Open Access

BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T‑cell lymphoma

  • Authors:
    • Dongyu Bai
    • Yong Zhou
    • Fayan Shen
    • Dehong Gao
    • Wenhao Suo
    • Haiping Zhang
    • Heng Li
  • View Affiliations

  • Published online on: May 25, 2022     https://doi.org/10.3892/ol.2022.13344
  • Article Number: 223
  • Copyright: © Bai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to explore the role and biological function of bromodomain PHD finger transcription factor (BPTF) in T‑cell lymphoma. Reverse transcription‑quantitative PCR (RT‑qPCR), western blotting, immunohistochemistry and bioinformatics analysis were used to determine the expression levels of BPTF and Raf1 in T‑cell lymphoma tissues and matched adjacent normal tissues. RT‑qPCR and western blot analyses were used to examine the role of BPTF in the activation of MAPK signaling. The function of BPTF and Raf1 in T‑cell lymphoma was investigated through in vitro and in vivo assays (MTT assay, colony formation assay, flow cytometry, western blotting, tumor xenograft model and TUNEL assay) following silencing and overexpression experiments in Hut‑102 cells. The results demonstrated that BPTF and Raf1 were overexpressed in T‑cell lymphoma tissues compared with normal tissues, and high expression of BPTF or Raf1 was associated with advanced clinical stage. BPTF promoted the activation of the MAPK pathway and was coexpressed with Raf1 in T‑cell lymphoma tissues. Functional assays demonstrated that silencing of BPTF or Raf1 in Hut‑102 cells suppressed cell proliferation and induced apoptosis. Furthermore, the carcinogenic effect of BPTF was confirmed by xenograft experiments in nude mice. The present findings suggested that BPTF may function as a crucial oncogenic factor and may serve as a novel therapeutic target in T‑cell lymphoma.

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July-2022
Volume 24 Issue 1

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Spandidos Publications style
Bai D, Zhou Y, Shen F, Gao D, Suo W, Zhang H and Li H: BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T‑cell lymphoma. Oncol Lett 24: 223, 2022
APA
Bai, D., Zhou, Y., Shen, F., Gao, D., Suo, W., Zhang, H., & Li, H. (2022). BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T‑cell lymphoma. Oncology Letters, 24, 223. https://doi.org/10.3892/ol.2022.13344
MLA
Bai, D., Zhou, Y., Shen, F., Gao, D., Suo, W., Zhang, H., Li, H."BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T‑cell lymphoma". Oncology Letters 24.1 (2022): 223.
Chicago
Bai, D., Zhou, Y., Shen, F., Gao, D., Suo, W., Zhang, H., Li, H."BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T‑cell lymphoma". Oncology Letters 24, no. 1 (2022): 223. https://doi.org/10.3892/ol.2022.13344