Immortalized mouse fetal liver stromal cells support growth and maintenance of human embryonic stem cells

Hu,Jiabo; Hu,Sanqiang; Ma,Quanhui; Wang,Xiaohui; Zhou,Zhongwei; Zhang,Wei; Sun,Xiaochun; Zhu,Wei; Qian,Hui; Xu,Wenrong

doi:10.3892/or.2012.1909

Immortalized mouse fetal liver stromal cells support growth and maintenance of human embryonic stem cells

Authors:
- Jiabo Hu
- Sanqiang Hu
- Quanhui Ma
- Xiaohui Wang
- Zhongwei Zhou
- Wei Zhang
- Xiaochun Sun
- Wei Zhu
- Hui Qian
- Wenrong Xu
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Affiliations: School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, Department of Clinical Laboratory, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, Jiangsu 210028, P.R. China
Published online on: July 13, 2012 https://doi.org/10.3892/or.2012.1909
Pages: 1385-1391

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Abstract

Human embryonic stem cells (hESCs) are usually maintained in an undifferentiated state by co-culture with feeder cells. The feeder cells are important for the growth of hESCs. A novel spontaneously immortalizated mouse fetal liver stromal cell line, named KM3, was isolated from a 13.5‑day mouse fetal liver. In this study, we examined whether KM3 cells could be used as feeders to support the growth of hESCs. hESCs cultured on KM3 cells showed a similar proliferation rate and characteristics to mouse embryonic fibroblasts (MEFs) after prolonged culture, including morphology, unlimited and undifferentiated proliferative ability, maintenance of normal karyotypes, formation of embryoid bodies in vitro and typically immature teratomas in vivo. Our results indicate that the immortalized KM3 cell line has the potential to support the growth and maintenance of hESCs. The cell line may be used for the large-scale expansion of hESCs in a low-cost and less labor-intensive manner.

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