Open Access

Bone mesenchymal stem cell‑derived exosome‑encapsulated microRNA‑125b‑5p inhibits ovarian cancer progression via DDX5 downregulation

  • Authors:
    • Yuxia Wang
    • Wei Wang
    • Dan Zheng
    • Ying Gao
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  • Published online on: April 1, 2025     https://doi.org/10.3892/ol.2025.15001
  • Article Number: 255
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Exosomes can be used to mediate the delivery of nucleic acids such as microRNA‑125b‑5p (miR‑125b‑5p), a tumor‑suppressor in certain types of cancer, into tumor cells. The present study investigated the use of bone mesenchymal stem cells‑derived exosome (BMSCs‑Exo) delivery of miR‑125b‑5p in ovarian cancer (OC). BMSCs were transfected with miR‑125b‑5p mimic, from which exosomes termed Exo‑miR‑125b‑5p mimic were extracted. The expression levels of miR‑125b‑5p in OC tissue samples, BMSCs, exosomes and SKOV3 cells were quantified using reverse transcription‑quantitative PCR. The influence of Exo‑miR‑125b‑5p mimic on the biological functions of OC was evaluated through cell proliferation, invasion, migration and apoptosis assays. The targeting relationship between miR‑125b‑5p and DEAD‑box helicase 5 (DDX5) was verified, and the expression levels of DDX5 in OC samples and SKOV3 cells were quantified using western blotting. miR‑125b‑5p was downregulated in tumor tissue samples from patients with OC. BMSCs‑Exo reduced the malignant properties of SKOV3 cells in vitro, and these effects were be advanced by miR‑125b‑5p upregulation. miR‑125b‑5p targeted and inhibited DDX5 expression. DDX5 overexpression inhibited Exo‑miR‑125b‑5p‑induced suppression of OC development. Overall, this study highlights that BMSCs‑Exo‑encapsulated miR‑125b‑5p inhibited OC progression via DDX5 downregulation, providing insight into the molecular mechanisms underlying OC.
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May-2025
Volume 29 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Wang Y, Wang W, Zheng D and Gao Y: Bone mesenchymal stem cell‑derived exosome‑encapsulated microRNA‑125b‑5p inhibits ovarian cancer progression via DDX5 downregulation. Oncol Lett 29: 255, 2025.
APA
Wang, Y., Wang, W., Zheng, D., & Gao, Y. (2025). Bone mesenchymal stem cell‑derived exosome‑encapsulated microRNA‑125b‑5p inhibits ovarian cancer progression via DDX5 downregulation. Oncology Letters, 29, 255. https://doi.org/10.3892/ol.2025.15001
MLA
Wang, Y., Wang, W., Zheng, D., Gao, Y."Bone mesenchymal stem cell‑derived exosome‑encapsulated microRNA‑125b‑5p inhibits ovarian cancer progression via DDX5 downregulation". Oncology Letters 29.5 (2025): 255.
Chicago
Wang, Y., Wang, W., Zheng, D., Gao, Y."Bone mesenchymal stem cell‑derived exosome‑encapsulated microRNA‑125b‑5p inhibits ovarian cancer progression via DDX5 downregulation". Oncology Letters 29, no. 5 (2025): 255. https://doi.org/10.3892/ol.2025.15001