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Article

Podoplanin overexpression in human mesothelioma cell lines enhances the tumorigenic phenotype

  • Authors:
    • Ei Yamaki
    • Toshiki Yajima
    • Takayuki Kosaka
    • Akira Mogi
    • Shigebumi Tanaka
    • Hiroyuki Kuwano
  • View Affiliations / Copyright

    Affiliations: Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan
  • Pages: 932-940
    |
    Published online on: January 4, 2013
       https://doi.org/10.3892/or.2013.2225
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Abstract

Podoplanin, a small type I integral membrane mucin-type sialoglycoprotein, serves as a useful marker for diagnosing malignant pleural mesothelioma (MPM); however, the physiological function of podoplanin in mesothelioma cells is not known. To elucidate the role of podoplanin in the pathogenesis of MPM, we generated two mesothelioma cell lines (PODO1 and PODO2) that stably express high levels of podoplanin. Although PODO1 cells proliferated to the same extent in culture or in nude mice, the survival rate of the mice was significantly reduced compared with that of the controls. We demonstrated that PODO1 and PODO2 cells had increased invasive ability in in vitro assays and induced upregulation of matrix metalloproteinase-1. PODO1 and PODO2 cultures could not be induced to undergo apoptosis when starved or treated with cis-diamminedichloroplatinum(II) (CDDP) compared with the controls. Moreover, silencing of podoplanin expression using RNA interference restored the ability of CDDP to induce apoptosis. Consistent with their growth properties, we detected constitutive activation of extracellular signal-regulated kinase in PODO1 and PODO2 cultures. These findings suggest that constitutive expression of podoplanin contributes to the invasive growth properties of mesothelioma cells and their resistance to apoptosis. Moreover, our data suggest that podoplanin or components of its signaling pathway, or both, may serve as important targets for developing novel treatments for MPM.
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Copy and paste a formatted citation
Spandidos Publications style
Yamaki E, Yajima T, Kosaka T, Mogi A, Tanaka S and Kuwano H: Podoplanin overexpression in human mesothelioma cell lines enhances the tumorigenic phenotype. Oncol Rep 29: 932-940, 2013.
APA
Yamaki, E., Yajima, T., Kosaka, T., Mogi, A., Tanaka, S., & Kuwano, H. (2013). Podoplanin overexpression in human mesothelioma cell lines enhances the tumorigenic phenotype. Oncology Reports, 29, 932-940. https://doi.org/10.3892/or.2013.2225
MLA
Yamaki, E., Yajima, T., Kosaka, T., Mogi, A., Tanaka, S., Kuwano, H."Podoplanin overexpression in human mesothelioma cell lines enhances the tumorigenic phenotype". Oncology Reports 29.3 (2013): 932-940.
Chicago
Yamaki, E., Yajima, T., Kosaka, T., Mogi, A., Tanaka, S., Kuwano, H."Podoplanin overexpression in human mesothelioma cell lines enhances the tumorigenic phenotype". Oncology Reports 29, no. 3 (2013): 932-940. https://doi.org/10.3892/or.2013.2225
Copy and paste a formatted citation
x
Spandidos Publications style
Yamaki E, Yajima T, Kosaka T, Mogi A, Tanaka S and Kuwano H: Podoplanin overexpression in human mesothelioma cell lines enhances the tumorigenic phenotype. Oncol Rep 29: 932-940, 2013.
APA
Yamaki, E., Yajima, T., Kosaka, T., Mogi, A., Tanaka, S., & Kuwano, H. (2013). Podoplanin overexpression in human mesothelioma cell lines enhances the tumorigenic phenotype. Oncology Reports, 29, 932-940. https://doi.org/10.3892/or.2013.2225
MLA
Yamaki, E., Yajima, T., Kosaka, T., Mogi, A., Tanaka, S., Kuwano, H."Podoplanin overexpression in human mesothelioma cell lines enhances the tumorigenic phenotype". Oncology Reports 29.3 (2013): 932-940.
Chicago
Yamaki, E., Yajima, T., Kosaka, T., Mogi, A., Tanaka, S., Kuwano, H."Podoplanin overexpression in human mesothelioma cell lines enhances the tumorigenic phenotype". Oncology Reports 29, no. 3 (2013): 932-940. https://doi.org/10.3892/or.2013.2225
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