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Article

MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells

  • Authors:
    • Yong Lu
    • Michael Chopp
    • Xuguang Zheng
    • Mark Katakowski
    • Benjamin Buller
    • Feng Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Henry Ford Hospital, Detroit, MI, USA
  • Pages: 67-72
    |
    Published online on: October 17, 2012
       https://doi.org/10.3892/or.2012.2084
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Abstract

MicroRNAs are important regulators of gene expression and have been suggested to play a key role in tumorigenesis. In this study, we show that miR-145 is significantly downregulated in glioma cell lines compared to normal brain tissue and negatively regulates tumorigenesis. Restoration of miR-145 in glioma cells significantly reduced in vitro proliferation, migration and invasion. Also, overexpression of miR-145 reduced ADAM17 and EGFR expression. In addition, we tested the hypothesis that the miR-145-mediated suppression of cell proliferation, migration and invasion is, at least in part, due to silencing of ADAM17 and EGFR gene expression. Using luciferase reporters carrying the 3'-untranslated region of ADAM17 combined with western blotting, we identified ADAM17 as a direct target of miR-145. Collectively, these results suggest that as a tumor suppressor, miR-145 inhibits not only tumor proliferation, but also cell migration and invasion, and warrants further investigation.
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Copy and paste a formatted citation
Spandidos Publications style
Lu Y, Chopp M, Zheng X, Katakowski M, Buller B and Jiang F: MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells. Oncol Rep 29: 67-72, 2013.
APA
Lu, Y., Chopp, M., Zheng, X., Katakowski, M., Buller, B., & Jiang, F. (2013). MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells. Oncology Reports, 29, 67-72. https://doi.org/10.3892/or.2012.2084
MLA
Lu, Y., Chopp, M., Zheng, X., Katakowski, M., Buller, B., Jiang, F."MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells". Oncology Reports 29.1 (2013): 67-72.
Chicago
Lu, Y., Chopp, M., Zheng, X., Katakowski, M., Buller, B., Jiang, F."MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells". Oncology Reports 29, no. 1 (2013): 67-72. https://doi.org/10.3892/or.2012.2084
Copy and paste a formatted citation
x
Spandidos Publications style
Lu Y, Chopp M, Zheng X, Katakowski M, Buller B and Jiang F: MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells. Oncol Rep 29: 67-72, 2013.
APA
Lu, Y., Chopp, M., Zheng, X., Katakowski, M., Buller, B., & Jiang, F. (2013). MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells. Oncology Reports, 29, 67-72. https://doi.org/10.3892/or.2012.2084
MLA
Lu, Y., Chopp, M., Zheng, X., Katakowski, M., Buller, B., Jiang, F."MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells". Oncology Reports 29.1 (2013): 67-72.
Chicago
Lu, Y., Chopp, M., Zheng, X., Katakowski, M., Buller, B., Jiang, F."MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells". Oncology Reports 29, no. 1 (2013): 67-72. https://doi.org/10.3892/or.2012.2084
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