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Article

miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9

  • Authors:
    • Bo Li
    • Yan Song
    • Tong-Jun Liu
    • You-Bin Cui
    • Yang Jiang
    • Zhong-Shi Xie
    • Shu-Li Xie
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal and Anal Surgery, The Third Affiliated Hospital of Jilin University, Changchun, Jilin, P.R. China, Department of Thoracic Surgery, The First Affiliated Hospital of Jilin University, Changchun, Jilin, P.R. China, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Jilin University, Changchun, Jilin, P.R. China
  • Pages: 1932-1938
    |
    Published online on: February 22, 2013
       https://doi.org/10.3892/or.2013.2300
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Abstract

Emerging evidence has demonstrated the altered expression of mRNAs in cancer development and progression. In this study, the precise role of miRNA-22 (miR-22) in colon cancer cells was investigated. Upon transfection with a miR-22 expression vector, the viability of HCT-116 human colon cancer cells was significantly reduced and tumor cell migration and invasion capacity were also suppressed. Computational in silico analysis predicted that T-cell lymphoma invasion and metastasis 1 (TIAM1) is a target gene of miR-22. This was confirmed by qRT-PCR and western blotting, which showed that miR-22 expression inhibited TIAM1 mRNA and protein expression, respectively. In addition, the expression of pro-invasive gene matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) and pro-angiogenic protein vascular endothelial growth factor (VEGF) were also reduced by miR-22 expression. Collectively, these data suggest that miR-22 may act as a tumor suppressor in colon cancer, most likely by targeting TIAM1 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Li B, Song Y, Liu T, Cui Y, Jiang Y, Xie Z and Xie S: miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9. Oncol Rep 29: 1932-1938, 2013.
APA
Li, B., Song, Y., Liu, T., Cui, Y., Jiang, Y., Xie, Z., & Xie, S. (2013). miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9. Oncology Reports, 29, 1932-1938. https://doi.org/10.3892/or.2013.2300
MLA
Li, B., Song, Y., Liu, T., Cui, Y., Jiang, Y., Xie, Z., Xie, S."miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9". Oncology Reports 29.5 (2013): 1932-1938.
Chicago
Li, B., Song, Y., Liu, T., Cui, Y., Jiang, Y., Xie, Z., Xie, S."miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9". Oncology Reports 29, no. 5 (2013): 1932-1938. https://doi.org/10.3892/or.2013.2300
Copy and paste a formatted citation
x
Spandidos Publications style
Li B, Song Y, Liu T, Cui Y, Jiang Y, Xie Z and Xie S: miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9. Oncol Rep 29: 1932-1938, 2013.
APA
Li, B., Song, Y., Liu, T., Cui, Y., Jiang, Y., Xie, Z., & Xie, S. (2013). miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9. Oncology Reports, 29, 1932-1938. https://doi.org/10.3892/or.2013.2300
MLA
Li, B., Song, Y., Liu, T., Cui, Y., Jiang, Y., Xie, Z., Xie, S."miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9". Oncology Reports 29.5 (2013): 1932-1938.
Chicago
Li, B., Song, Y., Liu, T., Cui, Y., Jiang, Y., Xie, Z., Xie, S."miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9". Oncology Reports 29, no. 5 (2013): 1932-1938. https://doi.org/10.3892/or.2013.2300
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