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Article

Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer

  • Authors:
    • Wei-Shone Chen
    • Chung-Man Leung
    • Hung-Wei Pan
    • Ling-Yueh Hu
    • Sung-Chou Li
    • Meng-Ru Ho
    • Kuo-Wang Tsai
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Veterans General Hospital, Taipei, Taiwan, R.O.C., Department of Radiation Oncology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C., Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C., Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, R.O.C., Genomics Research Center, Academia Sinica, Taipei, Taiwan, R.O.C.
  • Pages: 1069-1076
    |
    Published online on: July 5, 2012
       https://doi.org/10.3892/or.2012.1899
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Abstract

MicroRNAs are small non-coding RNA molecules that play important roles in the multistep process of colorectal carcinoma (CRC) development. The present study evaluated the relationship between miR-1-1 and miR-133a-2 expression and DNA methylation, and its putative biological role in CRC. The results indicated that DNA methylation regulated the expression of the miR-1-1 and miR-133a-2 cluster in CRC cell lines. Expression of miR-1 and miR-133a was further evaluated in 64 paired tissue samples (CRC tumor and adjacent normal mucosa) using the stem-loop real-time polymerase chain reaction. The miR-1-133a cluster displayed significantly lower expression in CRC tissue compared to adjacent normal mucosa (P<0.001). The results also indicated frequent hypermethylation of the CpG islands upstream of miR-1-133a (54.6%). Liver metastatic tissues exhibited significantly lower miR-1 (P<0.001) and miR-133a (P<0.001) expression compared to adjacent normal mucosa. Expression of the miR-1-133a cluster inversely correlated with TAGLN2 in the tumor specimens. In conclusion, epigenetic repression of the miR-1-133a cluster may play a critical role in colorectal cancer metastasis by silencing TAGLN2.
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Copy and paste a formatted citation
Spandidos Publications style
Chen W, Leung C, Pan H, Hu L, Li S, Ho M and Tsai K: Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer. Oncol Rep 28: 1069-1076, 2012.
APA
Chen, W., Leung, C., Pan, H., Hu, L., Li, S., Ho, M., & Tsai, K. (2012). Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer. Oncology Reports, 28, 1069-1076. https://doi.org/10.3892/or.2012.1899
MLA
Chen, W., Leung, C., Pan, H., Hu, L., Li, S., Ho, M., Tsai, K."Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer". Oncology Reports 28.3 (2012): 1069-1076.
Chicago
Chen, W., Leung, C., Pan, H., Hu, L., Li, S., Ho, M., Tsai, K."Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer". Oncology Reports 28, no. 3 (2012): 1069-1076. https://doi.org/10.3892/or.2012.1899
Copy and paste a formatted citation
x
Spandidos Publications style
Chen W, Leung C, Pan H, Hu L, Li S, Ho M and Tsai K: Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer. Oncol Rep 28: 1069-1076, 2012.
APA
Chen, W., Leung, C., Pan, H., Hu, L., Li, S., Ho, M., & Tsai, K. (2012). Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer. Oncology Reports, 28, 1069-1076. https://doi.org/10.3892/or.2012.1899
MLA
Chen, W., Leung, C., Pan, H., Hu, L., Li, S., Ho, M., Tsai, K."Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer". Oncology Reports 28.3 (2012): 1069-1076.
Chicago
Chen, W., Leung, C., Pan, H., Hu, L., Li, S., Ho, M., Tsai, K."Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer". Oncology Reports 28, no. 3 (2012): 1069-1076. https://doi.org/10.3892/or.2012.1899
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