Phenoxazine derivatives induce caspase-independent cell death in human glioblastoma cell lines, A-172 and U-251 MG

  • Authors:
    • Ken Shirato
    • Kazuhiko Imaizumi
    • Akihisa Abe
    • Akio Tomoda
  • View Affiliations

  • Published online on: January 1, 2007     https://doi.org/10.3892/or.17.1.201
  • Pages: 201-208
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The apoptotic effects of 2-amino-4,4α-dihydro-4α, 7-dimethyl-3H-phenoxazine-3-one (Phx-1) and 2-aminophenoxazine-3-one (Phx-3) on human glioblastoma cell lines, A-172 and U-251 MG were studied. These phenoxazines extensively decreased the viability of A-172 and U-251 MG cells (IC50 of Phx-1: 60 µM, in both lines; IC50 of Phx-3: 10 and 3 µM, for A-172 and U-251 cells, respectively). Phx-1 and Phx-3 increased the population of annexin V and PI double-positive cells in A-172 and U-251 MG cells, resulting in cell death at late stage apoptosis/necrosis. The activities of caspase-3/7 were greatly increased in A-172 and U-251 MG cells treated with Phx-1 or Phx-3. However, a pan-caspase inhibitor, z-VAD-fmk, failed to reverse the antiproliferative and apoptotic effects of Phx-1 and Phx-3 in both cell lines. In conclusion, Phx-1 and Phx-3 exert significant anti-cancer effects against human glioblastoma cell lines, A-172 and U-251 MG, mediated by the caspase-independent apoptotic cell death pathway.

Related Articles

Journal Cover

January 2007
Volume 17 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Shirato K, Imaizumi K, Abe A and Tomoda A: Phenoxazine derivatives induce caspase-independent cell death in human glioblastoma cell lines, A-172 and U-251 MG. Oncol Rep 17: 201-208, 2007
APA
Shirato, K., Imaizumi, K., Abe, A., & Tomoda, A. (2007). Phenoxazine derivatives induce caspase-independent cell death in human glioblastoma cell lines, A-172 and U-251 MG. Oncology Reports, 17, 201-208. https://doi.org/10.3892/or.17.1.201
MLA
Shirato, K., Imaizumi, K., Abe, A., Tomoda, A."Phenoxazine derivatives induce caspase-independent cell death in human glioblastoma cell lines, A-172 and U-251 MG". Oncology Reports 17.1 (2007): 201-208.
Chicago
Shirato, K., Imaizumi, K., Abe, A., Tomoda, A."Phenoxazine derivatives induce caspase-independent cell death in human glioblastoma cell lines, A-172 and U-251 MG". Oncology Reports 17, no. 1 (2007): 201-208. https://doi.org/10.3892/or.17.1.201