UVC irradiation suppresses platelet-derived growth factor-BB-induced migration in human pancreatic cancer cells

Kawaguchi,Junji; Adachi,Seiji; Yasuda,Ichiro; Yamauchi,Takahiro; Yoshioka,Takashi; Itani,Masahiko; Kozawa,Osamu; Moriwaki,Hisataka

doi:10.3892/or.2011.1612

April 2012 Volume 27 Issue 4

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

April 2012 Volume 27 Issue 4

Full Size Image

Article Open Access

UVC irradiation suppresses platelet-derived growth factor-BB-induced migration in human pancreatic cancer cells

Authors:
- Junji Kawaguchi
- Seiji Adachi
- Ichiro Yasuda
- Takahiro Yamauchi
- Takashi Yoshioka
- Masahiko Itani
- Osamu Kozawa
- Hisataka Moriwaki
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan, Department of Gastroenterology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan

Copyright: © Kawaguchi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 935-939
|
Published online on: December 23, 2011

https://doi.org/10.3892/or.2011.1612
Expand metrics +

Abstract

We have recently reported that short wavelength ultraviolet-C (UVC) irradiation inhibits cell growth and induces apoptosis in human pancreatic cancer cells. In this study, we investigated the effect of UVC on platelet-derived growth factor (PDGF)-BB-induced migration in pancreatic cancer cells, AsPC1 and BxPC3. In cell migration assays using a Boyden chamber Transwell, PDGF-BB exerted a maximum effect on migration of these cells at a dose of 70 ng/ml after 36 h of treatment. PDGF-BB also caused phosphorylation of p44/p42 mitogen-activated protein kinase (MAPK), stress-activated protein kinase/c-Jun-N-terminal kinase (SAPK/JNK) and Akt, but not of p38 MAPK in these cells. Pretreatment of these cells with UVC at a dose over 10 J markedly suppressed PDGF-BB-induced migration. Since UVC significantly inhibited PDGF-BB-induced phosphorylation of Akt, and subsequent glycogen synthase kinase (GSK) 3β, but not p44/p42 MAPK and SAPK/JNK, it is likely that UVC inhibits PDGF-BB-induced migration by suppressing the Akt-GSK3β pathway in pancreatic cancer cells. Taken together with our previous findings, UVC could be a useful tool for the treatment of patients with pancreatic cancer.

View Figures

View References

1	Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar
2	Riento K and Ridley AJ: Rocks: multifunctional kinases in cell behaviour. Nat Rev Mol Cell Biol. 4:446–456. 2003. View Article : Google Scholar : PubMed/NCBI
3	Burridge K: Are stress fibres contractile? Nature. 294:691–692. 1981. View Article : Google Scholar : PubMed/NCBI
4	Humphries JD, Wang P, Streuli C, Geiger B, Humphries MJ and Ballestrem C: Vinculin controls focal adhesion formation by direct interactions with talin and actin. J Cell Biol. 179:1043–1057. 2007. View Article : Google Scholar : PubMed/NCBI
5	Burridge K and Chrzanowska-Wodnicka M: Focal adhesions, contractility, and signaling. Annu Rev Cell Dev Biol. 12:463–518. 1996. View Article : Google Scholar : PubMed/NCBI
6	Wang Z, Ahmad A, Li Y, et al: Emerging roles of PDGF-D signaling pathway in tumor development and progression. Biochim Biophys Acta. 1806:122–130. 2010.PubMed/NCBI
7	Henriksen R, Funa K, Wilander E, Backstrom T, Ridderheim M and Oberg K: Expression and prognostic significance of platelet-derived growth factor and its receptors in epithelial ovarian neoplasms. Cancer Res. 53:4550–4554. 1993.PubMed/NCBI
8	Uren A, Merchant MS, Sun CJ, et al: Beta-platelet-derived growth factor receptor mediates motility and growth of Ewing’s sarcoma cells. Oncogene. 22:2334–2342. 2003.PubMed/NCBI
9	Latonen L and Laiho M: Cellular UV damage responses-functions of tumor suppressor p53. Biochim Biophys Acta. 1755:71–89. 2005.PubMed/NCBI
10	Olsen BB, Neves-Petersen MT, Klitgaard S, Issinger OG and Petersen SB: UV light blocks EGFR signalling in human cancer cell lines. Int J Oncol. 30:181–185. 2007.PubMed/NCBI
11	Kim SC, Park SS and Lee YJ: Effect of UV irradiation on colorectal cancer cells with acquired TRAIL resistance. J Cell Biochem. 104:1172–1180. 2008. View Article : Google Scholar : PubMed/NCBI
12	Yamauchi T, Adachi S, Yasuda I, et al: UV-C irradiation induces downregulation of EGF receptor via phosphorylation at serine 1046/1047 in human pancreatic cancer cells. Radiat Res. 176:565–574. 2011. View Article : Google Scholar : PubMed/NCBI
13	Yamauchi T, Adachi S, Yasuda I, et al: Ultra-violet irradiation induces apoptosis via mitochondrial pathway in pancreatic cancer cells. Int J Oncol. 39:1375–1380. 2011.PubMed/NCBI
14	Adachi S, Yasuda I, Nakashima M, et al: Ultraviolet irradiation can induce evasion of colon cancer cells from stimulation of epidermal growth factor. J Biol Chem. 286:26178–26187. 2011. View Article : Google Scholar : PubMed/NCBI
15	Adachi S, Nagao T, Ingolfsson HI, et al: The inhibitory effect of (−)-epigallocatechin gallate on activation of the epidermal growth factor receptor is associated with altered lipid order in HT29 colon cancer cells. Cancer Res. 67:6493–6501. 2007.
16	Cross DA, Alessi DR, Cohen P, Andjelkovich M and Hemmings BA: Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature. 378:785–789. 1995. View Article : Google Scholar : PubMed/NCBI
17	Adachi S, Yasuda I, Nakashima M, et al: Rho-kinase inhibitor upregulates migration by altering focal adhesion formation via the Akt pathway in colon cancer cells. Eur J Pharmacol. 650:145–150. 2011. View Article : Google Scholar : PubMed/NCBI
18	Szodoray P, Papp G, Nakken B, Harangi M and Zeher M: The molecular and clinical rationale of extracorporeal photochemotherapy in autoimmune diseases, malignancies and transplantation. Autoimmun Rev. 9:459–464. 2010. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

UVC irradiation suppresses platelet-derived growth factor-BB-induced migration in human pancreatic cancer cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles