Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
December-2025 Volume 30 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2025 Volume 30 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data1.pdf
    • Supplementary_Data2.pdf
Article Open Access

SBEM confers paclitaxel resistance in breast cancer via DUSP16‑mediated MAPK/AMPK pathway activation

  • Authors:
    • Liang Liu
    • Ning Lu
    • Xiaomei Liu
    • Qingting Yi
    • Shengnan Li
    • Jie Wen
    • Huijuan Sun
    • Yuanming Du
  • View Affiliations / Copyright

    Affiliations: Graduate School, Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830000, P.R. China, Department of Oncology, Xinjiang Military Command General Hospital of PLA, Urumqi, Xinjiang Uygur Autonomous Region 830000, P.R. China, Department of Oncology, Qingdao West Coast New Area People's Hospital, Qingdao, Shandong 266000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 607
    |
    Published online on: October 21, 2025
       https://doi.org/10.3892/ol.2025.15353
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Small breast epithelial mucin (SBEM) is upregulated in primary breast tumors and metastatic lymph nodes and has been implicated in chemoresistance. While it has been identified as a potential biomarker for monitoring hematogenous micrometastasis in patients with breast cancer undergoing adjuvant chemotherapy, the molecular mechanisms by which SBEM confers this resistance remain unclear. Therefore, the present study aimed to elucidate the mechanisms by which SBEM regulates paclitaxel (PTX) resistance in breast cancer. To this aim, breast cancer cell lines with SBEM overexpression and knockdown were developed from parental drug‑resistant strains. Additionally, AMPK activator 13 was used to investigate the involvement of the AMPK pathway in the SBEM‑mediated effects. The results indicated that SBEM overexpression promoted cell viability and enhanced resistance to PTX in breast cancer cells. Conversely, SBEM knockdown significantly increased apoptosis, with a three‑fold increase compared with the controls, and restored PTX sensitivity in drug‑resistant cells. Mechanistically, SBEM was found to interact with dual‑specificity phosphatase 16 (DUSP16) and upregulate its expression. Additionally, SBEM downregulation inhibited AMPK signaling activity, thereby suppressing cancer cell viability. In conclusion, abnormal activation of the AMPK signaling pathway was shown to contribute to PTX resistance in breast cancer. SBEM enhanced DUSP16 expression and activated the AMPK signaling pathway, thereby conferring resistance to PTX.
View Figures

Figure 1

Inhibition of breast cancer cell
viability by PTX and the construction of PTX-resistant strains. (A)
2D molecular structure of PTX. (B) Relative expression levels of
SBEM mRNA in MCF10A, SUM190PT, BT474, AU565, SKBR3 and MCF7 cells
were measured using reverse transcription-quantitative polymerase
chain reaction. (C) CCK-8 assay showing changes in the viability of
SUM190PT and SKBR3 cells treated with varying PTX concentrations.
(D) CCK-8 assay showing changes in the viability of SUM190PT/PTX
and SKBR3/PTX cells treated with varying PTX concentrations.
*P<0.05, **P<0.01, ****P<0.0001. ns, not significant; PTX,
paclitaxel; SBEM, small breast epithelial mucin; CCK-8, Cell
Counting Kit-8.

Figure 2

Changes in SBEM expression and the
effect on PTX resistance. (A) Western blotting and RT-qPCR analyses
of SBEM protein and mRNA levels in SUM190PT/PTX cells 48 h
post-transfection with siSBEM. (B) Western blotting and RT-qPCR
analyses of SBEM protein and mRNA levels in SKBR3 cells 48 h
post-transfection with the SBEM overexpression plasmid. (C)
SUM190PT cells were treated with PTX (500 nmol/l) for 48 h. In the
case of SUM190PT/PTX cells, the PTX concentration in the
drug-resistant medium was maintained at 500 nmol/l. Subsequently,
siRNA (SBEM) was transfected for 48 h. The CCK-8 assay was used to
detect the changes in cell viability of SUM190PT and SUM190PT/PTX
cells after treatment. (D) SKBR3 cells were treated with PTX (500
nmol/l) for 48 h. Subsequently, the cells were transfected with the
SBEM-overexpressing plasmid and incubated for another 48 h. For
SKBR3/PTX cells, the PTX concentration in the drug-resistant medium
was maintained at 500 nmol/l. The CCK-8 assay was used to detect
the changes in cell viability of SKBR3 and SKBR3/PTX cells after
treatment. ***P<0.001, ****P<0.0001. ns, not significant;
PTX, paclitaxel; SBEM, small breast epithelial mucin; RT-qPCR,
reverse transcription-quantitative polymerase chain reaction;
CCK-8, Cell Counting Kit-8; si, small interfering (RNA); NC,
negative control; oe, overexpression.

Figure 3

SBEM interacts with DUSP16. (A)
Molecular docking analysis showing the interaction between PTX and
SBEM. (B) Western blot analysis of the SBEM and p-DUSP16 protein
levels in the SBEM protein complex, extracted by
immunoprecipitation following siSBEM transfection for 48 h of
SUM190PT/PTX cells. PTX, paclitaxel; SBEM, small breast epithelial
mucin; DUSP16, dual specificity phosphatase 16; p-, phosphorylated;
si, small interfering (RNA); NC, negative control.

Figure 4

SBEM modulates PTX-induced apoptosis
in breast cancer cells. (A) Flow cytometry analysis of apoptosis in
SUM190PT/PTX and SUM190PT cells following various treatments. (B)
Flow cytometry analysis of apoptosis in SKBR3/PTX and SKBR3 cells
following various treatments. (C) Western blot analysis of Bax,
Bcl2, Caspase-3 and cleaved-Caspase-3 in SUM190PT/PTX and SUM190PT
cells. (D) Western blot analysis of Bax, Bcl2, Caspase-3 and
cleaved-Caspase-3 in SKBR3/PTX and SKBR3 cells. *P<0.05,
**P<0.01, ***P<0.001, ****P<0.0001. ns, not significant;
PTX, paclitaxel; SBEM, small breast epithelial mucin; si, small
interfering (RNA); NC, negative control; oe, overexpression.

Figure 5

SBEM regulates p-DUSP16 and the MAPK
signaling pathway. (A) Western blotting of DUSP16, p-DUSP16, MAPK,
p-MAPK and SBEM in SUM190PT and SUM190PT/PTX cells. (B) Western
blotting of DUSP16, p-DUSP16, MAPK, p-MAPK and SBEM in SKBR3 and
SKBR3/PTX cells. *P<0.05, **P<0.01, ***P<0.001,
****P<0.0001. ns, not significant. PTX, paclitaxel; SBEM, small
breast epithelial mucin; DUSP16, dual specificity phosphatase 16;
p-, phosphorylated; si, small interfering (RNA); NC, negative
control; oe, overexpression,

Figure 6

SBEM induces PTX resistance in breast
cancer cells by activating the AMPK pathway. (A) Flow cytometry
analysis of apoptosis in SUM190PT/PTX cells transfected with siRNA
for 48 h and treated with AMPK activator 13 (5 µmol/l) for 24 h.
(B) The treatment of SUM190PT/PTX cells was the same as
aforementioned. After the treatment, the change in cell viability
was determined by the CCK-8 assay. (C) The treatment of
SUM190PT/PTX cells was the same as aforementioned. After the
treatment, the relative expression levels of Bax, Bcl2, Caspase-3
and cleaved-Caspase-3 proteins were detected by western blotting.
**P<0.01, ***P<0.001, ****P<0.0001. ns, not significant;
PTX, paclitaxel; SBEM, small breast epithelial mucin; CCK-8, Cell
Counting Kit-8; si, small interfering (RNA).

Figure 7

AMPK activator 13 restores drug
sensitivity to PTX. Western blotting of DUSP16, p-DUSP16, MAPK,
p-MAPK and SBEM protein levels in SUM190PT/PTX cells transfected
with siRNA for 48 h and treated with AMPK activator 13 for 24 h.
**P<0.01, ***P<0.001, ****P<0.0001. ns, not significant;
PTX, paclitaxel; SBEM, small breast epithelial mucin; DUSP16, dual
specificity phosphatase 16; p-, phosphorylated; si, small
interfering (RNA).
View References

1 

Wilkinson L and Gathani T: Understanding breast cancer as a global health concern. Br J Radiol. 95:202110332022. View Article : Google Scholar

2 

DeSantis CE, Ma J, Gaudet MM, Newman LA, Miller KD, Goding Sauer A, Jemal A and Siegel RL: Breast cancer statistics, 2019. CA Cancer J Clin. 69:438–451. 2019. View Article : Google Scholar : PubMed/NCBI

3 

Arnold M, Morgan E, Rumgay H, Mafra A, Singh D, Laversanne M, Vignat J, Gralow JR, Cardoso F, Siesling S and Soerjomataram I: Current and future burden of breast cancer: Global statistics for 2020 and 2040. Breast. 66:15–23. 2022. View Article : Google Scholar

4 

Burstein HJ, Curigliano G, Thürlimann B, Weber WP, Poortmans P, Regan MM, Senn HJ, Winer EP and Gnant M; Panelists of the St Gallen Consensus Conference, : Customizing local and systemic therapies for women with early breast cancer: The St. Gallen International Consensus Guidelines for treatment of early breast cancer 2021. Ann Oncol. 32:1216–1235. 2021. View Article : Google Scholar

5 

Abu Samaan TM, Samec M, Liskova A, Kubatka P and Büsselberg D: Paclitaxel's mechanistic and clinical effects on breast cancer. Biomolecules. 9:7892019. View Article : Google Scholar : PubMed/NCBI

6 

Dan VM, Raveendran RS and Baby S: Resistance to intervention: Paclitaxel in breast cancer. Mini Rev Med Chem. 21:1237–1268. 2021. View Article : Google Scholar : PubMed/NCBI

7 

Zhang Y, Lun X and Guo W: Expression of TRPC1 and SBEM protein in breast cancer tissue and its relationship with clinicopathological features and prognosis of patients. Oncol Lett. 20:3922020.

8 

Hao H, Yang L, Wang B, Sang Y and Liu X: Small breast epithelial mucin as a useful prognostic marker for breast cancer patients. Open Life Sci. 18:202207842023. View Article : Google Scholar

9 

Hao H, Wang B, Yang L, Sang Y, Xu W, Liu W, Zhang L and Jiang D: miRNA-186-5p inhibits migration, invasion and proliferation of breast cancer cells by targeting SBEM. Aging (Albany NY). 15:6993–7007. 2023. View Article : Google Scholar : PubMed/NCBI

10 

Li QH, Liu ZZ, Ge Y, Liu X, Xie XD, Zheng ZD, Ma YH and Liu B: Small breast epithelial mucin promotes the invasion and metastasis of breast cancer cells via promoting epithelial-to-mesenchymal transition. Oncol Rep. 44:509–518. 2020. View Article : Google Scholar : PubMed/NCBI

11 

Liu ZZ, Xie XD, Qu SX, Zheng ZD and Wang YK: Small breast epithelial mucin (SBEM) has the potential to be a marker for predicting hematogenous micrometastasis and response to neoadjuvant chemotherapy in breast cancer. Clin Exp Metastasis. 27:251–259. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Wen S, Hou Y, Fu L, Xi L, Yang D, Zhao M, Qin Y, Sun K, Teng Y and Liu M: Cancer-associated fibroblast (CAF)-derived IL32 promotes breast cancer cell invasion and metastasis via integrin β3-p38 MAPK signalling. Cancer Lett. 442:320–332. 2019. View Article : Google Scholar

13 

Butti R, Das S, Gunasekaran VP, Yadav AS, Kumar D and Kundu GC: Receptor tyrosine kinases (RTKs) in breast cancer: Signaling, therapeutic implications and challenges. Mol Cancer. 17:342018. View Article : Google Scholar : PubMed/NCBI

14 

Ren C, Han X, Lu C, Yang T, Qiao P, Sun Y and Yu Z: Ubiquitination of NF-κB p65 by FBXW2 suppresses breast cancer stemness, tumorigenesis, and paclitaxel resistance. Cell Death Differ. 29:381–392. 2022. View Article : Google Scholar : PubMed/NCBI

15 

Zhou Y, Pang J, Liu H, Cui W, Cao J and Shi G: Fibronectin type III domain-containing protein 5 promotes autophagy via the AMPK/mTOR signaling pathway in hepatocellular carcinoma cells, contributing to nab-paclitaxel chemoresistance. Med Oncol. 40:532022. View Article : Google Scholar

16 

Zhao PW, Cui JX and Wang XM: Upregulation of p300 in paclitaxel-resistant TNBC: Implications for cell proliferation via the PCK1/AMPK axis. Pharmacogenomics J. 24:52024. View Article : Google Scholar : PubMed/NCBI

17 

Abedini MR, Muller EJ, Bergeron R, Gray DA and Tsang BK: Akt promotes chemoresistance in human ovarian cancer cells by modulating cisplatin-induced, p53-dependent ubiquitination of FLICE-like inhibitory protein. Oncogene. 29:11–25. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Caunt CJ and Keyse SM: Dual-specificity MAP kinase phosphatases (MKPs): Shaping the outcome of MAP kinase signalling. FEBS J. 280:489–504. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Cargnello M and Roux PP: Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases. Microbiol Mol Biol Rev. 75:50–83. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Roux PP and Blenis J: ERK and p38 MAPK-activated protein kinases: A family of protein kinases with diverse biological functions. Microbiol Mol Biol Rev. 68:320–344. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Hoornaert I, Marynen P, Goris J, Sciot R and Baens M: MAPK phosphatase DUSP16/MKP-7, a candidate tumor suppressor for chromosome region 12p12-13, reduces BCR-ABL-induced transformation. Oncogene. 22:7728–7736. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Low HB and Zhang Y: Regulatory roles of MAPK phosphatases in cancer. Immune Netw. 16:85–98. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Lu H, Tran L, Park Y, Chen I, Lan J, Xie Y and Semenza GL: Reciprocal regulation of DUSP9 and DUSP16 expression by HIF1 controls ERK and p38 MAP kinase activity and mediates chemotherapy-induced breast cancer stem cell enrichment. Cancer Res. 78:4191–4202. 2018. View Article : Google Scholar : PubMed/NCBI

24 

Keyse SM: Dual-specificity MAP kinase phosphatases (MKPs) and cancer. Cancer Metastasis Rev. 27:253–261. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Polák L, Škoda P, Riedlová K, Krivák R, Novotný M and Hoksza D: PrankWeb 4: A modular web server for Protein-ligand binding site prediction and downstream analysis. Nucleic Acids Res. 53:W466–W471. 2025. View Article : Google Scholar

26 

Shi Y, Wang J, Tao S, Zhang S, Mao L, Shi X, Wang W, Cheng C, Shi Y and Yang Q: miR-142-3p improves paclitaxel sensitivity in resistant breast cancer by inhibiting autophagy through the GNB2-AKT-mTOR pathway. Cell Signal. 103:1105662023. View Article : Google Scholar

27 

Mishra T, Gupta S, Rai P, Khandelwal N, Chourasiya M, Kushwaha V, Singh A, Varshney S, Gaikwad AN and Narender T: Anti-adipogenic action of a novel oxazole derivative through activation of AMPK pathway. Eur J Med Chem. 262:1158952023. View Article : Google Scholar : PubMed/NCBI

28 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Zhu Y, Wang A, Zhang S, Kim J, Xia J, Zhang F, Wang D, Wang Q and Wang J: Paclitaxel-loaded ginsenoside Rg3 liposomes for drug-resistant cancer therapy by dual targeting of the tumor microenvironment and cancer cells. J Adv Res. 49:159–173. 2023. View Article : Google Scholar : PubMed/NCBI

30 

Fleisher TA: Apoptosis. Ann Allergy Asthma Immunol. 78:245–950. 1997. View Article : Google Scholar

31 

Wu M, Xue L, Chen Y, Tang W, Guo Y, Xiong J, Chen D, Zhu Q, Fu F and Wang S: Inhibition of checkpoint kinase prevents human oocyte apoptosis induced by chemotherapy and allows enhanced tumour chemotherapeutic efficacy. Hum Reprod. 38:1769–1783. 2023. View Article : Google Scholar : PubMed/NCBI

32 

Wang BR, Han JB, Jiang Y, Xu S, Yang R, Kong YG, Tao ZZ, Hua QQ, Zou Y and Chen SM: CENPN suppresses autophagy and increases paclitaxel resistance in nasopharyngeal carcinoma cells by inhibiting the CREB-VAMP8 signaling axis. Autophagy. 20:329–348. 2024. View Article : Google Scholar : PubMed/NCBI

33 

Habib TN, Altonsy MO, Ghanem SA, Salama MS and Hosny MA: Optimizing combination therapy in prostate cancer: Mechanistic insights into the synergistic effects of Paclitaxel and Sulforaphane-induced apoptosis. BMC Mol Cell Biol. 25:52024. View Article : Google Scholar

34 

Lin YW, Lin TT, Chen CH, Wang RH, Lin YH, Tseng TY, Zhuang YJ, Tang SY, Lin YC, Pang JY, et al: Enhancing efficacy of Albumin-bound paclitaxel for human lung and colorectal cancers through autophagy receptor sequestosome 1 (SQSTM1)/p62-mediated nanodrug delivery and cancer therapy. ACS Nano. 17:19033–19051. 2023. View Article : Google Scholar : PubMed/NCBI

35 

Albuquerque T, Neves AR, Paul M, Biswas S, Vuelta E, García-Tuñón I, Sánchez-Martin M, Quintela T and Costa D: A Potential effect of circadian rhythm in the Delivery/therapeutic performance of Paclitaxel-dendrimer nanosystems. J Funct Biomater. 14:3622023. View Article : Google Scholar : PubMed/NCBI

36 

Kim JH, Lee JO, Kim N, Lee HJ, Lee YW, Kim HI, Kim SJ, Park SH and Kim HS: Paclitaxel suppresses the viability of breast tumor MCF7 cells through the regulation of EF1α and FOXO3a by AMPK signaling. Int J Oncol. 47:1874–1880. 2015. View Article : Google Scholar

37 

Tang Z, Zhang Y, Yu Z and Luo Z: Metformin suppresses stemness of Non-small-cell lung cancer induced by paclitaxel through FOXO3a. Int J Mol Sci. 24:166112023. View Article : Google Scholar

38 

Yuan J, Dong X, Yap J and Hu J: The MAPK and AMPK signalings: Interplay and implication in targeted cancer therapy. J Hematol Oncol. 13:1132020. View Article : Google Scholar

39 

Chu J, Panfen E, Wang L, Marino A, Chen XQ, Fancher RM, Landage R, Patil O, Desai SD, Shah D, et al: Evaluation of encequidar as an intestinal P-gp and BCRP specific inhibitor to assess the role of intestinal P-gp and BCRP in Drug-drug interactions. Pharm Res. 40:2567–2584. 2023. View Article : Google Scholar : PubMed/NCBI

40 

Rieske P, Krynska B and Azizi SA: Human Fibroblast-derived cell lines have characteristics of embryonic stem cells and cells of Neuro-ectodermal origin. Differentiation. 73:474–483. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu L, Lu N, Liu X, Yi Q, Li S, Wen J, Sun H and Du Y: SBEM confers paclitaxel resistance in breast cancer via DUSP16‑mediated MAPK/AMPK pathway activation. Oncol Lett 30: 607, 2025.
APA
Liu, L., Lu, N., Liu, X., Yi, Q., Li, S., Wen, J. ... Du, Y. (2025). SBEM confers paclitaxel resistance in breast cancer via DUSP16‑mediated MAPK/AMPK pathway activation. Oncology Letters, 30, 607. https://doi.org/10.3892/ol.2025.15353
MLA
Liu, L., Lu, N., Liu, X., Yi, Q., Li, S., Wen, J., Sun, H., Du, Y."SBEM confers paclitaxel resistance in breast cancer via DUSP16‑mediated MAPK/AMPK pathway activation". Oncology Letters 30.6 (2025): 607.
Chicago
Liu, L., Lu, N., Liu, X., Yi, Q., Li, S., Wen, J., Sun, H., Du, Y."SBEM confers paclitaxel resistance in breast cancer via DUSP16‑mediated MAPK/AMPK pathway activation". Oncology Letters 30, no. 6 (2025): 607. https://doi.org/10.3892/ol.2025.15353
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Lu N, Liu X, Yi Q, Li S, Wen J, Sun H and Du Y: SBEM confers paclitaxel resistance in breast cancer via DUSP16‑mediated MAPK/AMPK pathway activation. Oncol Lett 30: 607, 2025.
APA
Liu, L., Lu, N., Liu, X., Yi, Q., Li, S., Wen, J. ... Du, Y. (2025). SBEM confers paclitaxel resistance in breast cancer via DUSP16‑mediated MAPK/AMPK pathway activation. Oncology Letters, 30, 607. https://doi.org/10.3892/ol.2025.15353
MLA
Liu, L., Lu, N., Liu, X., Yi, Q., Li, S., Wen, J., Sun, H., Du, Y."SBEM confers paclitaxel resistance in breast cancer via DUSP16‑mediated MAPK/AMPK pathway activation". Oncology Letters 30.6 (2025): 607.
Chicago
Liu, L., Lu, N., Liu, X., Yi, Q., Li, S., Wen, J., Sun, H., Du, Y."SBEM confers paclitaxel resistance in breast cancer via DUSP16‑mediated MAPK/AMPK pathway activation". Oncology Letters 30, no. 6 (2025): 607. https://doi.org/10.3892/ol.2025.15353
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team