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Article

TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation

  • Authors:
    • Xiao Yan Ni
    • Hua Xiu Sui
    • Yao Liu
    • Shi Zhong Ke
    • Yi Nan Wang
    • Feng Guang Gao
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, Basic Medicine Science, Medical College, Xiamen University, Xiamen 361005, P.R. China
  • Pages: 615-621
    |
    Published online on: May 18, 2012
       https://doi.org/10.3892/or.2012.1822
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Abstract

The effects of TGF-β on dendritic cells (DCs) on the tumor microenvironment are not well understood. We report, here, the establishment of an in vitro lung cancer microenvironment by co-incubation of seminaphtharhodafluor (SNARF) labeled Lewis lung cancer (LLC) cells, carboxyfluorescein succinimidyl ester (CFSE) labeled fibroblasts and 4-chloromethyl-7-hydroxycoumarin (CMHC) labeled DCs. Raw 264.7, EL4 and NCI-H446 cells were able to synthesize TGF-β which was determined by flow cyto­metry and western blotting, respectively. Furthermore, TGF-β efficiently increased regulatory T-cell (Treg) expansion and upregulated DC B7H1 and GITRL expression. TGF-β and the co-incubation of LLC cells, fibroblasts with DCs could augment the expression of B7H1 and GITRL molecules of DCs. The data presented here indicate that the B7H1 and GITRL molecules may play an important role in TGF-β-induced Treg expansion of lung cancer microenvironment.
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1 

Witz IP: The tumor microenvironment: the making of a paradigm. Cancer Microenviron. 2(Suppl 1): 9–17. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Mueller MM and Fusenig NE: Friends or foes - bipolar effects of the tumor stroma in cancer. Nat Rev Cancer. 4:839–849. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Barsky SH, Green WR, Grotendorst GR and Liotta LA: Desmoplastic breast carcinoma as a source of human myofibroblasts. Am J Pathol. 115:329–333. 1984.PubMed/NCBI

4 

Kalluri R and Zeisberg M: Fibroblasts in cancer. Nat Rev Cancer. 6:392–401. 2006. View Article : Google Scholar

5 

Zou WP: Immunosuppressive networks in the tumor environment and their therapeutic relevance. Nat Rev Cancer. 5:263–274. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Bergmann C, Strauss L, Zeidler R, Lang S and Whiteside TL: Expansion and characteristics of human T regulatory type 1 cells in co-cultures simulating tumor microenvironment. Cancer Immunol Immunother. 56:1429–1442. 2007. View Article : Google Scholar

7 

Zou WP, Machelon V, Coulomb-L’Hermin A, et al: Stromal-derived factor-1 in human tumors recruits and alters the function of plasmacytoid precursor dendritic cells. Nat Med. 7:1339–1346. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Iwamoto M, Shinohara H, Miyamoto A, et al: Prognostic value of tumor-infiltrating dendritic cells expressing CD83 in human breast carcinomas. Int J Cancer. 104:92–97. 2003. View Article : Google Scholar

9 

Gao F, Hui X, He X, Wan D and Gu J: Dysfunction of murine dendritic cells induced by incubation with tumor cells. Cell Mol Immunol. 5:133–140. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Yuan XL, Chen L, Zhang TT, et al: Gastric cancer cells induce human CD4+Foxp3+ regulatory T cells through the production of TGF-β1. World J Gastroenterol. 17:2019–2027. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Sempere LF, Gunn JR and Korc M: A novel 3-dimensional culture system uncovers growth stimulatory actions by TGFβ in pancreatic cancer cells. Cancer Biol Ther. 12:198–207. 2011.PubMed/NCBI

12 

Yi T, Li X, Yao S, et al: Host APCs augment in vivo expansion of donor natural regulatory T cells via B7H1/B7.1 in allogeneic recipients. J Immunol. 186:2739–2749. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Zhou X, Zhou Y, Ding Q, et al: High level expression of B7H1 molecules by keratinocytes suppresses xeno- and allo-reactions by inducing type I regulatory T cells. Transpl Immunol. 21:192–197. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Hori J, Taniguchi H, Wang M, Oshima M and Azuma M: GITR ligand-mediated local expansion of regulatory T cells and immune privilege of corneal allografts. Invest Ophthalmol Vis Sci. 51:6556–6565. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Kim JI, Sonawane SB, Lee MK, et al: Blockade of GITR-GITRL interaction maintains Treg function to prolong allograft survival. Eur J Immunol. 40:1369–1374. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Häkkinen L, Koivisto L and Larjava H: An improved method for culture of epidermal keratinocytes from newborn mouse skin. Methods Cell Sci. 23:189–196. 2011.PubMed/NCBI

17 

Gao FG, Li HT, Li ZJ and Gu JR: Nicotine stimulated dendritic cells could achieve anti-tumor effects in mouse lung and liver cancer. J Clin Immunol. 31:80–88. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Zhang MH, Tang H, Guo ZH, et al: Splenic stroma drives mature dendritic cells to differentiate into regulatory dendritic cells. Nat Immunol. 5:1124–1133. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Ruan Q, Kameswaran V, Tone Y, et al: Development of Foxp3(+) regulatory T cells is driven by the c-Rel enhanceosome. Immunity. 31:932–940. 2009.

20 

Massague J: TGF-beta and cancer. Cell. 134:215–230. 2008. View Article : Google Scholar

21 

Alshaker HA and Matalka KZ: IFN-γ, IL-17 and TGF-β involvement in shaping the tumor microenvironment: the significance of modulating such cytokines in treating malignant solid tumors. Cancer Cell Int. 11:332011.

22 

Yang L, Pang Y and Moses HL: TGF-β and immune cells: an important regulatory axis in the tumor microenvironment and progression. Trends Immunol. 31:220–227. 2010.

23 

Siegel PM and Massague J: Cytostatic and apoptotic actions of TGF-β in homeostasis and cancer. Nat Rev Cancer. 3:807–821. 2003.

24 

Orsini E, Guarini A, Chiaretti S, Mauro FR and Foa R: The circulating dendritic cell compartment in patients with chronic lymphocytic leukemia is severely defective and unable to stimulate an effective T-cell response. Cancer Res. 63:4497–4506. 2003.

25 

Sharma S, Stolina M, Yang SC, et al: Tumor cyclooxygenase 2-dependent suppression of dendritic cell function. Clin Cancer Res. 9:961–968. 2003.PubMed/NCBI

26 

Yang AS and Lattime EC: Tumor-induced interleukin 10 suppresses the ability of splenic dendritic cell to stimulate CD4 and CD8 T-cell responses. Cancer Res. 63:2150–2157. 2003.PubMed/NCBI

27 

Nam JS, Terabe M, Kang MJ, et al: Transforming growth factor beta subverts the immune system into directly promoting tumor growth through interleukin-17. Cancer Res. 68:3915–3923. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Gorska AE, Jensen RA, Shyr Y, Aakre ME, Bhowmick NA and Moses HL: Transgenic mice expressing a dominant-negative mutant type II transforming growth factor beta receptor exhibit impaired mammary development and enhanced mammary tumor formation. Am J Pathol. 163:1539–1549. 2003. View Article : Google Scholar

29 

Scholzen A, Mittag D, Rogerson SJ, Cooke BM and Plebanski M: Plasmodium falciparum-mediated induction of human CD25hiFoxp3hi CD4 T cells is independent of direct TCR stimulation and requires IL-2, IL-10 and TGFβ. PLoS Pathog. 5:e10005432009.PubMed/NCBI

30 

Bettelli E, Carrier Y, Gao W, et al: Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells. Nature. 441:235–238. 2006. View Article : Google Scholar

31 

Kretschmer K, Apostolou I, Hawiger D, Khazaie K, Nussenzweig MC and von Boehmer H: Inducing and expanding regulatory T cell populations by foreign antigen. Nat Immunol. 6:1219–1227. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Lafaille C, Kutchukhidze MA, Shen N, Ding S, Yee YH and Lafaille JJ: Adaptive Foxp3+ regulatory T cell-dependent and -independent control of allergic inflammation. Immunity. 29:114–126. 2008.

33 

Yamazaki S, Iyoda T, Tarbell K, Olson K, Velinzon K, Inaba K and Steinman RM: Direct expansion of functional CD25+ CD4+ regulatory T cells by antigen-processing dendritic cells. J Exp Med. 198:235–247. 2003.PubMed/NCBI

34 

Benson MJ, Pino-Lagos K, Rosemblatt M and Noelle RJ: All-trans retinoic acid mediates enhanced T reg cell growth, differentiation, and gut homing in the face of high levels of co-stimulation. J Exp Med. 204:1765–1774. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ni XY, Sui HX, Liu Y, Ke SZ, Wang YN and Gao FG: TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation. Oncol Rep 28: 615-621, 2012.
APA
Ni, X.Y., Sui, H.X., Liu, Y., Ke, S.Z., Wang, Y.N., & Gao, F.G. (2012). TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation. Oncology Reports, 28, 615-621. https://doi.org/10.3892/or.2012.1822
MLA
Ni, X. Y., Sui, H. X., Liu, Y., Ke, S. Z., Wang, Y. N., Gao, F. G."TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation". Oncology Reports 28.2 (2012): 615-621.
Chicago
Ni, X. Y., Sui, H. X., Liu, Y., Ke, S. Z., Wang, Y. N., Gao, F. G."TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation". Oncology Reports 28, no. 2 (2012): 615-621. https://doi.org/10.3892/or.2012.1822
Copy and paste a formatted citation
x
Spandidos Publications style
Ni XY, Sui HX, Liu Y, Ke SZ, Wang YN and Gao FG: TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation. Oncol Rep 28: 615-621, 2012.
APA
Ni, X.Y., Sui, H.X., Liu, Y., Ke, S.Z., Wang, Y.N., & Gao, F.G. (2012). TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation. Oncology Reports, 28, 615-621. https://doi.org/10.3892/or.2012.1822
MLA
Ni, X. Y., Sui, H. X., Liu, Y., Ke, S. Z., Wang, Y. N., Gao, F. G."TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation". Oncology Reports 28.2 (2012): 615-621.
Chicago
Ni, X. Y., Sui, H. X., Liu, Y., Ke, S. Z., Wang, Y. N., Gao, F. G."TGF-β of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation". Oncology Reports 28, no. 2 (2012): 615-621. https://doi.org/10.3892/or.2012.1822
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