Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer

  • Authors:
    • Wei-Shone Chen
    • Chung-Man Leung
    • Hung-Wei Pan
    • Ling-Yueh Hu
    • Sung-Chou Li
    • Meng-Ru Ho
    • Kuo-Wang Tsai
  • View Affiliations

  • Published online on: July 5, 2012     https://doi.org/10.3892/or.2012.1899
  • Pages: 1069-1076
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Abstract

MicroRNAs are small non-coding RNA molecules that play important roles in the multistep process of colorectal carcinoma (CRC) development. The present study evaluated the relationship between miR-1-1 and miR-133a-2 expression and DNA methylation, and its putative biological role in CRC. The results indicated that DNA methylation regulated the expression of the miR-1-1 and miR-133a-2 cluster in CRC cell lines. Expression of miR-1 and miR-133a was further evaluated in 64 paired tissue samples (CRC tumor and adjacent normal mucosa) using the stem-loop real-time polymerase chain reaction. The miR-1-133a cluster displayed significantly lower expression in CRC tissue compared to adjacent normal mucosa (P<0.001). The results also indicated frequent hypermethylation of the CpG islands upstream of miR-1-133a (54.6%). Liver metastatic tissues exhibited significantly lower miR-1 (P<0.001) and miR-133a (P<0.001) expression compared to adjacent normal mucosa. Expression of the miR-1-133a cluster inversely correlated with TAGLN2 in the tumor specimens. In conclusion, epigenetic repression of the miR-1-133a cluster may play a critical role in colorectal cancer metastasis by silencing TAGLN2.
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September 2012
Volume 28 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen W, Leung C, Pan H, Hu L, Li S, Ho M and Tsai K: Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer. Oncol Rep 28: 1069-1076, 2012
APA
Chen, W., Leung, C., Pan, H., Hu, L., Li, S., Ho, M., & Tsai, K. (2012). Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer. Oncology Reports, 28, 1069-1076. https://doi.org/10.3892/or.2012.1899
MLA
Chen, W., Leung, C., Pan, H., Hu, L., Li, S., Ho, M., Tsai, K."Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer". Oncology Reports 28.3 (2012): 1069-1076.
Chicago
Chen, W., Leung, C., Pan, H., Hu, L., Li, S., Ho, M., Tsai, K."Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer". Oncology Reports 28, no. 3 (2012): 1069-1076. https://doi.org/10.3892/or.2012.1899