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Article

Action of db-cAMP on the bystander effect and chemosensitivity through connexin 43 and Bcl-2-mediated pathways in medulloblastoma cells

  • Authors:
    • Peixin Sun
    • Yunhui Liu
    • Haoqiang Ying
    • Shaoyi Li
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang 110004, P.R. China
  • Pages: 969-976
    |
    Published online on: July 5, 2012
       https://doi.org/10.3892/or.2012.1900
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Abstract

Medulloblastoma (MB) is one of the most common malignant brain tumors of childhood and is associated with a poor prognosis. Gap-junctional intercellular communication (GJIC) is an important mode for cell-to-cell communication. Dysfunctional GJIC is exhibited in most cancer cells. There is significant evidence that GJIC is important in at least some prodrug/suicide gene systems by augmenting the bystander effect (BE). GJIC is made up of connexins (Cxs), among which Cx43 is present in most tissues. Bcl-2, an important apoptosis blocker, is closely associated with the sensitivity to anticancer drugs. Our study showed that dibutyryl cyclic adenosine monophosphate (db-cAMP) upregulated the Cx43 expression and GJIC function in Daoy medulloblastoma cells. It directly enhanced the BE using a herpes simplex virus thymidine kinase (HSV‑tk)/ganciclovir (GCV) system, which was blocked by a Cx43 inhibitor. In addition, db-cAMP increased the cytotoxicity of temozolomide and teniposide, possibly by downregulating the Bcl-2 expression and inducing apoptosis. Taken together, we demonstrated the beneficial effect of db-cAMP in treating medulloblastoma depending on the upregulation of BE and chemosensitivity through Cx43 and Bcl-2-mediated pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Sun P, Liu Y, Ying H and Li S: Action of db-cAMP on the bystander effect and chemosensitivity through connexin 43 and Bcl-2-mediated pathways in medulloblastoma cells. Oncol Rep 28: 969-976, 2012.
APA
Sun, P., Liu, Y., Ying, H., & Li, S. (2012). Action of db-cAMP on the bystander effect and chemosensitivity through connexin 43 and Bcl-2-mediated pathways in medulloblastoma cells. Oncology Reports, 28, 969-976. https://doi.org/10.3892/or.2012.1900
MLA
Sun, P., Liu, Y., Ying, H., Li, S."Action of db-cAMP on the bystander effect and chemosensitivity through connexin 43 and Bcl-2-mediated pathways in medulloblastoma cells". Oncology Reports 28.3 (2012): 969-976.
Chicago
Sun, P., Liu, Y., Ying, H., Li, S."Action of db-cAMP on the bystander effect and chemosensitivity through connexin 43 and Bcl-2-mediated pathways in medulloblastoma cells". Oncology Reports 28, no. 3 (2012): 969-976. https://doi.org/10.3892/or.2012.1900
Copy and paste a formatted citation
x
Spandidos Publications style
Sun P, Liu Y, Ying H and Li S: Action of db-cAMP on the bystander effect and chemosensitivity through connexin 43 and Bcl-2-mediated pathways in medulloblastoma cells. Oncol Rep 28: 969-976, 2012.
APA
Sun, P., Liu, Y., Ying, H., & Li, S. (2012). Action of db-cAMP on the bystander effect and chemosensitivity through connexin 43 and Bcl-2-mediated pathways in medulloblastoma cells. Oncology Reports, 28, 969-976. https://doi.org/10.3892/or.2012.1900
MLA
Sun, P., Liu, Y., Ying, H., Li, S."Action of db-cAMP on the bystander effect and chemosensitivity through connexin 43 and Bcl-2-mediated pathways in medulloblastoma cells". Oncology Reports 28.3 (2012): 969-976.
Chicago
Sun, P., Liu, Y., Ying, H., Li, S."Action of db-cAMP on the bystander effect and chemosensitivity through connexin 43 and Bcl-2-mediated pathways in medulloblastoma cells". Oncology Reports 28, no. 3 (2012): 969-976. https://doi.org/10.3892/or.2012.1900
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