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Article Open Access

Protein arginine methyltransferase 7 regulates the cell cycle and promotes the progression of prostate cancer

  • Authors:
    • Hongyuan Wan
    • Hangshen Zhou
    • Jiandong Gui
    • Dongjie Yang
    • Rong Wang
    • Jiang Ni
    • Sheng Wu
    • Yan Qin
    • Qiaowei Qi
    • Lijie Zhu
    • Ninghan Feng
    • Yuanyuan Mi
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214122, P.R. China, Department of Pathology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214122, P.R. China, Institute of Integrated Traditional Chinese and Western Medicine, Wuxi Medical College, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China, Department of Pharmacy, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214122, P.R. China, Department of Urology, Jiangnan University Medical Center, Wuxi, Jiangsu 214043, P.R. China
    Copyright: © Wan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 549
    |
    Published online on: September 23, 2025
       https://doi.org/10.3892/ol.2025.15295
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Abstract

Prostate cancer (PCa) is the second most common cancer in men worldwide. Protein arginine methyltransferase 7 (PRMT7) expression is associated with tumor growth, as it can drive tumor cell proliferation and promote its invasiveness in several types of cancer. However, its mechanism in PCa remains to be elucidated. In the present study, the function and associated mechanism of PRMT7 in PCa cells were investigated. The relationship between PRMT7 and PCa was analyzed using The Cancer Genome Atlas online database. Tissue chip techniques were used to identify the clinical relevance of PRMT7 expression. PRMT7 expression levels in PCa tissues and cells were verified using reverse transcription‑quantitative PCR (RT‑qPCR). Cell cycle, migration, proliferation and apoptosis of PC3 and DU145 cells were observed using flow cytometry, Cell Counting Kit‑8, wound healing, plate cloning and cell invasion assays. Gene set enrichment analysis and chip expression profiles were used to predict the potential signaling pathway involved in the action of PRMT7 in PCa. First, The Cancer Genome Atlas database, tissue microarray analysis and RT‑qPCR revealed that PRMT7 expression was increased in PCa tissues and cells. Furthermore, small interfering RNA‑mediated PRMT7 knockdown led to a notable reduction in the proliferation of cells, increased apoptosis, affected the cell cycle and decreased cell migration and invasion. Furthermore, PRMT7 regulated the functions of Yin Yang 1 (YY1), tumor protein p53 (TP53), cyclin D2 (CCND2), CDK6 and retinoblastoma 1 (RB1) in PCa. PRMT7 may promote proliferation, migration and metastasis in PCa cells by regulating the activity of YY1, TP53, CCND2, CDK6 and RB1 in the cell cycle signaling pathway.
View Figures

Figure 1

Differential gene expression patterns
of PRMT7 in PCa. (A) The correlation between PRMT7 expression in
cancerous and adjacent tissues of patients with PCa in TCGA
database. (B) The correlation between PRMT7 expression and age of
patients with PCa in TCGA database. (C) The correlation between
PRMT7 expression and Gleason score of patients with PCa in TCGA
database. (D) The correlation between PRMT7 expression and PSA
levels of patients with PCa in TCGA database. The correlation
between PRMT7 expression and (E) T, (F) N and (G) M stage of
patients with PCa in TCGA database. (H) The correlation between
PRMT7 expression and OS event of patients with PCa in TCGA
database. (I) Representative image of PRMT7 immunohistochemical
staining of PCa tissues and adjacent normal tissues (magnification,
50 and 100×; scale bar, 40 and 10 µm). The red arrows indicate the
PRMT7 positive areas. (J) The expression levels of PRMT7 in PCa
tissues was detected using RT-qPCR. The blue circle represents the
normal tissue around the cancer, while the red arrow represents the
tumor tissue. (K) The expression level of PRMT7 in PCa cell lines
and normal prostate cells was detected using RT-qPCR. *P<0.05,
**P<0.01 and ***P<0.001. PRMT7, protein arginine
methyltransferase 7; PCa, prostate cancer; PSA, protein specific
antigen; TPM, transcript per million; TCGA, The Cancer Genome
Atlas; RT-qPCR, reverse transcription-quantitative PCR; ns, not
significant.

Figure 2

Function of PRMT7 in the PC3 and
DU145 cell lines. (A) The inhibition efficiency of siPRMT7 in cell
lines was determined using RT-qPCR. (B) Western blotting was used
to detect the protein expression levels of PRMT7 in the siPRMT7 and
siCtrl groups. (C) Cell proliferation in the siCtrl and siPRMT7
groups was assessed using a Cell Counting Kit-8 assay. (D) A wound
healing assay was used to investigate the migratory ability of PCa
cells after PRMT7 knockdown (scale bar, 500 µm). (E) Plate colony
formation assays were performed to determine the proliferation
abilities of PC3 and DU145 cells treated with siCtrl and siPRMT7.
The (F) migration and (G) invasion of PC3 and DU145 cells were
investigated using a Transwell assay after PRMT7 knockdown (scale
bar, 100 µm). (H) PI-FACS detection of the effect of PRMT7
knockdown on the PCa cell cycle. (I) The effect of PRMT7 knockdown
on the apoptosis of PCa cells was detected using Annexin V-APC
single staining. *P<0.05, **P<0.01 and ***P<0.001. PRMT7,
protein arginine methyltransferase 7; PCa, prostate cancer;
RT-qPCR, reverse transcription-quantitative PCR; siRNA, small
interfering RNA; si, siRNA; Ctrl; control; NC, negative control;
CCK-8, Cell Counting Kit-8.

Figure 3

Transcriptome sequencing reveals
differential gene expression after PRMT7 knockdown. (A) PRMT7
expression differences among transcriptome sequencing samples were
detected using RT-qPCR. (B) Sample gene expression distribution box
diagram. After calculating the FPKM values for all genes in each
sample, the distribution of gene expression levels across different
samples were visualized using boxplots. (C) Co-expression Venn
diagram demonstrates the number of genes uniquely expressed in each
group/sample. The overlapping regions indicate the number of
co-expressed genes in two or more groups/samples. The comparison of
the number of differentially expressed genes is displayed in the
form of (D) volcano plot and (E) statistical histogram. Volcano
plot displaying DEGs. The x-axis demonstrates log2FC
between the treatment and control groups and the y-axis represents
the adjusted P-value (−log10Adjusted P). Red dots denote
significantly upregulated genes (log2FC>1; Adjusted
P<0.05), while green dots indicate downregulated genes
(log2FC<-1; Adjusted P<0.05). PRMT7, protein
arginine methyltransferase 7; DEGs; differentially expressed genes;
FPKM, fragments per KB of transcript per million mapped reads;
log2FC, log2 fold change; RT-qPCR, reverse
transcription-quantitative PCR; si, small interfering.

Figure 4

PRMT7 is involved in the regulation
of cell cycle-related pathways and affects the cell cycle in PCa.
(A) The DO enrichment distribution map of the DEGs associated with
PRMT7. DO is a biomedical database that systematically annotates
associations between human gene functions and diseases, providing
standardized disease descriptors for functional genomics research.
(B) Map of the GO enrichment sites of the DEGs associated with
PRMT7. GO is a comprehensive bioinformatics resource that
systematically characterizes gene functions through three
orthogonal categories: Biological processes, cellular components
and molecular functions. (C) KEGG enrichment analysis of the DEGs
associated with PRMT7. KEGG is an integrated database that
systematically consolidates genomic, chemical and systems
functional information to facilitate biological pathway analysis
and network modeling. (D) KEGG enrichment and distribution map of
the PRMT7 differential genes. PRMT7, protein arginine
methyltransferase 7; DEGs, Differentially expressed genes;
log2FC, log2 fold change; DO, Disease
Ontology; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and
Genomes; NAFLD, Non-alcoholic fatty liver disease; NOD,
Nucleotide-binding Oligomerization Domain.

Figure 5

PRMT7 is involved in the
YY1/TP53/CCND2/CDK6/RB1 cell cycle signaling axis. (A) Heatmaps of
cell cycle-related differential genes identified using
transcriptome sequencing. (B) Cell cycle-related regulatory
proteins predicted in the STRING database and their component
signaling axes. PRMT7, protein arginine methyltransferase 7; YY1,
Yin Yang 1; TP53, tumor protein p53; CCND2, cyclin D2; RB1,
retinoblastoma 1; STRING, Search Tool for the Retrieval of
Interacting Genes/Proteins.
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Copy and paste a formatted citation
Spandidos Publications style
Wan H, Zhou H, Gui J, Yang D, Wang R, Ni J, Wu S, Qin Y, Qi Q, Zhu L, Zhu L, et al: Protein arginine methyltransferase 7 regulates the cell cycle and promotes the progression of prostate cancer. Oncol Lett 30: 549, 2025.
APA
Wan, H., Zhou, H., Gui, J., Yang, D., Wang, R., Ni, J. ... Mi, Y. (2025). Protein arginine methyltransferase 7 regulates the cell cycle and promotes the progression of prostate cancer. Oncology Letters, 30, 549. https://doi.org/10.3892/ol.2025.15295
MLA
Wan, H., Zhou, H., Gui, J., Yang, D., Wang, R., Ni, J., Wu, S., Qin, Y., Qi, Q., Zhu, L., Feng, N., Mi, Y."Protein arginine methyltransferase 7 regulates the cell cycle and promotes the progression of prostate cancer". Oncology Letters 30.6 (2025): 549.
Chicago
Wan, H., Zhou, H., Gui, J., Yang, D., Wang, R., Ni, J., Wu, S., Qin, Y., Qi, Q., Zhu, L., Feng, N., Mi, Y."Protein arginine methyltransferase 7 regulates the cell cycle and promotes the progression of prostate cancer". Oncology Letters 30, no. 6 (2025): 549. https://doi.org/10.3892/ol.2025.15295
Copy and paste a formatted citation
x
Spandidos Publications style
Wan H, Zhou H, Gui J, Yang D, Wang R, Ni J, Wu S, Qin Y, Qi Q, Zhu L, Zhu L, et al: Protein arginine methyltransferase 7 regulates the cell cycle and promotes the progression of prostate cancer. Oncol Lett 30: 549, 2025.
APA
Wan, H., Zhou, H., Gui, J., Yang, D., Wang, R., Ni, J. ... Mi, Y. (2025). Protein arginine methyltransferase 7 regulates the cell cycle and promotes the progression of prostate cancer. Oncology Letters, 30, 549. https://doi.org/10.3892/ol.2025.15295
MLA
Wan, H., Zhou, H., Gui, J., Yang, D., Wang, R., Ni, J., Wu, S., Qin, Y., Qi, Q., Zhu, L., Feng, N., Mi, Y."Protein arginine methyltransferase 7 regulates the cell cycle and promotes the progression of prostate cancer". Oncology Letters 30.6 (2025): 549.
Chicago
Wan, H., Zhou, H., Gui, J., Yang, D., Wang, R., Ni, J., Wu, S., Qin, Y., Qi, Q., Zhu, L., Feng, N., Mi, Y."Protein arginine methyltransferase 7 regulates the cell cycle and promotes the progression of prostate cancer". Oncology Letters 30, no. 6 (2025): 549. https://doi.org/10.3892/ol.2025.15295
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