Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
April 2012 Volume 27 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April 2012 Volume 27 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus

  • Authors:
    • Han-Xian Gong
    • Lei He
    • Xiang-Pen Li
    • Yi-Dong Wang
    • Yi Li
    • Jun-Jian Huang
    • Ziling Wang
    • Dan Xie
    • Hsiang-Fu Kung
    • Ying Peng
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, P.R. China, Laboratory of Tumor and Molecular Biology, Beijing Institute of Biotechnology, Beijing, P.R. China, Institute of Blood Transfusion; Academy of Military Medical Sciences, Beijing, P.R. China, State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangzhou, P.R. China, Stanley Ho Center for Emerging Infectious Diseases, the Chinese University of Hong Kong, Shatin, Hong Kong SAR, P.R. China
  • Pages: 1163-1169
    |
    Published online on: December 30, 2011
       https://doi.org/10.3892/or.2011.1619
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

hTERTC27, a 27-kDa hTERT C-terminal polypeptide has been demonstrated to cause hTERT-positive HeLa cell apoptosis and inhibits the growth of mouse melanoma. hTERTC27 has been associated with telomere dysfunction, regulation of gene-regulated apoptosis, the cell cycle and activation of natural killer (NK) cells, but its mechanism of action is not fully understood. Here, we report that dendritic cells (DCs) transduced with hTERTC27 can increase T-cell proliferation, and augment the concentration of interleukin-2 (IL-2) and interferon-γ (IFN-γ) in the supernatants of T cells. It can also induce antigen-specific cytotoxic T lymphocytes (CTL) against glioma cells in vitro. Moreover, hTERTC27 gene-transduced DCs exhibit a very potent cytotoxicity to glioma cells in vivo. It could prolong the survival time and inhibit the growth of glioma-bearing mice. These data suggest that hTERTC27 gene-transduced DCs can efficiently enhance immunity against gliomas in vitro and in vivo.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Tanaka H, Shinto O, Yashiro M, et al: Transforming growth factor beta signaling inhibitor, SB-431542, induces maturation of dendritic cells and enhances anti-tumor activity. Oncol Rep. 24:1637–1643. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Kushwah R and Hu J: Complexity of dendritic cell subsets and their function in the host immune system. Immunology. 133:409–419. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Phuc PV, Lam DH, Ngoc VB, Thu DT, Nguyet NT and Ngoc PK: Production of functional dendritic cells from menstrual blood-a new dendritic cell source for immune therapy. In Vitro Cell Dev Biol Anim. 47:368–375. 2011.PubMed/NCBI

4 

Guermonprez P, Valladeau J, Zitvogel L, Thery C and Amigorena S: Antigen presentation and T cell stimulation by dendritic cells. Annu Rev Immunol. 20:621–667. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Pawlowska AB, Hashino S, McKenna H, Weigel BJ, Taylor PA and Blazar BR: In vitro tumor-pulsed or in vivo Flt3 ligand-generated dendritic cells provide protection against acute myelogenous leukemia in non-transplanted or syngeneic bone marrow-transplanted mice. Blood. 97:1474–1482. 2001. View Article : Google Scholar

6 

Onaitis M, Kalady MF, Pruitt S and Tyler DS: Dendritic cell gene therapy. Surg Oncol Clin N Am. 11:645–660. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Trivedi D, Williams RY, O'Reilly RJ and Koehne G: Generation of CMV-specific T lymphocytes using protein-spanning pools of pp65-derived overlapping pentadecapeptides for adoptive immunotherapy. Blood. 105:2793–2801. 2005. View Article : Google Scholar

8 

Bachleitner-Hofmann T, Friedl J, Hassler M, et al: Pilot trial of autologous dendritic cells loaded with tumor lysate(s) from allogeneic tumor cell lines in patients with metastatic medullary thyroid carcinoma. Oncol Rep. 21:1585–1592. 2009. View Article : Google Scholar

9 

Xiao ZY, Tang H, Xu ZM, et al: An experimental study of dendritic cells transfected with cancer stem-like cells RNA against 9L brain tumors. Cancer Biol Ther. 11:974–980. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Palucka AK, Ueno H, Connolly J, et al: Dendritic cells loaded with killed allogeneic melanoma cells can induce objective clinical responses and MART-1 specific CD8+ T-cell immunity. J Immunother. 29:545–557. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Sun L, Liu J, Cao X, et al: Enhancement of antigen presenting function of dendritic cells by IL-2 gene modification and its mechanism. Zhonghua Xue Ye Xue Za Zhi. 23:247–250. 2002.(In Chinese).

12 

Harrington L, Zhou W, McPhail T, et al: Human telomerase contains evolutionarily conserved catalytic and structural subunits. Genes Dev. 11:3109–3115. 1997. View Article : Google Scholar : PubMed/NCBI

13 

Collins K and Mitchell JR: Telomerase in the human organism. Oncogene. 21:564–579. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Shay JW, Zou Y, Hiyama E and Wright WE: Telomerase and cancer. Hum Mol Genet. 10:677–685. 2001. View Article : Google Scholar

15 

Zhang P, Chen Y, Jiang X, Tu Z and Zou L: Tumor-targeted efficiency of shRNA vector harboring chimera hTERT/U6 promoter. Oncol Rep. 23:1309–1316. 2010.PubMed/NCBI

16 

Li H, Wang J, Zhou T, Zhang Y and Zhang Z: An investigation of the effects of nanosize delivery system for antisense oligonucleotide on esophageal squamous cancer cells. Cancer Biol Ther. 7:1852–1859. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Keith WN, Bilsland A, Evans TR and Glasspool RM: Telomerase-directed molecular therapeutics. Expert Rev Mol Med. 4:1–25. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Vonderheide RH, Hahn WC, Schultze JL and Nadler LM: The telomerase catalytic subunit is a widely expressed tumor-associated antigen recognized by cytotoxic T lymphocytes. Immunity. 10:673–679. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Huang JJ, Lin MC, Bai YX, et al: Ectopic expression of a COOH-terminal fragment of the human telomerase reverse transcriptase leads to telomere dysfunction and reduction of growth and tumorigenicity in HeLa cells. Cancer Res. 62:3226–3232. 2002.

20 

Ng SS, Gao Y, Chau DH, et al: A novel glioblastoma cancer gene therapy using AAV-mediated long-term expression of human TERT C-terminal polypeptide. Cancer Gene Ther. 14:561–572. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Huo L, Yao H, Wang X, Wong GW, Kung HF and Lin MC: Inhibition of melanoma growth by subcutaneous administration of hTERTC27 viral cocktail in C57BL/6 mice. PLoS One. 5:e127052010. View Article : Google Scholar : PubMed/NCBI

22 

Yang JY, Cao DY, Liu WC, Zhang HM, Teng ZH and Ren J: Dendritic cell generated from CD34+ hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses. Cell Immunol. 240:14–21. 2006.PubMed/NCBI

23 

Chen T, Tang XD, Wan Y, et al: HLA-A2-restricted cytotoxic T lymphocyte epitopes from human heparanase as novel targets for broad-spectrum tumor immunotherapy. Neoplasia. 10:977–986. 2008.PubMed/NCBI

24 

Inaba K, Inaba M, Romani N, et al: Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J Exp Med. 176:1693–1702. 1992. View Article : Google Scholar

25 

Berzofsky JA, Terabe M, Oh S, et al: Progress on new vaccine strategies for the immunotherapy and prevention of cancer. J Clin Invest. 113:1515–1525. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Vonderheide RH, Domchek SM, Schultze JL, et al: Vaccination of cancer patients against telomerase induces functional antitumor CD8+ T lymphocytes. Clin Cancer Res. 10:828–839. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Nestle FO, Alijagic S, Gilliet M, et al: Vaccination of melanoma patients with peptide- or tumor lysate-pulsed dendritic cells. Nat Med. 4:328–332. 1998. View Article : Google Scholar : PubMed/NCBI

28 

Huo LF, Tang JW, Huang JJ, et al: Cancer immunotherapy targeting the telomerase reverse transcriptase. Cell Mol Immunol. 3:1–11. 2006.PubMed/NCBI

29 

Vonderheide RH, Anderson KS, Hahn WC, Butler MO, Schultze JL and Nadler LM: Characterization of HLA-A3-restricted cytotoxic T lymphocytes reactive against the widely expressed tumor antigen telomerase. Clin Cancer Res. 7:3343–3348. 2001.PubMed/NCBI

30 

Scardino A, Gross DA, Alves P, et al: HER-2/neu and hTERT cryptic epitopes as novel targets for broad spectrum tumor immunotherapy. J Immunol. 168:5900–5906. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Minev B, Hipp J, Firat H, Schmidt JD, Langlade-Demoyen P and Zanetti M: Cytotoxic T cell immunity against telomerase reverse transcriptase in humans. Proc Natl Acad Sci USA. 97:4796–4801. 2000. View Article : Google Scholar : PubMed/NCBI

32 

Rabinovich PM, Komarovskaya ME, Wrzesinski SH, et al: Chimeric receptor mRNA transfection as a tool to generate antineoplastic lymphocytes. Hum Gene Ther. 20:51–61. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Iuchi T, Teitz-Tennenbaum S, Huang J, et al: Interleukin-21 augments the efficacy of T-cell therapy by eliciting concurrent cellular and humoral responses. Cancer Res. 68:4431–4441. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Dredge K, Marriott JB, Todryk SM, et al: Protective antitumor immunity induced by a costimulatory thalidomide analog in conjunction with whole tumor cell vaccination is mediated by increased Th1-type immunity. J Immunol. 168:4914–4919. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Pletneva M, Fan H, Park JJ, et al: IFN-producing killer dendritic cells are antigen-presenting cells endowed with T-cell cross-priming capacity. Cancer Res. 69:6607–6614. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gong H, He L, Li X, Wang Y, Li Y, Huang J, Wang Z, Xie D, Kung H, Peng Y, Peng Y, et al: Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus. Oncol Rep 27: 1163-1169, 2012.
APA
Gong, H., He, L., Li, X., Wang, Y., Li, Y., Huang, J. ... Peng, Y. (2012). Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus. Oncology Reports, 27, 1163-1169. https://doi.org/10.3892/or.2011.1619
MLA
Gong, H., He, L., Li, X., Wang, Y., Li, Y., Huang, J., Wang, Z., Xie, D., Kung, H., Peng, Y."Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus". Oncology Reports 27.4 (2012): 1163-1169.
Chicago
Gong, H., He, L., Li, X., Wang, Y., Li, Y., Huang, J., Wang, Z., Xie, D., Kung, H., Peng, Y."Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus". Oncology Reports 27, no. 4 (2012): 1163-1169. https://doi.org/10.3892/or.2011.1619
Copy and paste a formatted citation
x
Spandidos Publications style
Gong H, He L, Li X, Wang Y, Li Y, Huang J, Wang Z, Xie D, Kung H, Peng Y, Peng Y, et al: Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus. Oncol Rep 27: 1163-1169, 2012.
APA
Gong, H., He, L., Li, X., Wang, Y., Li, Y., Huang, J. ... Peng, Y. (2012). Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus. Oncology Reports, 27, 1163-1169. https://doi.org/10.3892/or.2011.1619
MLA
Gong, H., He, L., Li, X., Wang, Y., Li, Y., Huang, J., Wang, Z., Xie, D., Kung, H., Peng, Y."Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus". Oncology Reports 27.4 (2012): 1163-1169.
Chicago
Gong, H., He, L., Li, X., Wang, Y., Li, Y., Huang, J., Wang, Z., Xie, D., Kung, H., Peng, Y."Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus". Oncology Reports 27, no. 4 (2012): 1163-1169. https://doi.org/10.3892/or.2011.1619
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team