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Article

Diarylquinoline compounds induce autophagy-associated cell death by inhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngeal carcinoma cells

  • Authors:
    • Yuchen Cai
    • Zhihui Wan
    • Tiemin Sun
    • Yanxia Shi
    • Yueli Sun
    • Peiyu Huang
    • Su Li
    • Wenqi Jiang
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, P.R. China, Department of Medical Oncology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong, P.R. China, Shenyang Pharmaceutical University, Shenyang, Liaoning, P.R. China
  • Pages: 983-992
    |
    Published online on: December 24, 2012
       https://doi.org/10.3892/or.2012.2207
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Abstract

Diarylquinoline compounds are newly synthesized derivatives of the new anti-tuberculosis drug, TMC207. In this study, nine diarylquinoline compounds were screened for cytotoxic activity against human tumor cells, and their mechanisms of action were investigated. Among the nine compounds, STM-57 [N-((6-bromo-2-methoxyquinolin-3-yl)(phenyl)methl)-N-(3,4-dichlorophenyl)-3-(4 -methylpiperazin-1-yl)propanamide] showed potent cytotoxic activity. STM-57 induced caspase-independent cell death in the human nasopharyngeal carcinoma cell line, CNE-2. Further investigation showed that STM-57 induced autophagy, as determined with the increased expression of green fluorescent protein-light chain 3 (GFP-LC3) and increased LC3-II levels. STM-57 inhibited the phosphorylation of Akt and the mammalian target of rapamycin (mTOR) in CNE-2 cells. The intracellular calcium concentration and reactive oxygen species levels were increased in CNE-2 cells following treatment with STM-57, whereas the mitochondrial transmembrane potential (ΔΨm) and ATP concentrations were decreased.
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Copy and paste a formatted citation
Spandidos Publications style
Cai Y, Wan Z, Sun T, Shi Y, Sun Y, Huang P, Li S and Jiang W: Diarylquinoline compounds induce autophagy-associated cell death by inhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngeal carcinoma cells. Oncol Rep 29: 983-992, 2013.
APA
Cai, Y., Wan, Z., Sun, T., Shi, Y., Sun, Y., Huang, P. ... Jiang, W. (2013). Diarylquinoline compounds induce autophagy-associated cell death by inhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngeal carcinoma cells. Oncology Reports, 29, 983-992. https://doi.org/10.3892/or.2012.2207
MLA
Cai, Y., Wan, Z., Sun, T., Shi, Y., Sun, Y., Huang, P., Li, S., Jiang, W."Diarylquinoline compounds induce autophagy-associated cell death by inhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngeal carcinoma cells". Oncology Reports 29.3 (2013): 983-992.
Chicago
Cai, Y., Wan, Z., Sun, T., Shi, Y., Sun, Y., Huang, P., Li, S., Jiang, W."Diarylquinoline compounds induce autophagy-associated cell death by inhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngeal carcinoma cells". Oncology Reports 29, no. 3 (2013): 983-992. https://doi.org/10.3892/or.2012.2207
Copy and paste a formatted citation
x
Spandidos Publications style
Cai Y, Wan Z, Sun T, Shi Y, Sun Y, Huang P, Li S and Jiang W: Diarylquinoline compounds induce autophagy-associated cell death by inhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngeal carcinoma cells. Oncol Rep 29: 983-992, 2013.
APA
Cai, Y., Wan, Z., Sun, T., Shi, Y., Sun, Y., Huang, P. ... Jiang, W. (2013). Diarylquinoline compounds induce autophagy-associated cell death by inhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngeal carcinoma cells. Oncology Reports, 29, 983-992. https://doi.org/10.3892/or.2012.2207
MLA
Cai, Y., Wan, Z., Sun, T., Shi, Y., Sun, Y., Huang, P., Li, S., Jiang, W."Diarylquinoline compounds induce autophagy-associated cell death by inhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngeal carcinoma cells". Oncology Reports 29.3 (2013): 983-992.
Chicago
Cai, Y., Wan, Z., Sun, T., Shi, Y., Sun, Y., Huang, P., Li, S., Jiang, W."Diarylquinoline compounds induce autophagy-associated cell death by inhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngeal carcinoma cells". Oncology Reports 29, no. 3 (2013): 983-992. https://doi.org/10.3892/or.2012.2207
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