Histone deacetylase inhibitors control the transcription and alternative splicing of prohibitin in thyroid tumor cells

  • Authors:
    • Cinzia Puppin
    • Nadia Passon
    • Alessandra Franzoni
    • Diego Russo
    • Giuseppe Damante
  • View Affiliations

  • Published online on: December 7, 2010     https://doi.org/10.3892/or.2010.1075
  • Pages: 393-397
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Abstract

Prohibitin (PHB) is a ubiquitous protein with a number of different molecular functions. PHB is involved in tumorigenesis by exerting either a permissive or blocking action on tumor growth, depending on the cell context. In the present study, we investigated the effects of the histone deacetylase inhibitors (HDACis), trichostatin A (TSA) and sodium butyrate (NaB), on PHB expression in the thyroid tumor cell lines, TPC-1 and FRO. Both TSA and NaB increased PHB mRNA levels. Transfection experiments showed that the overexpression of HDAC1 or 2, but not 3, inhibited PHB promoter activity. The effects of TSA and NaB on the two major PHB mRNA splicing isoforms, were also investigated. Both TSA and NaB decreased the mRNA levels of the shorter isoform, but increased those of the longer isoform. Only the latter isoform contains a 3'UTR, which has been reported to exert a growth suppressive action. Thus, our data demonstrate that HDACis control both PHB transcription and alternative splicing. The effect of HDACis on PHB alternative splicing was not due to the modification of the expression of the ASF/SF2 splicing factor.

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February 2011
Volume 25 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Puppin C, Passon N, Franzoni A, Russo D and Damante G: Histone deacetylase inhibitors control the transcription and alternative splicing of prohibitin in thyroid tumor cells. Oncol Rep 25: 393-397, 2011
APA
Puppin, C., Passon, N., Franzoni, A., Russo, D., & Damante, G. (2011). Histone deacetylase inhibitors control the transcription and alternative splicing of prohibitin in thyroid tumor cells. Oncology Reports, 25, 393-397. https://doi.org/10.3892/or.2010.1075
MLA
Puppin, C., Passon, N., Franzoni, A., Russo, D., Damante, G."Histone deacetylase inhibitors control the transcription and alternative splicing of prohibitin in thyroid tumor cells". Oncology Reports 25.2 (2011): 393-397.
Chicago
Puppin, C., Passon, N., Franzoni, A., Russo, D., Damante, G."Histone deacetylase inhibitors control the transcription and alternative splicing of prohibitin in thyroid tumor cells". Oncology Reports 25, no. 2 (2011): 393-397. https://doi.org/10.3892/or.2010.1075