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Article

Antiproliferative activity of gambogic acid isolated from Garcinia hanburyi in Hep3B and Huh7 cancer cells

  • Authors:
    • Parry Ngan Hon Lee
    • Wing Shing Ho
  • View Affiliations / Copyright

    Affiliations: School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China
  • Pages: 1744-1750
    |
    Published online on: February 19, 2013
       https://doi.org/10.3892/or.2013.2291
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Abstract

The anticancer activities of gambogic acid (GA) on two hepatocellular carcinoma cells with either p53 deletion (Hep3B) or p53 mutation (Huh7) were investigated in the present study. GA inhibited the growth of Hep3B and Huh7 through similar apoptotic pathways. After treatment of Hep3B and Huh7 with GA for 24 h, the IC50 was determined for both cell lines at 1.8 and 2.2 µM, respectively. The results showed that both cancer cells underwent morphological changes and DNA fragmentation. GA induced apoptosis in the two cell lines through caspases-3/7, -8 and -9 in the mitochondrial pathway. The results suggest that both the caspases in the extrinsic death receptor pathway and the mitochondrial-dependent pathway are involved in the GA-induced cell apoptosis. The inhibitory effects of GA on Hep3B and Huh7 are independent of p53-associated pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Lee PN and Ho WS: Antiproliferative activity of gambogic acid isolated from Garcinia hanburyi in Hep3B and Huh7 cancer cells. Oncol Rep 29: 1744-1750, 2013.
APA
Lee, P.N., & Ho, W.S. (2013). Antiproliferative activity of gambogic acid isolated from Garcinia hanburyi in Hep3B and Huh7 cancer cells. Oncology Reports, 29, 1744-1750. https://doi.org/10.3892/or.2013.2291
MLA
Lee, P. N., Ho, W. S."Antiproliferative activity of gambogic acid isolated from Garcinia hanburyi in Hep3B and Huh7 cancer cells". Oncology Reports 29.5 (2013): 1744-1750.
Chicago
Lee, P. N., Ho, W. S."Antiproliferative activity of gambogic acid isolated from Garcinia hanburyi in Hep3B and Huh7 cancer cells". Oncology Reports 29, no. 5 (2013): 1744-1750. https://doi.org/10.3892/or.2013.2291
Copy and paste a formatted citation
x
Spandidos Publications style
Lee PN and Ho WS: Antiproliferative activity of gambogic acid isolated from Garcinia hanburyi in Hep3B and Huh7 cancer cells. Oncol Rep 29: 1744-1750, 2013.
APA
Lee, P.N., & Ho, W.S. (2013). Antiproliferative activity of gambogic acid isolated from Garcinia hanburyi in Hep3B and Huh7 cancer cells. Oncology Reports, 29, 1744-1750. https://doi.org/10.3892/or.2013.2291
MLA
Lee, P. N., Ho, W. S."Antiproliferative activity of gambogic acid isolated from Garcinia hanburyi in Hep3B and Huh7 cancer cells". Oncology Reports 29.5 (2013): 1744-1750.
Chicago
Lee, P. N., Ho, W. S."Antiproliferative activity of gambogic acid isolated from Garcinia hanburyi in Hep3B and Huh7 cancer cells". Oncology Reports 29, no. 5 (2013): 1744-1750. https://doi.org/10.3892/or.2013.2291
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