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Article

Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells

  • Authors:
    • Li-Cai He
    • Feng-Hou Gao
    • Han-Zhang Xu
    • Shan Zhao
    • Chun-Min Ma
    • June Li
    • Shu Zhang
    • Ying-Li Wu
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education, Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Number 3 People's Hospital, Affiliated to Shanghai Jiao-Tong University School of Medicine (SJTU-SM), Shanghai 201900, P.R. China, Department of Genetics, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA, Department of Obstetrics and Gynecology, Renji Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine (SJTU-SM), Shanghai 200125, P.R. China
  • Pages: 1399-1405
    |
    Published online on: August 2, 2012
       https://doi.org/10.3892/or.2012.1946
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Abstract

The transcription factor Ikaros was originally found to function as a key regulator of lymphocyte differentiation. In this study, we provide the first evidence that Ikaros is expressed at higher levels in ovarian cancer tissues compared with normal ovarian tissues and is significantly associated with high FIGO stage and low differentiation state in ovarian serous adenocarcinoma. To this end, we transfected IK1 (full length of Ikaros) into the SKOV3 ovarian cancer cell line and examined cell biological behaviors including proliferation, migration and invasion. We found that overexpression of IK1 inhibited cell proliferation by inducing G1 arrest, accompanied by the upregulation of P27 and P21 and downregulation of cyclin D1 and D2. On the other hand, IK1 increased the migration and invasion of ovarian cancer cells, as assessed by scratch-wound assay, transwell migration assay, and invasion assay. Overexpression of IK1 significantly increased Slug but not Snail1 expression at both mRNA and protein levels. It also downregulated and upregulated E-cadherin and MMP-2, two target genes of Slug involved in migration, respectively. Furthermore, knocking down Slug abrogated IK1-mediated increase in migration and invasion. These data suggest that Slug plays an important role in IK1-induced migration and invasion. In conclusion, we show for the first time that IK1 plays a dual role in the proliferation, migration and invasion of ovarian cancer cells, providing new insights into their metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
He L, Gao F, Xu H, Zhao S, Ma C, Li J, Zhang S and Wu Y: Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells. Oncol Rep 28: 1399-1405, 2012.
APA
He, L., Gao, F., Xu, H., Zhao, S., Ma, C., Li, J. ... Wu, Y. (2012). Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells. Oncology Reports, 28, 1399-1405. https://doi.org/10.3892/or.2012.1946
MLA
He, L., Gao, F., Xu, H., Zhao, S., Ma, C., Li, J., Zhang, S., Wu, Y."Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells". Oncology Reports 28.4 (2012): 1399-1405.
Chicago
He, L., Gao, F., Xu, H., Zhao, S., Ma, C., Li, J., Zhang, S., Wu, Y."Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells". Oncology Reports 28, no. 4 (2012): 1399-1405. https://doi.org/10.3892/or.2012.1946
Copy and paste a formatted citation
x
Spandidos Publications style
He L, Gao F, Xu H, Zhao S, Ma C, Li J, Zhang S and Wu Y: Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells. Oncol Rep 28: 1399-1405, 2012.
APA
He, L., Gao, F., Xu, H., Zhao, S., Ma, C., Li, J. ... Wu, Y. (2012). Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells. Oncology Reports, 28, 1399-1405. https://doi.org/10.3892/or.2012.1946
MLA
He, L., Gao, F., Xu, H., Zhao, S., Ma, C., Li, J., Zhang, S., Wu, Y."Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells". Oncology Reports 28.4 (2012): 1399-1405.
Chicago
He, L., Gao, F., Xu, H., Zhao, S., Ma, C., Li, J., Zhang, S., Wu, Y."Ikaros inhibits proliferation and, through upregulation of Slug, increases metastatic ability of ovarian serous adenocarcinoma cells". Oncology Reports 28, no. 4 (2012): 1399-1405. https://doi.org/10.3892/or.2012.1946
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