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Article

Plasma and tissue proteomic prognostic factors of response in primary breast cancer patients receiving neoadjuvant chemotherapy

  • Authors:
    • J. Bonneterre
    • F. Révillion
    • C. Desauw
    • E. Blot
    • A. Kramar
    • C. Fournier
    • L. Hornez
    • J. P. Peyrat
  • View Affiliations / Copyright

    Affiliations: Oscar Lambret Center, F-59000 Lille, France, Regional University Hospital, F-59037 Lille Cedex, France, Henri Becquerel Center, F-76038 Rouen Cedex, France
  • Pages: 355-361
    |
    Published online on: October 19, 2012
       https://doi.org/10.3892/or.2012.2090
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Abstract

A pathological complete response (pCR) after neoadjuvant chemotherapy is observed in approximately 20% of breast cancer patients. A proteomic analysis was performed on plasma and tumor tissue before treatment to evaluate its potential impact on the prediction of response. One hundred and forty-nine breast cancer patients eligible for neoadjuvant chemotherapy were included in the study between February 2004 and January 2009 at three centers. The proteomic analysis was performed using SELDI Technology (ProteinChip CM10 pH4, IMAC-Cu and H50). Three acquisition protocols were used according to the mass range. Plasma and tumor proteomic signatures were generated using generalized ROC criteria and cross-validation. Twenty-eight (18.8%) patients out of 149 experienced a pCR according to Sataloff criteria. In the cytosol analysis, respectively 4, 2 and 8 proteins had significantly different levels of expression in the responders and non-responders using IMAC-Cu, H50 and CM10 pH4. Among the 8 proteins of interest on CM10 pH4, 2 (C1 and C7) were selected and were validated in 95.0 and 85.6% of the models. In the plasma analysis, respectively 12, 6 and 2 proteins had different levels of expression using the same proteinchips. Among the 12 plasma proteins of interest on IMAC-Cu, 2 (P1 and P7) were selected and were validated in 94.8 and 97.6% of the models. A combined proteomic signature was generated, which remained statistically significant when adjusted for hormone receptor status and Ki-67. Our results show that proteomic analysis can differentiate complete pathological responders in breast cancer patients after neoadjuvant chemotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Bonneterre J, Révillion F, Desauw C, Blot E, Kramar A, Fournier C, Hornez L and Peyrat JP: Plasma and tissue proteomic prognostic factors of response in primary breast cancer patients receiving neoadjuvant chemotherapy. Oncol Rep 29: 355-361, 2013.
APA
Bonneterre, J., Révillion, F., Desauw, C., Blot, E., Kramar, A., Fournier, C. ... Peyrat, J.P. (2013). Plasma and tissue proteomic prognostic factors of response in primary breast cancer patients receiving neoadjuvant chemotherapy. Oncology Reports, 29, 355-361. https://doi.org/10.3892/or.2012.2090
MLA
Bonneterre, J., Révillion, F., Desauw, C., Blot, E., Kramar, A., Fournier, C., Hornez, L., Peyrat, J. P."Plasma and tissue proteomic prognostic factors of response in primary breast cancer patients receiving neoadjuvant chemotherapy". Oncology Reports 29.1 (2013): 355-361.
Chicago
Bonneterre, J., Révillion, F., Desauw, C., Blot, E., Kramar, A., Fournier, C., Hornez, L., Peyrat, J. P."Plasma and tissue proteomic prognostic factors of response in primary breast cancer patients receiving neoadjuvant chemotherapy". Oncology Reports 29, no. 1 (2013): 355-361. https://doi.org/10.3892/or.2012.2090
Copy and paste a formatted citation
x
Spandidos Publications style
Bonneterre J, Révillion F, Desauw C, Blot E, Kramar A, Fournier C, Hornez L and Peyrat JP: Plasma and tissue proteomic prognostic factors of response in primary breast cancer patients receiving neoadjuvant chemotherapy. Oncol Rep 29: 355-361, 2013.
APA
Bonneterre, J., Révillion, F., Desauw, C., Blot, E., Kramar, A., Fournier, C. ... Peyrat, J.P. (2013). Plasma and tissue proteomic prognostic factors of response in primary breast cancer patients receiving neoadjuvant chemotherapy. Oncology Reports, 29, 355-361. https://doi.org/10.3892/or.2012.2090
MLA
Bonneterre, J., Révillion, F., Desauw, C., Blot, E., Kramar, A., Fournier, C., Hornez, L., Peyrat, J. P."Plasma and tissue proteomic prognostic factors of response in primary breast cancer patients receiving neoadjuvant chemotherapy". Oncology Reports 29.1 (2013): 355-361.
Chicago
Bonneterre, J., Révillion, F., Desauw, C., Blot, E., Kramar, A., Fournier, C., Hornez, L., Peyrat, J. P."Plasma and tissue proteomic prognostic factors of response in primary breast cancer patients receiving neoadjuvant chemotherapy". Oncology Reports 29, no. 1 (2013): 355-361. https://doi.org/10.3892/or.2012.2090
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