In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach

Mizushina,Yoshiyuki; Hada,Takahiko; Yoshida,Hiromi

doi:10.3892/or.2012.1893

In vivo antitumor effect of liposomes with sialyl Lewis X including monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach

Authors:
- Yoshiyuki Mizushina
- Takahiko Hada
- Hiromi Yoshida
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Affiliations: Department of Nutritional Science, Laboratory of Food and Nutritional Sciences, Nishi-ku, Kobe, Hyogo 651-2180, Japan, Hada Giken Co., Ltd., Yamaguchi-shi, Yamaguchi 753-0047, Japan
Published online on: July 3, 2012 https://doi.org/10.3892/or.2012.1893
Pages: 821-828

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Abstract

The glycoglycerolipid monogalactosyl diacylglycerol (MGDG) isolated from spinach selectively inhibits the activities of replicative DNA polymerase species and suppresses the growth of human cancer cell lines, while not affecting normal human cells. Liposomes, carrying surface-bound sialyl Lewis X (SLX) and containing MGDG (SLX-Lipo-MGDG) and the fluorescent dye Cy5.5, were administered intravenously to mice bearing HT-29 human colon adenocarcinoma tumors and liposome distribution observed using fluorescence imaging equipment in vivo. In an in vivo antitumor assay on nude mice bearing HT-29 solid tumors, SLX-Lipo-MGDG was shown to be a stronger and more promising suppressor of solid tumors than MGDG alone. These results suggest that spinach MGDG could be developed into an anticancer compound, SLX-Lipo-MGDG could serve as an effective clinical anticancer drug and that these liposomes may be useful tools as the basis for active targeting drug delivery systems.

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