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Article

Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model

  • Authors:
    • Kanako Wakabayashi-Nakao
    • Koji Maruyama
    • Hidee Ishii
    • Koji Muramatsu
    • Keiichi Hatakeyama
    • Keiichi Ohshima
    • Shun-Ichiro Ogura
    • Takashi Nakajima
    • Ken Yamaguchi
    • Tohru Mochizuki
  • View Affiliations / Copyright

    Affiliations: Medical Genetics Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan, Experimental Animal Facility, Shizuoka Cancer Center Research Institute, Shizuoka, Japan, Pathology Division, Shizuoka Cancer Center Hospital, Shizuoka, Japan, Frontier Research Center, Tokyo Institute of Technology, Kanagawa, Japan, Shizuoka Cancer Center Hospital and Research Institute, Shizuoka, Japan
  • Pages: 1181-1186
    |
    Published online on: July 23, 2012
       https://doi.org/10.3892/or.2012.1926
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Abstract

Proneurotensin/neuromedin N (proNT/NMN), the precursor of neurotensin (NT) and neuromedin N (NMN), is produced by cancer tissues derived from the pancreas and colon. NT stimulates tumor growth and proliferation through its receptors; however, little is known about the precursor molecule in cancer tissues. We previously demonstrated that proNT/NMN is secreted from small cell lung carcinoma (SCLC) cell lines in serum-free conditioned medium, but not from non-small cell lung carcinoma (NSCLC) cell lines. It was suggested that this precursor may serve as a tumor marker for SCLC. In this study, we established in vivo xenograft models to evaluate the possibility of proNT/NMN as a specific tumor marker. SBC3 cells, derived from human SCLC, were inoculated into mice, and the proNT/NMN levels in plasma and tumor tissues were detected using specific antibodies. In contrast to control mouse plasma, the proNT/NMN levels in tumor-bearing mice increased as the tumors grew, and the elevated plasma proNT/NMN levels were decreased by tumor resection. Moreover, proNT/NMN was expressed in SBC3 tumors, suggesting that proNT/NMN was secreted into blood from the tumor, and this secretion may be specific to SCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Wakabayashi-Nakao K, Maruyama K, Ishii H, Muramatsu K, Hatakeyama K, Ohshima K, Ogura S, Nakajima T, Yamaguchi K, Mochizuki T, Mochizuki T, et al: Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model. Oncol Rep 28: 1181-1186, 2012.
APA
Wakabayashi-Nakao, K., Maruyama, K., Ishii, H., Muramatsu, K., Hatakeyama, K., Ohshima, K. ... Mochizuki, T. (2012). Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model. Oncology Reports, 28, 1181-1186. https://doi.org/10.3892/or.2012.1926
MLA
Wakabayashi-Nakao, K., Maruyama, K., Ishii, H., Muramatsu, K., Hatakeyama, K., Ohshima, K., Ogura, S., Nakajima, T., Yamaguchi, K., Mochizuki, T."Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model". Oncology Reports 28.4 (2012): 1181-1186.
Chicago
Wakabayashi-Nakao, K., Maruyama, K., Ishii, H., Muramatsu, K., Hatakeyama, K., Ohshima, K., Ogura, S., Nakajima, T., Yamaguchi, K., Mochizuki, T."Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model". Oncology Reports 28, no. 4 (2012): 1181-1186. https://doi.org/10.3892/or.2012.1926
Copy and paste a formatted citation
x
Spandidos Publications style
Wakabayashi-Nakao K, Maruyama K, Ishii H, Muramatsu K, Hatakeyama K, Ohshima K, Ogura S, Nakajima T, Yamaguchi K, Mochizuki T, Mochizuki T, et al: Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model. Oncol Rep 28: 1181-1186, 2012.
APA
Wakabayashi-Nakao, K., Maruyama, K., Ishii, H., Muramatsu, K., Hatakeyama, K., Ohshima, K. ... Mochizuki, T. (2012). Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model. Oncology Reports, 28, 1181-1186. https://doi.org/10.3892/or.2012.1926
MLA
Wakabayashi-Nakao, K., Maruyama, K., Ishii, H., Muramatsu, K., Hatakeyama, K., Ohshima, K., Ogura, S., Nakajima, T., Yamaguchi, K., Mochizuki, T."Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model". Oncology Reports 28.4 (2012): 1181-1186.
Chicago
Wakabayashi-Nakao, K., Maruyama, K., Ishii, H., Muramatsu, K., Hatakeyama, K., Ohshima, K., Ogura, S., Nakajima, T., Yamaguchi, K., Mochizuki, T."Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model". Oncology Reports 28, no. 4 (2012): 1181-1186. https://doi.org/10.3892/or.2012.1926
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