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Article

Norcantharidin suppresses cell growth and migration with enhanced anticancer activity of gefitinib and cisplatin in human non-small cell lung cancer cells

  • Authors:
    • Ya-Chun Lee
    • Liang-Ming Lee
    • Chih-Hsin Yang
    • Anya Maan-Yuh Lin
    • Yi-Chia Huang
    • Chia-Chi Hsu
    • Meng-Shian Chen
    • Chin-Wen Chi
    • Pen-Hui Yin
    • Cheng-Deng Kuo
    • Jyh-Fei Liao
    • Hsin-Chen Lee
  • View Affiliations / Copyright

    Affiliations: Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C., Department of Urology, Taipei Medical University-Affiliated Taipei Municipal Wan-Fang Hospital, Taipei, Taiwan, R.O.C., Department of Oncology, National Taiwan University Hospital, Taipei, Taiwan, R.O.C., Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.
  • Pages: 237-243
    |
    Published online on: October 31, 2012
       https://doi.org/10.3892/or.2012.2118
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Abstract

Norcantharidin is the demethylated analog of cantharidin isolated from blister beetles (Mylabris phalerata Pall.). In this study, we evaluated whether norcantharidin exhibits anticancer effects against the human non-small cell lung cancer cell lines A549 (epidermal growth factor receptor (EGFR) mutation-negative) and PC9 (EGFR mutation-positive). Our results revealed that norcantharidin dose-dependently retards cell growth, arrests cell cycle at G2/M phase, reduces cell migration, and even induces apoptosis at the concentration of 100 µM. Moreover, we found that norcantharidin enhances the anticancer effects of gefitinib and cisplatin. Norcantharidin exhibited similar potency of anticancer effects against the two cell lines with different EGFR mutation status and did not affect EGF-induced EGFR phosphorylation, suggesting that the EGFR signaling may not be the target of norcantharidin. In conclusion, our results suggest that norcantharidin exhibits anticancer effects against non-small cell lung cancer cells in vitro and support its potential as a chemotherapeutic agent for treating non-small cell lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Lee Y, Lee L, Yang C, Lin AM, Huang Y, Hsu C, Chen M, Chi C, Yin P, Kuo C, Kuo C, et al: Norcantharidin suppresses cell growth and migration with enhanced anticancer activity of gefitinib and cisplatin in human non-small cell lung cancer cells. Oncol Rep 29: 237-243, 2013.
APA
Lee, Y., Lee, L., Yang, C., Lin, A.M., Huang, Y., Hsu, C. ... Lee, H. (2013). Norcantharidin suppresses cell growth and migration with enhanced anticancer activity of gefitinib and cisplatin in human non-small cell lung cancer cells. Oncology Reports, 29, 237-243. https://doi.org/10.3892/or.2012.2118
MLA
Lee, Y., Lee, L., Yang, C., Lin, A. M., Huang, Y., Hsu, C., Chen, M., Chi, C., Yin, P., Kuo, C., Liao, J., Lee, H."Norcantharidin suppresses cell growth and migration with enhanced anticancer activity of gefitinib and cisplatin in human non-small cell lung cancer cells". Oncology Reports 29.1 (2013): 237-243.
Chicago
Lee, Y., Lee, L., Yang, C., Lin, A. M., Huang, Y., Hsu, C., Chen, M., Chi, C., Yin, P., Kuo, C., Liao, J., Lee, H."Norcantharidin suppresses cell growth and migration with enhanced anticancer activity of gefitinib and cisplatin in human non-small cell lung cancer cells". Oncology Reports 29, no. 1 (2013): 237-243. https://doi.org/10.3892/or.2012.2118
Copy and paste a formatted citation
x
Spandidos Publications style
Lee Y, Lee L, Yang C, Lin AM, Huang Y, Hsu C, Chen M, Chi C, Yin P, Kuo C, Kuo C, et al: Norcantharidin suppresses cell growth and migration with enhanced anticancer activity of gefitinib and cisplatin in human non-small cell lung cancer cells. Oncol Rep 29: 237-243, 2013.
APA
Lee, Y., Lee, L., Yang, C., Lin, A.M., Huang, Y., Hsu, C. ... Lee, H. (2013). Norcantharidin suppresses cell growth and migration with enhanced anticancer activity of gefitinib and cisplatin in human non-small cell lung cancer cells. Oncology Reports, 29, 237-243. https://doi.org/10.3892/or.2012.2118
MLA
Lee, Y., Lee, L., Yang, C., Lin, A. M., Huang, Y., Hsu, C., Chen, M., Chi, C., Yin, P., Kuo, C., Liao, J., Lee, H."Norcantharidin suppresses cell growth and migration with enhanced anticancer activity of gefitinib and cisplatin in human non-small cell lung cancer cells". Oncology Reports 29.1 (2013): 237-243.
Chicago
Lee, Y., Lee, L., Yang, C., Lin, A. M., Huang, Y., Hsu, C., Chen, M., Chi, C., Yin, P., Kuo, C., Liao, J., Lee, H."Norcantharidin suppresses cell growth and migration with enhanced anticancer activity of gefitinib and cisplatin in human non-small cell lung cancer cells". Oncology Reports 29, no. 1 (2013): 237-243. https://doi.org/10.3892/or.2012.2118
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