Suppression of growth of A549 lung cancer cells by waltonitone and its mechanisms of action

Zhang,Yi; Zhang,Guo-Bin; Xu,Xiao-Man; Zhang,Meng; Qu,Dan; Niu,Hui-Yan; Bai,Xue; Kan,Liang; He,Ping

doi:10.3892/or.2012.1869

Suppression of growth of A549 lung cancer cells by waltonitone and its mechanisms of action

Authors:
- Yi Zhang
- Guo-Bin Zhang
- Xiao-Man Xu
- Meng Zhang
- Dan Qu
- Hui-Yan Niu
- Xue Bai
- Liang Kan
- Ping He
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Affiliations: Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang 110004, P.R. China, Shenyang Center for Disease Control and Prevention, Shenyang 110004, P.R. China, Department of Respiratory Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, P.R. China, Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang 110004, P.R. Chinav
Published online on: June 15, 2012 https://doi.org/10.3892/or.2012.1869
Pages: 1029-1035

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Abstract

Natural compounds are a great source of cancer chemotherapeutic agents. Our investigation indicates that waltonitone, a triterpene extracted from medicinal plants, inhibits the proliferation of A549 cells in a concentration- and time-dependent manner. Waltonitone induced apoptosis of A549 cells in a concentration-dependent manner, as determined by fluorescence microscopy and flow cytometry. The apoptosis was accompanied by increased Bax protein levels and decreased Bcl-2 protein levels in A549 cells. Furthermore, the treatment of A549 cells with waltonitone altered the expression of miRNAs. We found that 27 miRNAs were differentially expressed in waltonitone-treated cells, of which 8 miRNAs target genes related to cell proliferation and apoptosis. In summary, our results demonstrate that waltonitone has a significant inhibitory effect on the proliferation of A549 cells. It is possible that upregulation of Bax/Bcl-2 and regulation of expression of specific miRNAs play a role in inhibition of proliferation and induction of apoptosis in waltonitone-treated cells. Waltonitone can be applied to lung carcinoma as a chemotherapeutic candidate.

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