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Article

Protease-activated receptor-2 induces expression of vascular endothelial growth factor and cyclooxygenase-2 via the mitogen-activated protein kinase pathway in gastric cancer cells

  • Authors:
    • Cheng Zhang
    • Guang-Rong Gao
    • Chen-Guang Lv
    • Boa-Lei Zhang
    • Zhi-Li Zhang
    • Xue-Feng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110840, P.R. China
  • Pages: 1917-1923
    |
    Published online on: August 30, 2012
       https://doi.org/10.3892/or.2012.1998
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Abstract

Protease-activated receptor-2 (PAR-2) has shown strong pro-angiogenesis activity physiologically and pathologically. This study aimed to explore PAR-2 regulation of pro-angiogenesis gene expression and the underlying molecular pathways in gastric cancer cells. MKN28 human gastric cancer cells were treated with trypsin, a PAR-2 activator, and subjected to real-time reverse transcription polymerase chain reaction (qRT-PCR), western blotting and ELISA for gene expression analyses. ERK1/2 phosphorylation and p38 MAP kinase inhibitors (PD98059 and SB203580, respectively) were used to block their gene activities. PAR-2 mRNA and protein were expressed in MKN-28 cells and activated by trypsin treatment. Trypsin-activated PAR-2 protein significantly enhanced expression of vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) mRNA and protein in gastric cancer cells in a dose- and time-dependent manner. PAR-2 activation also induced the phosphorylation of ERK1/2 and p38 MAP kinase, but the ERK1/2 and p38 inhibitors blocked the activated PAR-2-induced VEGF and COX-2 expression in gastric cancer cells. PAR-2-induced expression of VEGF and COX-2 mRNA and protein in gastric cancer MKN28 cells was mediated by activation of an ERK1/2- and p38 MAP kinase-dependent pathway. Thus, PAR-2 may serve as a promising target for anti-angiogenesis therapy to treat gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang C, Gao G, Lv C, Zhang B, Zhang Z and Zhang X: Protease-activated receptor-2 induces expression of vascular endothelial growth factor and cyclooxygenase-2 via the mitogen-activated protein kinase pathway in gastric cancer cells. Oncol Rep 28: 1917-1923, 2012.
APA
Zhang, C., Gao, G., Lv, C., Zhang, B., Zhang, Z., & Zhang, X. (2012). Protease-activated receptor-2 induces expression of vascular endothelial growth factor and cyclooxygenase-2 via the mitogen-activated protein kinase pathway in gastric cancer cells. Oncology Reports, 28, 1917-1923. https://doi.org/10.3892/or.2012.1998
MLA
Zhang, C., Gao, G., Lv, C., Zhang, B., Zhang, Z., Zhang, X."Protease-activated receptor-2 induces expression of vascular endothelial growth factor and cyclooxygenase-2 via the mitogen-activated protein kinase pathway in gastric cancer cells". Oncology Reports 28.5 (2012): 1917-1923.
Chicago
Zhang, C., Gao, G., Lv, C., Zhang, B., Zhang, Z., Zhang, X."Protease-activated receptor-2 induces expression of vascular endothelial growth factor and cyclooxygenase-2 via the mitogen-activated protein kinase pathway in gastric cancer cells". Oncology Reports 28, no. 5 (2012): 1917-1923. https://doi.org/10.3892/or.2012.1998
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C, Gao G, Lv C, Zhang B, Zhang Z and Zhang X: Protease-activated receptor-2 induces expression of vascular endothelial growth factor and cyclooxygenase-2 via the mitogen-activated protein kinase pathway in gastric cancer cells. Oncol Rep 28: 1917-1923, 2012.
APA
Zhang, C., Gao, G., Lv, C., Zhang, B., Zhang, Z., & Zhang, X. (2012). Protease-activated receptor-2 induces expression of vascular endothelial growth factor and cyclooxygenase-2 via the mitogen-activated protein kinase pathway in gastric cancer cells. Oncology Reports, 28, 1917-1923. https://doi.org/10.3892/or.2012.1998
MLA
Zhang, C., Gao, G., Lv, C., Zhang, B., Zhang, Z., Zhang, X."Protease-activated receptor-2 induces expression of vascular endothelial growth factor and cyclooxygenase-2 via the mitogen-activated protein kinase pathway in gastric cancer cells". Oncology Reports 28.5 (2012): 1917-1923.
Chicago
Zhang, C., Gao, G., Lv, C., Zhang, B., Zhang, Z., Zhang, X."Protease-activated receptor-2 induces expression of vascular endothelial growth factor and cyclooxygenase-2 via the mitogen-activated protein kinase pathway in gastric cancer cells". Oncology Reports 28, no. 5 (2012): 1917-1923. https://doi.org/10.3892/or.2012.1998
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