Method for the validation of immunohistochemical staining using SCID mouse xenografts: Expression of CD40 and CD154 in human non-small cell lung cancer

Ishikawa,Keidai; Miyamoto,Masaki; Yoshioka,Tatsuya; Kadoya,Masatoshi; Li,Li; Mishra,Roshan; Ichinokawa,Kazuomi; Shoji,Yasuhito; Matsumura,Yoshiyuki; Hida,Yasuhiro; Kaga,Kichizo; Kato,Tatsuya; Kaji,Mitsuhito; Ohbuchi,Toshiro; Itoh,Tomoo; Dosaka-Akita,Hirotoshi; Matsui,Yoshiro; Hirano,Satoshi

doi:10.3892/or.2013.2275

April 2013 Volume 29 Issue 4

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April 2013 Volume 29 Issue 4

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Article Open Access

Method for the validation of immunohistochemical staining using SCID mouse xenografts: Expression of CD40 and CD154 in human non-small cell lung cancer

Authors:
- Keidai Ishikawa
- Masaki Miyamoto
- Tatsuya Yoshioka
- Masatoshi Kadoya
- Li Li
- Roshan Mishra
- Kazuomi Ichinokawa
- Yasuhito Shoji
- Yoshiyuki Matsumura
- Yasuhiro Hida
- Kichizo Kaga
- Tatsuya Kato
- Mitsuhito Kaji
- Toshiro Ohbuchi
- Tomoo Itoh
- Hirotoshi Dosaka-Akita
- Yoshiro Matsui
- Satoshi Hirano
View Affiliations / Copyright

Affiliations: Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan, Department of Cardiovascular and Thoracic Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan, Department of Thoracic Surgery, Sapporo-Minamisanjo Hospital, Sapporo, Hokkaido 060-0063, Japan, Department of Thoracic Surgery, Minami-ichijo Hospital, Sapporo, Hokkaido 060-0061, Japan, Department of Diagnostic Pathology, Kobe University Hospital, Kobe, Hyogo 650-0017, Japan, Department of Medical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan

Copyright: © Ishikawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 1315-1321
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Published online on: February 5, 2013

https://doi.org/10.3892/or.2013.2275
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Abstract

This report proposes a concept for the standardization of immunohistochemical evaluation. Immunohistochemical staining has several problems associated with the sensitivity of the technical process and standardization of the assessment of potent staining. We provided data focusing on this concept through immunostaining for CD154 in non-small cell lung cancer (NSCLC). We used two types of anti-CD154 antibody as primary antibodies in immunohistochemical staining, as previously reported. Western blot analysis confirmed strong CD154 expression in the cultured cell line PC10, but not in LK2. We also assessed CD154 expression in SCID mouse xenografts of these cell lines. SCID xenograft data on western blot analysis were consistent with those of cultured cell lines. These xenografts could thus be used as positive or negative tissue controls for CD154 immunostaining. Primary antibodies should therefore be confirmed as recognizing target lesions, while control tissue specimens should be objectively confirmed as having target products using another experimental method. Our method would allow results to be unified at more than one laboratory and could act as an objective control assessment method in immunohistochemistry.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Method for the validation of immunohistochemical staining using SCID mouse xenografts: Expression of CD40 and CD154 in human non-small cell lung cancer

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