Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
August 2012 Volume 28 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August 2012 Volume 28 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Regulated chemokine gene expression in mouse mesothelioma and mesothelial cells: TNF-α upregulates both CC and CXC chemokine genes

  • Authors:
    • Simon A. Fox
    • Suzanne S. Loh
    • Sathish K. Mahendran
    • Michael J. Garlepp
  • View Affiliations / Copyright

    Affiliations: Molecular Pharmacology Laboratory, School of Pharmacy, Western Australian Biomedical Research Institute and Curtin Health Innovation Research Institute, School of Pharmacy, Curtin University, Bentley, WA 6102, Australia
  • Pages: 707-713
    |
    Published online on: May 10, 2012
       https://doi.org/10.3892/or.2012.1809
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Many cancers express an array of chemokines which have the capacity to modulate the nature and function of intratumoural leukocyte infiltrates. In malignant mesothelioma (MM) neither the chemokine signalling networks nor their regulation have been investigated despite the prominence of leucocytic infiltrates in both clinical and experimental tumours. In this study, we examined constitutive and cytokine-regulated expression of CC and CXC chemokine genes in mesothelioma and mesothelial cell cultures derived from two different mouse strains (BALB/C and CBA/CaH). In mouse MM and mesothelial cells MCP-1/JE, GRO-α/KC and RANTES were expressed whereas MIP-1α and MIP-2 were infrequently expressed. Comparison of basal chemokine expression showed that GRO-α/KC mRNA was overexpressed in the malignant cells whereas MCP-1 gene expression and release was downregulated. Treatment of mesothelioma cells with IL-4, IFN-γ or TNF-α revealed that chemokine genes could be more responsive to cytokines in the malignant compared to their mesothelial cells. TNF-α was consistently the most potent positive regulator of both CC and CXC chemokine expression and MCP-1 release. The present study for the first time provides a mechanistic insight into the differential regulation of chemokine expression in malignant mesothelioma cells and has implications for mesothelial chemokine signalling in mouse models.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Robinson SC and Coussens LM: Soluble mediators of inflammation during tumor development. Adv Cancer Res. 93:159–187. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Mantovani A, Allavena P, Sozzani S, Vecchi A, Locati M and Sica A: Chemokines in the recruitment and shaping of the leukocyte infiltrate of tumors. Semin Cancer Biol. 14:155–160. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Balkwill F: Chemokine biology in cancer. Semin Immunol. 15:49–55. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Rollins BJ: Inflammatory chemokines in cancer growth and progression. Eur J Cancer. 42:760–767. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Strieter RM, Burdick MD, Mestas J, Gomperts B, Keane MP and Belperio JA: Cancer CXC chemokine networks and tumour angiogenesis. Eur J Cancer. 42:768–778. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Robinson BW, Musk AW and Lake RA: Malignant mesothelioma. Lancet. 366:397–408. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Davis MR, Manning LS, Whitaker D, Garlepp MJ and Robinson BW: Establishment of a murine model of malignant mesothelioma. Int J Cancer. 52:881–886. 1992. View Article : Google Scholar : PubMed/NCBI

8 

Fox SA, Loh S, Thean AL and Garlepp MJ: Identification of differentially expressed genes in murine mesothelioma cell lines of differing tumorigenicity using suppression subtractive hybridization. Biochim Biophys Acta. 1688:237–244. 2004. View Article : Google Scholar

9 

Leong CC, Marley JV, Loh S, Robinson BW and Garlepp MJ: The induction of immune responses to murine malignant mesothelioma by IL-2 gene transfer. Immunol Cell Biol. 75:356–359. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Bielefeldt-Ohmann H, Fitzpatrick DR, Marzo AL, et al: Patho- and immunobiology of malignant mesothelioma: characterisation of tumour infiltrating leucocytes and cytokine production in a murine model. Cancer Immunol Immunother. 39:347–359. 1994. View Article : Google Scholar

11 

Hegmans JP, Hemmes A, Aerts JG, Hoogsteden HC and Lambrecht BN: Immunotherapy of murine malignant mesothelioma using tumor lysate-pulsed dendritic cells. Am J Respir Crit Care Med. 171:1168–1177. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Li FK, Davenport A, Robson RL, et al: Leukocyte migration across human peritoneal mesothelial cells is dependent on directed chemokine secretion and ICAM-1 expression. Kidney Int. 54:2170–2183. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Mazar J, Agur T, Rogachev B, et al: CD40 ligand (CD154) takes part in regulation of the transition to mononuclear cell dominance during peritonitis. Kidney Int. 67:1340–1349. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Nasreen N, Mohammed KA, Hardwick J, et al: Polar production of interleukin-8 by mesothelial cells promotes the transmesothelial migration of neutrophils: role of intercellular adhesion molecule-1. J Infect Dis. 183:1638–1645. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Robson RL, McLoughlin RM, Witowski J, et al: Differential regulation of chemokine production in human peritoneal mesothelial cells: IFN-gamma controls neutrophil migration across the mesothelium in vitro and in vivo. J Immunol. 167:1028–1038. 2001. View Article : Google Scholar

16 

Mohammed KA, Nasreen N, Ward MJ and Antony VB: Helper T cell type 1 and 2 cytokines regulate C-C chemokine expression in mouse pleural mesothelial cells. Am J Respir Crit Care Med. 159:1653–1659. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Visser CE, Tekstra J, Brouwer-Steenbergen JJ, et al: Chemokines produced by mesothelial cells: huGRO-alpha, IP-10, MCP-1 and RANTES. Clin Exp Immunol. 112:270–275. 1998. View Article : Google Scholar : PubMed/NCBI

18 

Galffy G, Mohammed KA, Nasreen N, Ward MJ and Antony VB: Inhibition of interleukin-8 reduces human malignant pleural mesothelioma propagation in nude mouse model. Oncol Res. 11:187–194. 1999.PubMed/NCBI

19 

Galffy G, Mohammed KA, Dowling PA, Nasreen N, Ward MJ and Antony VB: Interleukin 8: an autocrine growth factor for malignant mesothelioma. Cancer Res. 59:367–371. 1999.PubMed/NCBI

20 

Robinson SC, Scott KA, Wilson JL, Thompson RG, Proudfoot AE and Balkwill FR: A chemokine receptor antagonist inhibits experimental breast tumor growth. Cancer Res. 63:8360–8365. 2003.PubMed/NCBI

21 

Bozic CR, Gerard NP, von Uexkull-Guldenband C, et al: The murine interleukin 8 type B receptor homologue and its ligands. Expression and biological characterization. J Biol Chem. 269:29355–29358. 1994.PubMed/NCBI

22 

Jackaman C, Bundell CS, Kinnear BF, et al: IL-2 intratumoral immunotherapy enhances CD8+ T cells that mediate destruction of tumor cells and tumor-associated vasculature: a novel mechanism for IL-2. J Immunol. 171:5051–5063. 2003.PubMed/NCBI

23 

Foley-Comer AJ, Herrick SE, Al-Mishlab T, Prele CM, Laurent GJ and Mutsaers SE: Evidence for incorporation of free-floating mesothelial cells as a mechanism of serosal healing. J Cell Sci. 115:1383–1389. 2002.PubMed/NCBI

24 

Rozen S and Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 132:365–386. 2000.PubMed/NCBI

25 

Pattyn F, Speleman F, De Paepe A and Vandesompele J: RTPrimerDB: the real-time PCR primer and probe database. Nucleic Acids Res. 31:122–123. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Vandesompele J, De Preter K, Pattyn F, et al: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3:Research00342002. View Article : Google Scholar : PubMed/NCBI

27 

Lin EY, Nguyen AV, Russell RG and Pollard JW: Colony-stimulating factor 1 promotes progression of mammary tumors to malignancy. J Exp Med. 193:727–740. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Nasreen N, Mohammed KA, Galffy G, Ward MJ and Antony VB: MCP-1 in pleural injury: CCR2 mediates haptotaxis of pleural mesothelial cells. Am J Physiol Lung Cell Mol Physiol. 278:L591–L598. 2000.PubMed/NCBI

29 

Davidson B, Dong HP, Holth A, Berner A and Risberg B: Chemokine receptors are infrequently expressed in malignant and benign mesothelial cells. Am J Clin Pathol. 127:752–759. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Loukinova E, Dong G, Enamorado-Ayalya I, et al: Growth regulated oncogene-alpha expression by murine squamous cell carcinoma promotes tumor growth, metastasis, leukocyte infiltration and angiogenesis by a host CXC receptor-2 dependent mechanism. Oncogene. 19:3477–3486. 2000. View Article : Google Scholar

31 

Zhou Y, Zhang J, Liu Q, et al: The chemokine GRO-alpha (CXCL1) confers increased tumorigenicity to glioma cells. Carcinogenesis. 26:2058–2068. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Hagemann T, Wilson J, Burke F, et al: Ovarian cancer cells polarize macrophages toward a tumor-associated phenotype. J Immunol. 176:5023–5032. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Szlosarek P, Charles KA and Balkwill FR: Tumour necrosis factor-alpha as a tumour promoter. Eur J Cancer. 42:745–750. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Fox SA, Kusmiaty, Loh SS, Dharmarajan AM and Garlepp MJ: Cisplatin and TNF-alpha downregulate transcription of Bcl-xL in murine malignant mesothelioma cells. Biochem Biophys Res Commun. 337:983–991. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Yao PL, Lin YC, Wang CH, et al: Autocrine and paracrine regulation of interleukin-8 expression in lung cancer cells. Am J Respir Cell Mol Biol. 32:540–547. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Charles PC, Weber KS, Cipriani B and Brosnan CF: Cytokine, chemokine and chemokine receptor mRNA expression in different strains of normal mice: implications for establishment of a Th1/Th2 bias. J Neuroimmunol. 100:64–73. 1999. View Article : Google Scholar : PubMed/NCBI

37 

Darville T, Andrews CW Jr, Sikes JD, Fraley PL, Braswell L and Rank RG: Mouse strain-dependent chemokine regulation of the genital tract T helper cell type 1 immune response. Infect Immun. 69:7419–7424. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Weinberg JB, Lutzke ML, Alfinito R and Rochford R: Mouse strain differences in the chemokine response to acute lung infection with a murine gammaherpesvirus. Viral Immunol. 17:69–77. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Fox SA, Loh SS, Mahendran SK and Garlepp MJ: Regulated chemokine gene expression in mouse mesothelioma and mesothelial cells: TNF-α upregulates both CC and CXC chemokine genes. Oncol Rep 28: 707-713, 2012.
APA
Fox, S.A., Loh, S.S., Mahendran, S.K., & Garlepp, M.J. (2012). Regulated chemokine gene expression in mouse mesothelioma and mesothelial cells: TNF-α upregulates both CC and CXC chemokine genes. Oncology Reports, 28, 707-713. https://doi.org/10.3892/or.2012.1809
MLA
Fox, S. A., Loh, S. S., Mahendran, S. K., Garlepp, M. J."Regulated chemokine gene expression in mouse mesothelioma and mesothelial cells: TNF-α upregulates both CC and CXC chemokine genes". Oncology Reports 28.2 (2012): 707-713.
Chicago
Fox, S. A., Loh, S. S., Mahendran, S. K., Garlepp, M. J."Regulated chemokine gene expression in mouse mesothelioma and mesothelial cells: TNF-α upregulates both CC and CXC chemokine genes". Oncology Reports 28, no. 2 (2012): 707-713. https://doi.org/10.3892/or.2012.1809
Copy and paste a formatted citation
x
Spandidos Publications style
Fox SA, Loh SS, Mahendran SK and Garlepp MJ: Regulated chemokine gene expression in mouse mesothelioma and mesothelial cells: TNF-α upregulates both CC and CXC chemokine genes. Oncol Rep 28: 707-713, 2012.
APA
Fox, S.A., Loh, S.S., Mahendran, S.K., & Garlepp, M.J. (2012). Regulated chemokine gene expression in mouse mesothelioma and mesothelial cells: TNF-α upregulates both CC and CXC chemokine genes. Oncology Reports, 28, 707-713. https://doi.org/10.3892/or.2012.1809
MLA
Fox, S. A., Loh, S. S., Mahendran, S. K., Garlepp, M. J."Regulated chemokine gene expression in mouse mesothelioma and mesothelial cells: TNF-α upregulates both CC and CXC chemokine genes". Oncology Reports 28.2 (2012): 707-713.
Chicago
Fox, S. A., Loh, S. S., Mahendran, S. K., Garlepp, M. J."Regulated chemokine gene expression in mouse mesothelioma and mesothelial cells: TNF-α upregulates both CC and CXC chemokine genes". Oncology Reports 28, no. 2 (2012): 707-713. https://doi.org/10.3892/or.2012.1809
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team