<em>CADM1</em> promotes adhesion to vascular endothelial cells and transendothelial migration in cultured GIST cells

Yuan,Jiayin; Kihara,Takako; Kimura,Neinei; Yamasaki,Takashi; Yoshida,Makoto; Isozaki,Koji; Ito,Akihiko; Hirota,Seiichi

doi:10.3892/ol.2022.13206

CADM1 promotes adhesion to vascular endothelial cells and transendothelial migration in cultured GIST cells

Authors:
- Jiayin Yuan
- Takako Kihara
- Neinei Kimura
- Takashi Yamasaki
- Makoto Yoshida
- Koji Isozaki
- Akihiko Ito
- Seiichi Hirota
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Affiliations: Department of Surgical Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo 663‑8501, Japan, Department of Pathology, Faculty of Medicine, Kindai University, Osaka‑Sayama, Osaka 589‑8511, Japan
Published online on: January 19, 2022 https://doi.org/10.3892/ol.2022.13206
Article Number: 86
Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the human gastrointestinal tract. Small intestinal GISTs appear to be associated with poorer prognosis and higher metastasis rate than gastric GISTs of the same size and mitotic index. Recently, we reported that cell adhesion molecule 1 (CADM1) is expressed specifically in most small intestinal GISTs, but not in most gastric GISTs, suggesting that this difference in CADM1 expression between gastric GISTs and small intestinal GISTs might influence the difference in clinical behavior between them. The aim of the present study was to examine whether high CADM1 expression affected proliferation, migration, invasion, adhesion to endothelial cells and transendothelial migration of cultured GIST cells by comparing original GIST‑T1 cells with very low CADM1 expression with GIST‑T1 cells with high CADM1 expression induced by CADM1 cDNA transfection (GIST‑T1‑CAD cells). GIST‑T1‑CAD cells had reduced ability to proliferate, migrate and invade compared with the original GIST‑T1 cells, but showed significantly higher ability to adhere to human umbilical vein endothelial cells and migrate through endothelial cell monolayers. Thus, CADM1 may contribute to higher metastasis rates in small intestinal GISTs facilitating tumor cell adhesion to vascular endothelial cell and transendothelial migration of tumor cells. CADM1 might serve as a potential target for inhibition of metastasis in small intestinal GISTs.

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