Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
September 2012 Volume 28 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September 2012 Volume 28 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach

  • Authors:
    • Yoshiyuki Mizushina
    • Takahiko Hada
    • Hiromi Yoshida
  • View Affiliations / Copyright

    Affiliations: Department of Nutritional Science, Laboratory of Food and Nutritional Sciences, Nishi-ku, Kobe, Hyogo 651-2180, Japan, Hada Giken Co., Ltd., Yamaguchi-shi, Yamaguchi 753-0047, Japan
  • Pages: 821-828
    |
    Published online on: July 3, 2012
       https://doi.org/10.3892/or.2012.1893
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The glycoglycerolipid monogalactosyl diacylglycerol (MGDG) isolated from spinach selectively inhibits the activities of replicative DNA polymerase species and suppresses the growth of human cancer cell lines, while not affecting normal human cells. Liposomes, carrying surface-bound sialyl Lewis X (SLX) and containing MGDG (SLX-Lipo-MGDG) and the fluorescent dye Cy5.5, were administered intravenously to mice bearing HT-29 human colon adenocarcinoma tumors and liposome distribution observed using fluorescence imaging equipment in vivo. In an in vivo antitumor assay on nude mice bearing HT-29 solid tumors, SLX-Lipo-MGDG was shown to be a stronger and more promising suppressor of solid tumors than MGDG alone. These results suggest that spinach MGDG could be developed into an anticancer compound, SLX-Lipo-MGDG could serve as an effective clinical anticancer drug and that these liposomes may be useful tools as the basis for active targeting drug delivery systems.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Terry P, Giovannucci E, Michels KB, Bergkvist L, Hansen H, Holmberg L and Wolk A: Fruit, vegetables, dietary fiber, and risk of colorectal cancer. J Natl Cancer Inst. 93:525–533. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Surh YJ: Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer. 3:768–780. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Liu RH: Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr. 134:S3479–S3485. 2004.PubMed/NCBI

4 

Kornberg A and Baker TA: DNA replication. W.D. Freeman and Co; New York, NY: Chapter 6. 2nd edit. pp. 197–225. 1992

5 

DePamphilis ML: DNA replication in eukaryotic cells. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY: 1996

6 

Hübscher U, Maga G and Spadari S: Eukaryotic DNA polymerases. Annu Rev Biochem. 71:133–163. 2002.

7 

Bebenek K and Kunkel TA: Functions of DNA polymerases. Adv Protein Chem. 69:137–165. 2004. View Article : Google Scholar

8 

Takata K, Shimizu T, Iwai S and Wood RD: Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol. J Biol Chem. 281:23445–23455. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Loeb LA and Monnat RJ Jr: DNA polymerases and human disease. Nat Rev Genet. 9:594–604. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Mizushina Y, Watanabe I, Ohta K, Takemura M, Sahara H, Takahashi N, Gasa S, Sugawara F, Matsukage A, Yoshida S and Sakaguchi K: Studies on inhibitors of mammalian DNA polymerase α and β: sulfolipids from a pteridophyte, Athyrium niponicum. Biochem Pharmacol. 55:537–541. 1998.

11 

Ohta K, Mizushina Y, Hirata N, Takemura M, Sugawara F, Matsukage A, Yoshida S and Sakaguchi K: Sulfoquinovosyldiacylglycerol, KM043, a new potent inhibitor of eukaryotic DNA polymerases and HIV-reverse transcriptase type 1 from a marine red alga, Gigartina tenella. Chem Pharm Bull (Tokyo). 46:684–686. 1998. View Article : Google Scholar : PubMed/NCBI

12 

Roughan PG and Batt RD: The glycerolipid composition of leaves. Phytochemistry. 8:363–369. 1969. View Article : Google Scholar

13 

Sugawara T and Miyazawa T: Separation and determination of glycolipids from edible plant sources by high-performance liquid chromatography and evaporative light-scattering detection. Lipids. 34:1231–1237. 1999.PubMed/NCBI

14 

Yunoki K, Sato M, Seki K, Ohkubo T, Tanaka Y and Ohnishi M: Simultaneous quantification of plant glyceroglycolipids including sulfoquinovosyldiacylglycerol by HPLC-ELSD with binary gradient elution. Lipids. 44:77–83. 2009. View Article : Google Scholar

15 

Kuriyama I, Musumi K, Yonezawa Y, Takemura M, Maeda N, Iijima H, Hada T, Yoshida H and Mizushina Y: Inhibitory effects of glycolipids fraction from spinach on mammalian DNA polymerase activity and human cancer cell proliferation. J Nutr Biochem. 16:594–601. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Gabison A, Shmeeda H and Barenholz Y: Pharmacokinetics of pegylated liposomal Doxorubicin: review of animal and human studies. Clin Pharmacokinet. 42:419–436. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Unezaki S, Maruyama K, Ishida O, Takahashi N and Iwatsuru M: Enhanced tumor targeting of Doxorubicin by ganglioside GM1-bearing long-circulating liposomes. J Drug Target. 1:287–292. 1993. View Article : Google Scholar : PubMed/NCBI

18 

Maruyama K, Yuda T, Okamoto A, Kojima S, Suginaka A and Iwatsuru M: Prolonged circulation time in vivo of large unilamellar liposomes composed of distearoyl phosphatidylcholine and cholesterol containing amphipathic poly (ethylene glycol). Biochim Biophys Acta. 1128:44–49. 1992.

19 

Gabizon A and Papahadjopoulos D: Liposome formulation with prolonged circulation time in blood and enhanced uptake by tumors. Proc Natl Acad Sci USA. 85:6949–6953. 1988. View Article : Google Scholar : PubMed/NCBI

20 

Vyas SP, Singh A and Sihorkar V: Ligand-receptor-mediated drug delivery: an emerging paradigm in cellular drug targeting. Crit Rev Ther Drug Carrier Syst. 18:1–76. 2001.PubMed/NCBI

21 

Willis M and Forssen E: Ligand-targeted liposomes. Adv Drug Deliv Rev. 29:249–271. 1998. View Article : Google Scholar

22 

Yamazaki N, Kojima S and Yokoyama H: Biomedical nanotechnology for active drug delivery systems by applying sugar-chain molecular functions. Curr Appl Phys. 5:112–117. 2005. View Article : Google Scholar

23 

Yamazaki N: Active targeting DDS. Farumashia. 42:125–129. 2006.

24 

Ehrhardt C, Kneuer C and Bakowsky U: Selectin-an emerging target for drug delivery. Adv Drug Deliv Rev. 56:527–549. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Dodd RB and Drickamer K: Lectin-like proteins in model organisms: implications for evolution of carbohydrate-binding activity. Glycobiology. 11:R71–R79. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Kilpatrick DC: Animal lectins: a historical introduction and overview. Biochim Biophys Acta. 1572:187–197. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Bevilacqua MP, Stengelin S, Gimbrone MA Jr and Seed B: Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science. 243:1160–1165. 1989. View Article : Google Scholar : PubMed/NCBI

28 

Vestweber D and Blanks JE: Mechanisms that regulate the function of the selectins and their ligands. Physiol Rev. 79:181–213. 1999.PubMed/NCBI

29 

Hirai M, Minematsu H, Kondo N, Oie K, Igarashi K and Yamazaki N: Accumulation of liposome with Sialyl Lewis X to inflammation and tumor region: application to in vivo bio-imaging. Biochem Biophys Res Commun. 353:553–558. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Myobatake Y, Takeuchi T, Kuramochi K, Kuriyama I, Ishido T, Hirano K, Sugawara F, Yoshida H and Mizushina Y: Pinophilins A and B, inhibitors of mammalian A-, B-, and Y-family DNA polymerases and human cancer cell proliferation. J Nat Prod. 75:135–141. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Mizushina Y, Tanaka N, Yagi H, Kurosawa T, Onoue M, Seto H, Horie T, Aoyagi N, Yamaoka M, Matsukage A, et al: Fatty acids selectively inhibit eukaryotic DNA polymerase activities in vitro. Biochim Biophys Acta. 1308:256–262. 1996. View Article : Google Scholar : PubMed/NCBI

32 

Mizushina Y, Yoshida S, Matsukage A and Sakaguchi K: The inhibitory action of fatty acids on DNA polymerase β. Biochim Biophys Acta. 1336:509–521. 1997.

33 

Umeda S, Muta T, Ohsato T, Takamatsu C, Hamasaki N and Kang D: The D-loop structure of human mtDNA is destabilized directly by 1-methyl-4-phenylpyridinium ion (MPP+), a parkinsonism-causing toxin. Eur J Biochem. 267:200–206. 2000.PubMed/NCBI

34 

Ogawa A, Murate T, Suzuki M, Nimura Y and Yoshida S: Lithocholic acid, a putative tumor promoter, inhibits mammalian DNA polymerase β. Jpn J Cancer Res. 89:1154–1159. 1998.PubMed/NCBI

35 

Tamiya-Koizumi K, Murate T, Suzuki M, Simbulan CG, Nakagawa M, Takamura M, Furuta K, Izuta S and Yoshida S: Inhibition of DNA primase by sphingosine and its analogues parallels with their growth suppression of cultured human leukemic cells. Biochem Mol Biol Int. 41:1179–1189. 1997.PubMed/NCBI

36 

Nakayama C and Saneyoshi M: Inhibitory effects of 9-β-D-xylofuranosyladenine 5′-triphosphate on DNA-dependent RNA polymerase I and II from cherry salmon (Oncorhynchus masou). J Biochem. 97:1385–1389. 1985.

37 

Soltis DA and Uhlenbeck OC: Isolation and characterization of two mutant forms of T4 polynucleotide kinase. J Biol Chem. 257:11332–11339. 1982.PubMed/NCBI

38 

Lu BC and Sakaguchi K: An endo-exonuclease from meiotic tissues of the basidiomycete Coprinus cinereus: its purification and characterization. J Biol Chem. 266:21060–21066. 1991.PubMed/NCBI

39 

Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 65:55–63. 1983. View Article : Google Scholar : PubMed/NCBI

40 

Welply JK, Abbas SZ, Scudder P, Keene JL, Broschat K, Casnocha S, Gorka C, Steininger C, Howard SC and Schmuke JJ: Multivalent sialyl-LeX: potent inhibitors of E-selectin-mediated cell adhesion; reagent for staining activated endothelial cells. Glycobiology. 4:259–265. 1994. View Article : Google Scholar : PubMed/NCBI

41 

Pober JS, Bevilacqua MP, Mendrick DL, Lapierre LA, Fiers W and Gimbrone MA Jr: Two distinct monokines, interleukin 1 and tumor necrosis factor, each independently induce biosynthesis and transient expression of the same antigen on the surface of cultured human vascular endothelial cells. J Immunol. 136:1680–1687. 1986.

42 

Sugawara T and Miyazawa T: Digestion of plant monogalactosyldiacylglycerol and digalactosyldiacylglycerol in rat alimentary canal. J Nutr Biochem. 11:147–152. 2000. View Article : Google Scholar : PubMed/NCBI

43 

Drummond DC, Meyer O, Hong K, Kirpotin DB and Papahadjopoulos D: Optimizing liposome for delivery of chemotherapeutic agents to solid tumors. Pharmacol Rev. 51:691–743. 1999.PubMed/NCBI

44 

Yamazaki N, Kodama M and Gabius HJ: Neoglycoprotein–liposome and lectin-liposome conjugates as tools for carbohydrate recognition research. Methods Enzymol. 242:56–65. 1994.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Mizushina Y, Hada T and Yoshida H: In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach. Oncol Rep 28: 821-828, 2012.
APA
Mizushina, Y., Hada, T., & Yoshida, H. (2012). In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach. Oncology Reports, 28, 821-828. https://doi.org/10.3892/or.2012.1893
MLA
Mizushina, Y., Hada, T., Yoshida, H."In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach". Oncology Reports 28.3 (2012): 821-828.
Chicago
Mizushina, Y., Hada, T., Yoshida, H."In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach". Oncology Reports 28, no. 3 (2012): 821-828. https://doi.org/10.3892/or.2012.1893
Copy and paste a formatted citation
x
Spandidos Publications style
Mizushina Y, Hada T and Yoshida H: In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach. Oncol Rep 28: 821-828, 2012.
APA
Mizushina, Y., Hada, T., & Yoshida, H. (2012). In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach. Oncology Reports, 28, 821-828. https://doi.org/10.3892/or.2012.1893
MLA
Mizushina, Y., Hada, T., Yoshida, H."In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach". Oncology Reports 28.3 (2012): 821-828.
Chicago
Mizushina, Y., Hada, T., Yoshida, H."In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach". Oncology Reports 28, no. 3 (2012): 821-828. https://doi.org/10.3892/or.2012.1893
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team