Alterations of p14ARF and p16INK4a genes in salivary gland carcinomas

  • Authors:
    • Masayoshi Nishimine
    • Mitsutoshi Nakamura
    • Munehiro Kishi
    • Masumi Okamoto
    • Keiji Shimada
    • Eiwa Ishida
    • Tadaaki Kirita
    • Noboru Konishi
  • View Affiliations

  • Published online on: May 1, 2003     https://doi.org/10.3892/or.10.3.555
  • Pages: 555-560
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Abstract

The p14ARF and p16INK4a genes are localized to 9p21, where genetic alterations have been reported to be frequent in various human neoplasms. To elucidate their status in salivary gland tumorigenesis, we analyzed a series of 36 salivary gland carcinomas (SGCs) using methylation-specific PCR, differential PCR and immunohistochemistry. Homozygous deletion (3 cases) or methylation (7 cases) of p14ARF was detected in 10 (28%) SGCs, one and three showing co-deletion and co-methylation of both p14ARF and p16INK4a genes, respectively. A total of 5 (14%) SGCs demonstrated homozygous deletion (1 case) or methylation (4 cases) of p16INK4a, all but one being adenoid cystic carcinomas. Immunohistochemical study revealed loss of p14ARF and p16INK4a expression in 11 samples (31%), correlating with the gene status. These results indicate that inactivation of p14ARF and p16INK4a genes by either homozygous deletion or promoter hypermethylation may be important for the molecular pathogenesis of salivary malignant tumors, and provide clear evidence that epigenetic changes like methylation are related to salivary gland carcinogenesis.

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May-June 2003
Volume 10 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Nishimine M, Nakamura M, Kishi M, Okamoto M, Shimada K, Ishida E, Kirita T and Konishi N: Alterations of p14ARF and p16INK4a genes in salivary gland carcinomas. Oncol Rep 10: 555-560, 2003
APA
Nishimine, M., Nakamura, M., Kishi, M., Okamoto, M., Shimada, K., Ishida, E. ... Konishi, N. (2003). Alterations of p14ARF and p16INK4a genes in salivary gland carcinomas. Oncology Reports, 10, 555-560. https://doi.org/10.3892/or.10.3.555
MLA
Nishimine, M., Nakamura, M., Kishi, M., Okamoto, M., Shimada, K., Ishida, E., Kirita, T., Konishi, N."Alterations of p14ARF and p16INK4a genes in salivary gland carcinomas". Oncology Reports 10.3 (2003): 555-560.
Chicago
Nishimine, M., Nakamura, M., Kishi, M., Okamoto, M., Shimada, K., Ishida, E., Kirita, T., Konishi, N."Alterations of p14ARF and p16INK4a genes in salivary gland carcinomas". Oncology Reports 10, no. 3 (2003): 555-560. https://doi.org/10.3892/or.10.3.555