Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model

  • Authors:
    • Kanako Wakabayashi-Nakao
    • Koji Maruyama
    • Hidee Ishii
    • Koji Muramatsu
    • Keiichi Hatakeyama
    • Keiichi Ohshima
    • Shun-Ichiro Ogura
    • Takashi Nakajima
    • Ken Yamaguchi
    • Tohru Mochizuki
  • View Affiliations

  • Published online on: July 23, 2012     https://doi.org/10.3892/or.2012.1926
  • Pages: 1181-1186
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Abstract

Proneurotensin/neuromedin N (proNT/NMN), the precursor of neurotensin (NT) and neuromedin N (NMN), is produced by cancer tissues derived from the pancreas and colon. NT stimulates tumor growth and proliferation through its receptors; however, little is known about the precursor molecule in cancer tissues. We previously demonstrated that proNT/NMN is secreted from small cell lung carcinoma (SCLC) cell lines in serum-free conditioned medium, but not from non-small cell lung carcinoma (NSCLC) cell lines. It was suggested that this precursor may serve as a tumor marker for SCLC. In this study, we established in vivo xenograft models to evaluate the possibility of proNT/NMN as a specific tumor marker. SBC3 cells, derived from human SCLC, were inoculated into mice, and the proNT/NMN levels in plasma and tumor tissues were detected using specific antibodies. In contrast to control mouse plasma, the proNT/NMN levels in tumor-bearing mice increased as the tumors grew, and the elevated plasma proNT/NMN levels were decreased by tumor resection. Moreover, proNT/NMN was expressed in SBC3 tumors, suggesting that proNT/NMN was secreted into blood from the tumor, and this secretion may be specific to SCLC.
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October 2012
Volume 28 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wakabayashi-Nakao K, Maruyama K, Ishii H, Muramatsu K, Hatakeyama K, Ohshima K, Ogura S, Nakajima T, Yamaguchi K, Mochizuki T, Mochizuki T, et al: Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model. Oncol Rep 28: 1181-1186, 2012
APA
Wakabayashi-Nakao, K., Maruyama, K., Ishii, H., Muramatsu, K., Hatakeyama, K., Ohshima, K. ... Mochizuki, T. (2012). Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model. Oncology Reports, 28, 1181-1186. https://doi.org/10.3892/or.2012.1926
MLA
Wakabayashi-Nakao, K., Maruyama, K., Ishii, H., Muramatsu, K., Hatakeyama, K., Ohshima, K., Ogura, S., Nakajima, T., Yamaguchi, K., Mochizuki, T."Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model". Oncology Reports 28.4 (2012): 1181-1186.
Chicago
Wakabayashi-Nakao, K., Maruyama, K., Ishii, H., Muramatsu, K., Hatakeyama, K., Ohshima, K., Ogura, S., Nakajima, T., Yamaguchi, K., Mochizuki, T."Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model". Oncology Reports 28, no. 4 (2012): 1181-1186. https://doi.org/10.3892/or.2012.1926