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Association of high cellular expression and plasma concentration of angiopoietin‑like 4 with tongue cancer lung metastasis and poor prognosis

  • Authors:
    • Takuya Tanaka
    • Takahisa Imamura
    • Atsushi Irie
    • Masakazu Yoneda
    • Ryuji Imamura
    • Ken Kikuchi
    • Saki Kitagawa
    • Tatsuko Kubo
    • Hidenao Ogi
    • Hideki Nakayama
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Kumamoto 860‑8556, Japan, Department of Molecular Pathology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Kumamoto 860‑8556, Japan, Department of Immunogenetics, Faculty of Life Sciences, Kumamoto University, Kumamoto, Kumamoto 860‑8556, Japan, Department of Oral Surgery, Oral and Maxillofacial Center, Kagoshima University Hospital, Kagoshima, Kagoshima 890‑8520, Japan, Department of Urology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Kumamoto 860‑8556, Japan, Sakurajyuji Hospital, Kumamoto, Kumamoto 861‑4173, Japan, Department of Oral Surgery, Minamata Medical Center, Minamata, Kumamoto 867‑0041, Japan
    Copyright: © Tanaka et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 299
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    Published online on: July 5, 2022
       https://doi.org/10.3892/ol.2022.13419
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Abstract

Angiopoietin‑like 4 (ANGPTL4) promotes cancer cell migration through vessels and has been implicated in cancer metastasis. Our previous study identified a robust increase in ANGPTL4 mRNA expression in lung‑metastasized tongue cancer (TC) cells. Therefore, the present study investigated the association of ANGPTL4 with lung metastasis and outcomes of patient with TC. ANGPTL4 expression in TC cells was investigated by immunohistochemical staining. Patients were classified into ‘low (0‑30%)’ and ‘high (>30%)’ ANGPTL4‑expression groups based on the proportion of ANGPTL4‑positive TC cells. The high ANGPTL4‑expression group included 15 of 48 patients with TC. Notably, a significantly greater proportion of patients with lung metastasis exhibited a high rate of ANGPTL4‑expressing cancer cells compared with patients without lung metastasis (P=0.029). The overall 5‑year survival rate was lower in the high (27%) ANGPTL4‑expression group compared with the low (68%) ANGPTL4‑expression group. Univariate and multivariate analyses revealed that patients with high ANGPTL4 expression in TC cells exhibited significantly lower overall survival (OS) rates [hazard ratio (HR), 2.99; 95% confidence interval (95% CI), 1.34‑6.69; P=0.008 and HR, 2.72; 95% CI, 1.14‑6.51; P=0.024, respectively]. High plasma ANGPTL4 concentrations as measured by ELISA were associated with lung metastasis (P<0.001). The optimal cut‑point for prediction of TC lung metastasis was 9.1 ng/ml (P<0.001; 95% CI, 7.2‑10.9). The OS of patients with plasma ANPTL4 above the cut‑point was significantly lower than that of patients with plasma ANGPTL4 ≤9.1 ng/ml (P<0.001). These results suggest that a high level of ANGPTL4 in cancer cells and plasma may predict lung metastasis and/or a poor prognosis of patients with TC.
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Copy and paste a formatted citation
Spandidos Publications style
Tanaka T, Imamura T, Irie A, Yoneda M, Imamura R, Kikuchi K, Kitagawa S, Kubo T, Ogi H, Nakayama H, Nakayama H, et al: Association of high cellular expression and plasma concentration of angiopoietin‑like 4 with tongue cancer lung metastasis and poor prognosis. Oncol Lett 24: 299, 2022.
APA
Tanaka, T., Imamura, T., Irie, A., Yoneda, M., Imamura, R., Kikuchi, K. ... Nakayama, H. (2022). Association of high cellular expression and plasma concentration of angiopoietin‑like 4 with tongue cancer lung metastasis and poor prognosis. Oncology Letters, 24, 299. https://doi.org/10.3892/ol.2022.13419
MLA
Tanaka, T., Imamura, T., Irie, A., Yoneda, M., Imamura, R., Kikuchi, K., Kitagawa, S., Kubo, T., Ogi, H., Nakayama, H."Association of high cellular expression and plasma concentration of angiopoietin‑like 4 with tongue cancer lung metastasis and poor prognosis". Oncology Letters 24.3 (2022): 299.
Chicago
Tanaka, T., Imamura, T., Irie, A., Yoneda, M., Imamura, R., Kikuchi, K., Kitagawa, S., Kubo, T., Ogi, H., Nakayama, H."Association of high cellular expression and plasma concentration of angiopoietin‑like 4 with tongue cancer lung metastasis and poor prognosis". Oncology Letters 24, no. 3 (2022): 299. https://doi.org/10.3892/ol.2022.13419
Copy and paste a formatted citation
x
Spandidos Publications style
Tanaka T, Imamura T, Irie A, Yoneda M, Imamura R, Kikuchi K, Kitagawa S, Kubo T, Ogi H, Nakayama H, Nakayama H, et al: Association of high cellular expression and plasma concentration of angiopoietin‑like 4 with tongue cancer lung metastasis and poor prognosis. Oncol Lett 24: 299, 2022.
APA
Tanaka, T., Imamura, T., Irie, A., Yoneda, M., Imamura, R., Kikuchi, K. ... Nakayama, H. (2022). Association of high cellular expression and plasma concentration of angiopoietin‑like 4 with tongue cancer lung metastasis and poor prognosis. Oncology Letters, 24, 299. https://doi.org/10.3892/ol.2022.13419
MLA
Tanaka, T., Imamura, T., Irie, A., Yoneda, M., Imamura, R., Kikuchi, K., Kitagawa, S., Kubo, T., Ogi, H., Nakayama, H."Association of high cellular expression and plasma concentration of angiopoietin‑like 4 with tongue cancer lung metastasis and poor prognosis". Oncology Letters 24.3 (2022): 299.
Chicago
Tanaka, T., Imamura, T., Irie, A., Yoneda, M., Imamura, R., Kikuchi, K., Kitagawa, S., Kubo, T., Ogi, H., Nakayama, H."Association of high cellular expression and plasma concentration of angiopoietin‑like 4 with tongue cancer lung metastasis and poor prognosis". Oncology Letters 24, no. 3 (2022): 299. https://doi.org/10.3892/ol.2022.13419
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