ol.2022.13184
MLA
Alzaaqi, S., Naka, N., Hamada, K., Hosen, N., Kanegae, M., Outani, H., Adachi, M., Imanishi, R., Morii, E., Iwai, M., Nakata, J., Fujiki, F., Morimoto, S., Nakajima, H., Nishida, S., Tsuboi, A., Oka, Y., Sugiyama, H., Oji, Y."WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccine". Oncology Letters 23.2 (2022): 65.
Chicago
Alzaaqi, S., Naka, N., Hamada, K., Hosen, N., Kanegae, M., Outani, H., Adachi, M., Imanishi, R., Morii, E., Iwai, M., Nakata, J., Fujiki, F., Morimoto, S., Nakajima, H., Nishida, S., Tsuboi, A., Oka, Y., Sugiyama, H., Oji, Y."WT1 epitope‑specific IgG and IgM antibodies for immune‑monitoring in patients with advanced sarcoma treated with a WT1 peptide cancer vaccine". Oncology Letters 23, no. 2 (2022): 65. https://doi.org/10.3892/ol.2022.13184
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