Method for the validation of immunohistochemical staining using SCID mouse xenografts: Expression of CD40 and CD154 in human non-small cell lung cancer

Ishikawa,Keidai; Miyamoto,Masaki; Yoshioka,Tatsuya; Kadoya,Masatoshi; Li,Li; Mishra,Roshan; Ichinokawa,Kazuomi; Shoji,Yasuhito; Matsumura,Yoshiyuki; Hida,Yasuhiro; Kaga,Kichizo; Kato,Tatsuya; Kaji,Mitsuhito; Ohbuchi,Toshiro; Itoh,Tomoo; Dosaka-Akita,Hirotoshi; Matsui,Yoshiro; Hirano,Satoshi

doi:10.3892/or.2013.2275

Method for the validation of immunohistochemical staining using SCID mouse xenografts: Expression of CD40 and CD154 in human non-small cell lung cancer

Authors:
- Keidai Ishikawa
- Masaki Miyamoto
- Tatsuya Yoshioka
- Masatoshi Kadoya
- Li Li
- Roshan Mishra
- Kazuomi Ichinokawa
- Yasuhito Shoji
- Yoshiyuki Matsumura
- Yasuhiro Hida
- Kichizo Kaga
- Tatsuya Kato
- Mitsuhito Kaji
- Toshiro Ohbuchi
- Tomoo Itoh
- Hirotoshi Dosaka-Akita
- Yoshiro Matsui
- Satoshi Hirano
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Affiliations: Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan, Department of Cardiovascular and Thoracic Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan, Department of Thoracic Surgery, Sapporo-Minamisanjo Hospital, Sapporo, Hokkaido 060-0063, Japan, Department of Thoracic Surgery, Minami-ichijo Hospital, Sapporo, Hokkaido 060-0061, Japan, Department of Diagnostic Pathology, Kobe University Hospital, Kobe, Hyogo 650-0017, Japan, Department of Medical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan
Published online on: February 5, 2013 https://doi.org/10.3892/or.2013.2275
Pages: 1315-1321
Copyright: © Ishikawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

This report proposes a concept for the standardization of immunohistochemical evaluation. Immunohistochemical staining has several problems associated with the sensitivity of the technical process and standardization of the assessment of potent staining. We provided data focusing on this concept through immunostaining for CD154 in non-small cell lung cancer (NSCLC). We used two types of anti-CD154 antibody as primary antibodies in immunohistochemical staining, as previously reported. Western blot analysis confirmed strong CD154 expression in the cultured cell line PC10, but not in LK2. We also assessed CD154 expression in SCID mouse xenografts of these cell lines. SCID xenograft data on western blot analysis were consistent with those of cultured cell lines. These xenografts could thus be used as positive or negative tissue controls for CD154 immunostaining. Primary antibodies should therefore be confirmed as recognizing target lesions, while control tissue specimens should be objectively confirmed as having target products using another experimental method. Our method would allow results to be unified at more than one laboratory and could act as an objective control assessment method in immunohistochemistry.

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1	Banchereau J, Bazan F, Blanchard D, et al: The CD40 antigen and its ligand. Annu Rev Immunol. 12:881–922. 1994. View Article : Google Scholar : PubMed/NCBI
2	Smith CA, Farrah T and Goodwin RG: The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death. Cell. 76:959–962. 1994. View Article : Google Scholar : PubMed/NCBI
3	Paulie S, Ehlin-Henriksson B, Mellstedt H, Koho H, Ben-Aissa H and Perlman BA: p50 surface antigen restricted to human urinary bladder carcinomas and B lymphocytes. Cancer Immunol Immunother. 20:23–28. 1985. View Article : Google Scholar : PubMed/NCBI
4	Aldderson MR, Armitage R, Tough TW, Strockbine L, Fanslow WC and Spriggs M: CD40 expression by human monocytes: regulation by cytokines and activation of monocytes by the ligand for CD4. Exp Med. 178:669–674. 1993. View Article : Google Scholar : PubMed/NCBI
5	Caux C, Massacrier C, Vanbervliet B, et al: Activation of human dendritic cells through CD40 cross-linking. J Exp Med. 180:1263–1272. 1994. View Article : Google Scholar : PubMed/NCBI
6	Yellin MJ, Winikoff S, Fortune SM, et al: Ligation of CD40 on fibroblast induces CD54 (ICAM-1) and CD106 (VCAM-1) up-regulation and IL-6 production and proliferation. J Leukocyt Biol. 58:209–216. 1995.PubMed/NCBI
7	Zong YS, Lin H, Choy DTK, et al: Nasopharyngeal carcinoma and lymphoinfiltration. Oncology. 48:290–296. 1991. View Article : Google Scholar : PubMed/NCBI
8	Galy AHM and Spitz H: CD40 is functionally expressed on human thymic epithelial cells. J Immunol. 149:775–782. 1992.PubMed/NCBI
9	Pammer J, Weninger W, Mazal PR, Horvat R and Tschachler E: Expression of the CD40 antigen on normal endothelial cells and in benign and malignant tumours of vascular origin. Histopathology. 29:517–524. 1996. View Article : Google Scholar : PubMed/NCBI
10	Armitage RJ, Fanslow WC, Stockbine L, et al: Molecular and biological characterization of a murine ligand for CD40. Nature. 357:80–82. 1992. View Article : Google Scholar : PubMed/NCBI
11	Gauchat JF, Henchoz S, Mazzei G, et al: Induction of human IgE synthesis in B cells by mast cells and basophils. Nature. 365:340–343. 1993. View Article : Google Scholar : PubMed/NCBI
12	Banchereau J, de Paoli P, Valle A, et al: Long-term human B cell lines dependent on interleukin-4 and antibody to CD40. Science. 251:70–72. 1991. View Article : Google Scholar : PubMed/NCBI
13	Liu YJ, Mason DY, Johnson GD, et al: Germinal center cells express bcl-2 protein after activation by signals which prevent their entry into apoptosis. Eur J Immunol. 21:1905–1910. 1991. View Article : Google Scholar : PubMed/NCBI
14	Choi MSK, Boise LH, Gottschalk AR, Quintans J, Thompson CB and Klaus GGB: The role of bcl-xl-mediated rescue from anti-μ-induced apoptosis in WEHI-231 B lymphoma cells. Eur J Immunol. 25:1352–1357. 1995.
15	Ludewig B, Graf D, Gelderblom HR, Becker Y, Kroczek RA and Pauli G: Spontaneous apoptosis of dendritic cells is efficiency inhibited by TRAP (CD40-ligand) and TNF-α, but strongly enhanced by interleukin-10. Eur J Immunol. 25:1943–1950. 1995.PubMed/NCBI
16	Tong AW, Papayoti MH, Netto G, et al: Growth-inhibitory effects of CD40 ligand (CD154) and its endogenous expression in human breast cancer. Clin Cancer Res. 3:691–703. 2001.PubMed/NCBI
17	Costello A, Rey-Hipolito C, Patel A, et al: Thyroid cancers express CD-40 and CD-40 ligand: cancers that express CD-40 ligand may have a greater risk of recurrence in young patients. Thyroid. 2:105–113. 2005. View Article : Google Scholar : PubMed/NCBI
18	Campean V, Neureiter D, Nonnast-Daniel B, Garlichs C, Gross ML and Amann K: CD40-CD154 expression in calcified and non calcified coronary lesions of patients with chronic renal failure. Atherosclerosis. 190:155–166. 2007. View Article : Google Scholar : PubMed/NCBI
19	Yamada M, Shiroko T, Kawaguchi Y, et al: CD40-CD40 ligand (CD154) engagement is required but not sufficient for modulating MHC class I, ICAM-1 and Fas expression and proliferation of human non-small cell lung tumors. Int J Cancer. 92:589–599. 2001. View Article : Google Scholar : PubMed/NCBI
20	Ishikawa K, Miyamoto M, Yoshioka T, et al: Up-regulation of CD40 with juxtacrine activity in human nonsmall lung cancer cells correlates with poor prognosis. Cancer. 113:530–541. 2008. View Article : Google Scholar : PubMed/NCBI
21	Bajorath J, Seyama K, Nonoyama S, Ochs HD and Aruffo A: Classification of mutations in the human CD40 ligand, gp39, that is associated with X-linked hyper IgM syndrome. Protein Sci. 5:531–534. 1996. View Article : Google Scholar : PubMed/NCBI
22	Fujimoto T, Nakamura T, Nishimura Y, et al: Up-regulation of interleukin-12 receptor expression in peripheral blood mononuclear cells of patients with HTLV-I-associated myelopathy/tropical spastic paraparesis. J Neurol Sci. 196:21–26. 2002. View Article : Google Scholar
23	Balashov KE, Smith DR, Khoury SJ, Hafler DA and Weiner HL: Increased interleukin 12 production in progressive multiple sclerosis: Induction by activated CD4⁺ T cells via CD40 ligand. Proc Natl Acad Sci USA. 94:599–603. 1997. View Article : Google Scholar : PubMed/NCBI
24	Eriksson T, Frisk T, Gray SG, et al: Methylation changes in the human IGF2 p3 promoter parallel IGF2 expression in the primary tumor, established cell line, and xenograft of a human hepatoblastoma. Exp Cell Res. 270:88–95. 2001. View Article : Google Scholar : PubMed/NCBI
25	Hellings PW, Kasran A, Bullens D, et al: IL-10- and IL-12-independent down-regulation of allergic sensitization by stimulation of CD40 signaling. J Immunol. 177:5138–5144. 2006. View Article : Google Scholar : PubMed/NCBI