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Article

Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin

  • Authors:
    • Hongmin Che
    • Jianrong Song
    • Shiwen Guo
    • Weiwen Wang
    • Guodong Gao
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Second Affiliated Hospital, the Fourth Military Medical University, Xi'an 710038, P.R. China, Department of Neurosurgery, Baoji Municipal Central Hospital, Baoji, Shannxi 721008, P.R. China, Department of Neurosurgery, The First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, P.R. China
  • Pages: 1101-1107
    |
    Published online on: December 14, 2012
       https://doi.org/10.3892/or.2012.2187
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Abstract

Angiogenesis is crucial for the development and metastasis of human brain glioma. Based on our previous successful construction of a lentivirus-mediated alphastatin (an endogenous angiogenesis inhibitor) gene transfer system and our findings that alphastatin exhibited potent inhibitory effects on the migration and differentiation of human umbilical vein endothelial cell lines (HUVECs) induced by vascular endothelial growth factor (VEGF) or basic fibroblast growth factor (bFGF) in vitro, here, we investigated the effect of using lentiviral vectors to overexpress alphastatin in human glioma cells to show whether sustained long-term expression of alphastatin diminishes tumor growth in a xenograft glioma model. We found that the transduced glioma cells sustainedly secreted alphastatin, which did not affect the proliferative ability of the glioma cells. Furthermore, tumor xenografts treated with the recombinant lentivirus were significantly smaller compared to the control xenografts and vascularity within the treated tumors was evidently decreased. Our data suggest that stable expression of alphastatin inhibits human glioma growth by inhibiting angiogenesis, with a probable mechanism of suppressing the turnover of VE-cadherin membrane molecules.
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Copy and paste a formatted citation
Spandidos Publications style
Che H, Song J, Guo S, Wang W and Gao G: Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin. Oncol Rep 29: 1101-1107, 2013.
APA
Che, H., Song, J., Guo, S., Wang, W., & Gao, G. (2013). Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin. Oncology Reports, 29, 1101-1107. https://doi.org/10.3892/or.2012.2187
MLA
Che, H., Song, J., Guo, S., Wang, W., Gao, G."Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin". Oncology Reports 29.3 (2013): 1101-1107.
Chicago
Che, H., Song, J., Guo, S., Wang, W., Gao, G."Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin". Oncology Reports 29, no. 3 (2013): 1101-1107. https://doi.org/10.3892/or.2012.2187
Copy and paste a formatted citation
x
Spandidos Publications style
Che H, Song J, Guo S, Wang W and Gao G: Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin. Oncol Rep 29: 1101-1107, 2013.
APA
Che, H., Song, J., Guo, S., Wang, W., & Gao, G. (2013). Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin. Oncology Reports, 29, 1101-1107. https://doi.org/10.3892/or.2012.2187
MLA
Che, H., Song, J., Guo, S., Wang, W., Gao, G."Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin". Oncology Reports 29.3 (2013): 1101-1107.
Chicago
Che, H., Song, J., Guo, S., Wang, W., Gao, G."Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin". Oncology Reports 29, no. 3 (2013): 1101-1107. https://doi.org/10.3892/or.2012.2187
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