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Article

Growth inhibition and apoptosis of human B-cell lymphoma in vitro and in vivo by Bcl-2 short hairpin RNA

  • Authors:
    • Yuchun Liu
    • Yongmei Shen
    • Chenhao Qin
    • Yizhen Shi
    • Guang Rong
    • Xilong Yu
  • View Affiliations / Copyright

    Affiliations: Radioimmunoassay Center, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Clinical Laboratory, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China
  • Pages: 244-252
    |
    Published online on: October 16, 2012
       https://doi.org/10.3892/or.2012.2081
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Abstract

Bcl-2 is overexpressed in various types of human tumors, including Burkitt's lymphoma, and it is involved in tumorigenesis and chemoresistance, therefore, it is regarded as a potential target of gene therapy. In this study, RNA interference using short hairpin RNA (shRNA)-mediated RNA interference was introduced into Burkitt's lymphoma Raji cells to validate its effects on Bcl-2 expression and cell proliferation in vitro and in vivo. We constructed two types of Bcl-2 shRNA plasmid (pGenesil-1-Bcl-2-1 and pGenesil-1-Bcl-2-2) and negative control shRNA plasmid (pGenesil-1-NC) and stably transfected them into Raji cells. The expression levels of Bcl-2 mRNA and protein were assayed by RT-PCR, flow cytometry and western blotting. Cell proliferation was determined by cell count assay. The antitumor activities and apoptosis of the two types of Bcl-2 shRNA plasmid were evaluated in BALB/c nude mice bearing Burkitt's lymphoma inoculated with Raji cells. The results showed that the expression levels of Bcl-2 mRNA and protein decreased, compared with either the pGenecil-1-NC or the untransfected cell group (P<0.05). The cell proliferation assay showed that Bcl-2 shRNA significantly inhibited the growth of Raji cells (P<0.01). Furthermore, the tumor growth of the Bcl-2 shRNA cell group was dramatically lower and smaller than that of the negative control or untransfected cell group (P<0.01). Bcl-2 protein expression in the untransfected and the pGenesil-1-NC group were markedly higher than that of the pGenesil-1-Bcl-2-1 and the pGenesil-1-Bcl-2 group by immunohistochemistry (both P<0.01) and the results using transmission electron microscopy showed that Bcl-2 shRNA significantly induced Raji cell apoptosis. Additionally, the inhibition effect of pGenesil-1-Bcl-2-1 was better than that of pGenesil-1-Bcl-2-2. It has been suggested that vector-based Bcl-2 shRNA could effectively reduce the expression of Bcl-2 and induce apoptosis and growth inhibition of Burkitt's lymphoma Raji cells. Vector-based Bcl-2 shRNA could be a potential gene therapeutic strategy against human Burkitt's lymphoma.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Shen Y, Qin C, Shi Y, Rong G and Yu X: Growth inhibition and apoptosis of human B-cell lymphoma in vitro and in vivo by Bcl-2 short hairpin RNA. Oncol Rep 29: 244-252, 2013.
APA
Liu, Y., Shen, Y., Qin, C., Shi, Y., Rong, G., & Yu, X. (2013). Growth inhibition and apoptosis of human B-cell lymphoma in vitro and in vivo by Bcl-2 short hairpin RNA. Oncology Reports, 29, 244-252. https://doi.org/10.3892/or.2012.2081
MLA
Liu, Y., Shen, Y., Qin, C., Shi, Y., Rong, G., Yu, X."Growth inhibition and apoptosis of human B-cell lymphoma in vitro and in vivo by Bcl-2 short hairpin RNA". Oncology Reports 29.1 (2013): 244-252.
Chicago
Liu, Y., Shen, Y., Qin, C., Shi, Y., Rong, G., Yu, X."Growth inhibition and apoptosis of human B-cell lymphoma in vitro and in vivo by Bcl-2 short hairpin RNA". Oncology Reports 29, no. 1 (2013): 244-252. https://doi.org/10.3892/or.2012.2081
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Shen Y, Qin C, Shi Y, Rong G and Yu X: Growth inhibition and apoptosis of human B-cell lymphoma in vitro and in vivo by Bcl-2 short hairpin RNA. Oncol Rep 29: 244-252, 2013.
APA
Liu, Y., Shen, Y., Qin, C., Shi, Y., Rong, G., & Yu, X. (2013). Growth inhibition and apoptosis of human B-cell lymphoma in vitro and in vivo by Bcl-2 short hairpin RNA. Oncology Reports, 29, 244-252. https://doi.org/10.3892/or.2012.2081
MLA
Liu, Y., Shen, Y., Qin, C., Shi, Y., Rong, G., Yu, X."Growth inhibition and apoptosis of human B-cell lymphoma in vitro and in vivo by Bcl-2 short hairpin RNA". Oncology Reports 29.1 (2013): 244-252.
Chicago
Liu, Y., Shen, Y., Qin, C., Shi, Y., Rong, G., Yu, X."Growth inhibition and apoptosis of human B-cell lymphoma in vitro and in vivo by Bcl-2 short hairpin RNA". Oncology Reports 29, no. 1 (2013): 244-252. https://doi.org/10.3892/or.2012.2081
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