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Article

Decreased expression of the carboxyl terminus of heat shock cognate 70 interacting protein in human gastric cancer and its clinical significance

  • Authors:
    • Lei Gan
    • Dong-Bo Liu
    • Hai-Feng Lu
    • Guo-Xian Long
    • Qi Mei
    • Guang-Yuan Hu
    • Hong Qiu
    • Guo-Qing Hu
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China, Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China
  • Pages: 1392-1398
    |
    Published online on: August 8, 2012
       https://doi.org/10.3892/or.2012.1957
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Abstract

The carboxyl terminus of heat shock cognate 70 interacting protein (CHIP) is an E3 ubiquitin ligase, which can promote ubiquitylation and degradation of many tumor-related proteins. However, the expression of CHIP in human gastric cancer has not been investigated. In this study, the mRNA and protein levels of CHIP expression in 53 cases of gastric cancer and matched normal tissues were determined by quantitative real-time PCR, western blotting and immunohistochemistry. We showed that CHIP was registered from basal to middle portions of normal gastric mucosa. CHIP expression was notably decreased or lost in human gastric cancer samples compared with the matched normal non-cancer samples. The correlations between CHIP downregulation and the clinicopathological characteristics were also evaluated. The expression of CHIP was significantly lower in the gastric cancer samples compared to the matched normal samples at both mRNA and protein levels (P<0.05 and P<0.05, respectively). More importantly, the downregulation of CHIP was correlated with TNM stage (P=0.048) and lymph node metastasis (P=0.010) at the mRNA levels. In addition, the downregulation of CHIP was correlated with lymph node metastasis (P=0.021) and tumor differentiation (P=0.009) at the protein levels. Taken together, at both mRNA and protein levels, the decreased expression of CHIP was correlated with lymph node metastasis. Furthermore, our study suggests that a negative correlation exists between CHIP expression and tumor malignancy in human gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Gan L, Liu D, Lu H, Long G, Mei Q, Hu G, Qiu H and Hu G: Decreased expression of the carboxyl terminus of heat shock cognate 70 interacting protein in human gastric cancer and its clinical significance. Oncol Rep 28: 1392-1398, 2012.
APA
Gan, L., Liu, D., Lu, H., Long, G., Mei, Q., Hu, G. ... Hu, G. (2012). Decreased expression of the carboxyl terminus of heat shock cognate 70 interacting protein in human gastric cancer and its clinical significance. Oncology Reports, 28, 1392-1398. https://doi.org/10.3892/or.2012.1957
MLA
Gan, L., Liu, D., Lu, H., Long, G., Mei, Q., Hu, G., Qiu, H., Hu, G."Decreased expression of the carboxyl terminus of heat shock cognate 70 interacting protein in human gastric cancer and its clinical significance". Oncology Reports 28.4 (2012): 1392-1398.
Chicago
Gan, L., Liu, D., Lu, H., Long, G., Mei, Q., Hu, G., Qiu, H., Hu, G."Decreased expression of the carboxyl terminus of heat shock cognate 70 interacting protein in human gastric cancer and its clinical significance". Oncology Reports 28, no. 4 (2012): 1392-1398. https://doi.org/10.3892/or.2012.1957
Copy and paste a formatted citation
x
Spandidos Publications style
Gan L, Liu D, Lu H, Long G, Mei Q, Hu G, Qiu H and Hu G: Decreased expression of the carboxyl terminus of heat shock cognate 70 interacting protein in human gastric cancer and its clinical significance. Oncol Rep 28: 1392-1398, 2012.
APA
Gan, L., Liu, D., Lu, H., Long, G., Mei, Q., Hu, G. ... Hu, G. (2012). Decreased expression of the carboxyl terminus of heat shock cognate 70 interacting protein in human gastric cancer and its clinical significance. Oncology Reports, 28, 1392-1398. https://doi.org/10.3892/or.2012.1957
MLA
Gan, L., Liu, D., Lu, H., Long, G., Mei, Q., Hu, G., Qiu, H., Hu, G."Decreased expression of the carboxyl terminus of heat shock cognate 70 interacting protein in human gastric cancer and its clinical significance". Oncology Reports 28.4 (2012): 1392-1398.
Chicago
Gan, L., Liu, D., Lu, H., Long, G., Mei, Q., Hu, G., Qiu, H., Hu, G."Decreased expression of the carboxyl terminus of heat shock cognate 70 interacting protein in human gastric cancer and its clinical significance". Oncology Reports 28, no. 4 (2012): 1392-1398. https://doi.org/10.3892/or.2012.1957
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