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SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A

  • Authors:
    • Boya Deng
    • Ailin Li
    • Ying Zhu
    • Yingying Zhou
    • Jing Fei
    • Yuan Miao
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The Second Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Pathology, The College of Basic Medicine Science and The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 246
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    Published online on: April 21, 2023
       https://doi.org/10.3892/ol.2023.13832
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Abstract

Cervical cancer (CC) is the most common human papillomavirus‑related disease. Continuous activation of the NF‑κB signaling pathway has been observed in CC. SHC binding and spindle associated 1 (SHCBP1) contributes to tumorigenesis and activation of the NF‑κB pathway in multiple cancer types, while its function in CC remains unclear. In the present study, three Gene Expression Omnibus datasets were used to identify differentially expressed genes (DEGs) in CC. Loss‑ and gain‑of‑function experiments were performed using stable SHCBP1‑silenced and SHCBP1‑overexpressing CC cells. To further explore the molecular mechanism of SHCBP1 in CC, small interfering RNA targeting eukaryotic translation initiation factor 5A (EIF5A) was transfected into stable SHCBP1‑overexpressing CC cells. The results demonstrated that SHCBP1 was an upregulated DEG in CC tissues compared with healthy control cervical tissues. Functional experiments revealed the pro‑proliferative and pro‑stemness role of SHCBP1 in CC cells (CaSki and SiHa cells), in vitro. Furthermore, the NF‑κB signaling pathway in CC cells was activated by SHCBP1. Increases in cell proliferation, stemness and activation of NF‑κB, induced by SHCBP1 overexpression in CC cells, were reversed by EIF5A knockdown. Taken together, the results indicated that SHCBP1 serves an important role in regulation of CC cell proliferation, self‑renewal and activation of NF‑κB via EIF5A. The present study demonstrated a potential molecular mechanism underlying the progression of CC.
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Copy and paste a formatted citation
Spandidos Publications style
Deng B, Li A, Zhu Y, Zhou Y, Fei J and Miao Y: SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A. Oncol Lett 25: 246, 2023.
APA
Deng, B., Li, A., Zhu, Y., Zhou, Y., Fei, J., & Miao, Y. (2023). SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A. Oncology Letters, 25, 246. https://doi.org/10.3892/ol.2023.13832
MLA
Deng, B., Li, A., Zhu, Y., Zhou, Y., Fei, J., Miao, Y."SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A". Oncology Letters 25.6 (2023): 246.
Chicago
Deng, B., Li, A., Zhu, Y., Zhou, Y., Fei, J., Miao, Y."SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A". Oncology Letters 25, no. 6 (2023): 246. https://doi.org/10.3892/ol.2023.13832
Copy and paste a formatted citation
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Spandidos Publications style
Deng B, Li A, Zhu Y, Zhou Y, Fei J and Miao Y: SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A. Oncol Lett 25: 246, 2023.
APA
Deng, B., Li, A., Zhu, Y., Zhou, Y., Fei, J., & Miao, Y. (2023). SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A. Oncology Letters, 25, 246. https://doi.org/10.3892/ol.2023.13832
MLA
Deng, B., Li, A., Zhu, Y., Zhou, Y., Fei, J., Miao, Y."SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A". Oncology Letters 25.6 (2023): 246.
Chicago
Deng, B., Li, A., Zhu, Y., Zhou, Y., Fei, J., Miao, Y."SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A". Oncology Letters 25, no. 6 (2023): 246. https://doi.org/10.3892/ol.2023.13832
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