Identification of the cancer/testis antigens AKAP3 and CTp11 by SEREX in hepatocellular carcinoma

  • Authors:
    • Myung-Ha Song
    • Kyung-Un Choi
    • Dong-Hoon Shin
    • Chang-Hun Lee
    • Sang-Yull Lee
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  • Published online on: August 30, 2012     https://doi.org/10.3892/or.2012.2002
  • Pages: 1792-1798
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Abstract

Cancer/testis (CT) antigens are considered promising target molecules for immunotherapy. To efficiently identify potential CT antigens, a testis cDNA library was immunoscreened with sera from hepatocellular carcinoma (HCC) patients. We isolated 3 different antigens, AKAP3, CTp11, and UBQLN3. Although AKAP3 and CTp11 have been previously reported as CT antigens, this is the first time that these 2 antigens have been isolated from HCC patients by SEREX. Conventional RT-PCR analysis showed that AKAP3 was frequently present in HCC cell lines (5/7) and HCC tissues (5/10), and the gene was broadly expressed in several cancer types, including breast cancer cell lines (3/6), breast cancer tissues (6/9), colon cancer cell lines (3/10), colon cancer tissues (5/6), ovary cancer cell lines (6/8), ovary cancer tissues (11/16), lung cancer cell lines (4/7) and lung cancer tissues (6/13). By phage plaque analysis, anti-AKAP3 antibody was detected in sera from 15 of 27 HCC patients and 8 of 27 healthy donors. These data suggest that AKAP3 may be useful for diagnosis and immunotherapy in HCC patients.
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November 2012
Volume 28 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Song M, Choi K, Shin D, Lee C and Lee S: Identification of the cancer/testis antigens AKAP3 and CTp11 by SEREX in hepatocellular carcinoma. Oncol Rep 28: 1792-1798, 2012
APA
Song, M., Choi, K., Shin, D., Lee, C., & Lee, S. (2012). Identification of the cancer/testis antigens AKAP3 and CTp11 by SEREX in hepatocellular carcinoma. Oncology Reports, 28, 1792-1798. https://doi.org/10.3892/or.2012.2002
MLA
Song, M., Choi, K., Shin, D., Lee, C., Lee, S."Identification of the cancer/testis antigens AKAP3 and CTp11 by SEREX in hepatocellular carcinoma". Oncology Reports 28.5 (2012): 1792-1798.
Chicago
Song, M., Choi, K., Shin, D., Lee, C., Lee, S."Identification of the cancer/testis antigens AKAP3 and CTp11 by SEREX in hepatocellular carcinoma". Oncology Reports 28, no. 5 (2012): 1792-1798. https://doi.org/10.3892/or.2012.2002