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November 2012 Volume 28 Issue 5

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Article

Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer

  • Authors:
    • Danjie Zhang
    • Wei Zhao
    • Xinhua Liao
    • Tieqiang Bi
    • Haijun Li
    • Xiangming Che
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an JiaoTong University, Xi'an 710061, P.R. China
  • Pages: 1785-1791
    |
    Published online on: August 30, 2012
       https://doi.org/10.3892/or.2012.1997
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Abstract

The cadherins are a family of cell surface glycoproteins responsible for cell adhesion which play an important role in cell morphology, contact inhibition and signal transduction during tumorigenesis. Protocadherin 8 (PCDH8), a member of the cadherin family, has been reported to act as a tumor suppressor involved in oncogenesis in breast cancer. In this study, we aimed to investigate the epigenetic inactivation of PCDH8 and its tumor suppressor function in gastric cancer. The expression of PCDH8 was markedly reduced or silenced in gastric cancer cell lines compared with normal gastric cells or tissues. Methylation of the PCDH8 gene promoter was observed in 100% (4/4) of cell lines and 55.38% (36/65) of the primary gastric cancer by methylation-specific PCR, but not in normal gastric mucosa (0/10). Methylated PCDH8 was significantly associated with lymph node metastasis in a logistic regression analysis. The demethylation reagent 5-aza-2'-deoxycytidine was able to restore or upregulate PCDH8 expression in gastric cancer cell lines. Ectopic expression of PCDH8 in silenced gastric cancer cells significantly inhibited cell migration and induced apoptosis. For the first time, our study demonstrates the epigenetic inactivation of PCDH8 by promoter methylation and its tumor suppressor function in human gastric cancer. Thus, PCDH8 could be identified as a candidate tumor suppressor in human gastric cancer.
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1 

Herszényi L and Tulassay Z: Epidemiology of gastrointestinal and liver tumors. Eur Rev Med Pharmacol Sci. 14:249–258. 2010.

2 

Brenner H, Rothenbacher D and Arndt V: Epidemiology of stomach cancer. Methods Mol Biol. 472:467–477. 2009. View Article : Google Scholar

3 

Arkenau HT: Gastric cancer in the era of molecularly targeted agents: current drug development strategies. J Cancer Res Clin Oncol. 135:855–866. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Yasui W, Sentani K, Motoshita J, et al: Molecular pathobiology of gastric cancer. Scand J Surg. 95:225–231. 2006.

5 

Herman JG and Baylin SB: Gene silencing in cancer in association with promoter hypermethylation. N Engl J Med. 349:2042–2054. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Wang JF and Dai DQ: Metastatic suppressor genes inactivated by aberrant methylation in gastric cancer. World J Gastroenterol. 13:5692–5698. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Machado JC, Oliveira C, Carvalho R, et al: E-cadherin gene (CDH1) promoter methylation as the second hit in sporadic diffuse gastric carcinoma. Oncology. 20:1525–1528. 2001.PubMed/NCBI

8 

Esteller M, Corn PG, Baylin SB, et al: A gene hypermethylation profile of human cancer. Cancer Res. 61:3225–3229. 2001.PubMed/NCBI

9 

Buffart TE, Overmeer RM, Steenbergen RD, et al: MAL promoter hypermethylation as a novel prognostic marker in gastric cancer. Br J Cancer. 99:1802–1807. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Nishikawa Y, Miyazaki T, Nakashiro K, et al: Human FAT1 cadherin controls cell migration and invasion of oral squamous cell carcinoma through the localization of β-catenin. Oncol Rep. 26:587–592. 2011.PubMed/NCBI

11 

Gumbiner BM: Regulation of cadherin-mediated adhesion in morphogenesis. Nat Rev Mol Cell Biol. 6:622–634. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Batlle E, Sancho E, Franci C, et al: The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells. Nat Cell Biol. 2:84–89. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Imoto I, Izumi H, Yokoi S, Hosoda H, et al: Frequent silencing of the candidate tumor suppressor PCDH20 by epigenetic mechanism in non-small-cell lung cancers. Cancer Res. 66:4617–4626. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Ying J, Li H, Seng TJ, Langford C, et al: Functional epigenetics identifies a protocadherin PCDH10 as a candidate tumor suppressor for nasopharyngeal, esophagealand multiple other carcinomas with frequent methylation. Oncogene. 25:1070–1080. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Haruki S, Imoto I, Kozaki K, et al: Frequent silencing of protocadherin 17, a candidate tumour suppressor for esophageal squamous-cell carcinoma. Carcinogenesis. 31:1027–1036. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Strehl S, Glatt K, Liu QM, et al: Characterization of two novel protocadherins (PCDH8 and PCDH9) localized on human chromosome 13 and mouse chromosome 14. Gemomics. 53:81–89. 1998.PubMed/NCBI

17 

Yu JS, Koujak S, Nagase S, et al: PCDH8, the human homolog of PAPC, is a candidate tumor suppressor of breast cancer. Oncogene. 27:4657–4665. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Herman JG, Graff JR, Myöhänen S, et al: Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA. 93:9821–9826. 1996. View Article : Google Scholar : PubMed/NCBI

19 

Gorringe KL, Ramakrishna M, Williams LH, et al: Are there any more ovarian tumor suppressor genes? A new perspective using ultra high-resolution copy number and loss of heterozygosity analysis. Genes Chromosomes Cancer. 48:931–942. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Franko J, Krasinskas AM, Nikiforova MN, et al: Loss of heterozygosity predicts poor survival after resection of pancreatic adenocarcinoma. J Gastrointest Surg. 12:1664–1672. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Chang YC, Yeh KT, Liu TC, et al: Molecular cytogenetic characterization of esophageal cancer detected by comparative genomic hybridization. J Clin Lab Anal. 24:167–174. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Ye Y, McDevitt MA, Guo M, et al: Progressive chromatin repression and promoter methylation of CTNNA1 associated with advanced myeloid malignancies. Cancer Res. 69:8482–8490. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Hukriede NA, Tsang TE, Habas R, et al: Conserved requirement of Lim1 function for cell movements during gastrulation. Dev Cell. 4:83–94. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Medina A, Swain RK, Kuerner KM and Steinbeisser H: Xenopus paraxial protocadherin has signaling functions and is involved in tissue separation. EMBO J. 23:3249–3258. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Unterseher F, Hefele JA, Giehl K, et al: Paraxial protocadherin coordinates cell polarity during convergent extension via Rho A and JNK. EMBO J. 23:3259–3269. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Tian K, Jurukovski V, Wang XP, et al: Epigenetic regulation of WTH3 in primary and cultured drugresistant breast cancer cells. Cancer Res. 65:10024–10031. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang D, Zhao W, Liao X, Bi T, Li H and Che X: Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer. Oncol Rep 28: 1785-1791, 2012.
APA
Zhang, D., Zhao, W., Liao, X., Bi, T., Li, H., & Che, X. (2012). Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer. Oncology Reports, 28, 1785-1791. https://doi.org/10.3892/or.2012.1997
MLA
Zhang, D., Zhao, W., Liao, X., Bi, T., Li, H., Che, X."Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer". Oncology Reports 28.5 (2012): 1785-1791.
Chicago
Zhang, D., Zhao, W., Liao, X., Bi, T., Li, H., Che, X."Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer". Oncology Reports 28, no. 5 (2012): 1785-1791. https://doi.org/10.3892/or.2012.1997
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang D, Zhao W, Liao X, Bi T, Li H and Che X: Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer. Oncol Rep 28: 1785-1791, 2012.
APA
Zhang, D., Zhao, W., Liao, X., Bi, T., Li, H., & Che, X. (2012). Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer. Oncology Reports, 28, 1785-1791. https://doi.org/10.3892/or.2012.1997
MLA
Zhang, D., Zhao, W., Liao, X., Bi, T., Li, H., Che, X."Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer". Oncology Reports 28.5 (2012): 1785-1791.
Chicago
Zhang, D., Zhao, W., Liao, X., Bi, T., Li, H., Che, X."Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer". Oncology Reports 28, no. 5 (2012): 1785-1791. https://doi.org/10.3892/or.2012.1997
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