Genetic inhibition of vascular endothelial growth factor receptor-1 significantly inhibits the migration and proliferation of leukemia cells and increases their sensitivity to chemotherapy

  • Authors:
    • Bing Xiu
    • Wenjun Zhang
    • Binbin Huang
    • Jingde Chen
    • Huina Lu
    • Jianfei Fu
    • Hong Xiong
    • Aibin Liang
  • View Affiliations

  • Published online on: March 13, 2013     https://doi.org/10.3892/or.2013.2348
  • Pages: 2030-2038
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Little is known about the role of vascular endothelial growth factor (VEGF) receptor-1 (VEGFR-1) in acute leukemia. In this study, using real-time PCR and ELISA, we found that VEGF and VEGFR-1 are highly expressed in U937 leukemia cells and primary leukemia cells (M4/M5 subtypes), which are associated with an increased migration rate and extramedullary disease. In order to elucidate the role of VEGFR-1 in acute leukemia, we used a lentivirus-mediated shRNA expression system to specifically inhibit VEGFR-1 expression in the U937 cell line. In addition, a series of in vitro experiments were conducted, including cell proliferation and migration assays and drug treatments. Our results showed that shRNA reduced the proliferation and migration of U937 cells. RNA interference targeting VEGFR-1 in combination with bevacizumab did not exert synergistic antitumor effects. However, shRNA enhanced the sensitivity of the U937 cells to cytarabine by decreasing the IC50 of cytarabine, reducing the number of cells in the S phase and suppressing the expression of the survivin gene. Taken together, these results suggest that VEGFR-1 interference may serve as a novel antitumor therapeutic strategy for the treatment of leukemia.
View Figures
View References

Related Articles

Journal Cover

May 2013
Volume 29 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xiu B, Zhang W, Huang B, Chen J, Lu H, Fu J, Xiong H and Liang A: Genetic inhibition of vascular endothelial growth factor receptor-1 significantly inhibits the migration and proliferation of leukemia cells and increases their sensitivity to chemotherapy. Oncol Rep 29: 2030-2038, 2013
APA
Xiu, B., Zhang, W., Huang, B., Chen, J., Lu, H., Fu, J. ... Liang, A. (2013). Genetic inhibition of vascular endothelial growth factor receptor-1 significantly inhibits the migration and proliferation of leukemia cells and increases their sensitivity to chemotherapy. Oncology Reports, 29, 2030-2038. https://doi.org/10.3892/or.2013.2348
MLA
Xiu, B., Zhang, W., Huang, B., Chen, J., Lu, H., Fu, J., Xiong, H., Liang, A."Genetic inhibition of vascular endothelial growth factor receptor-1 significantly inhibits the migration and proliferation of leukemia cells and increases their sensitivity to chemotherapy". Oncology Reports 29.5 (2013): 2030-2038.
Chicago
Xiu, B., Zhang, W., Huang, B., Chen, J., Lu, H., Fu, J., Xiong, H., Liang, A."Genetic inhibition of vascular endothelial growth factor receptor-1 significantly inhibits the migration and proliferation of leukemia cells and increases their sensitivity to chemotherapy". Oncology Reports 29, no. 5 (2013): 2030-2038. https://doi.org/10.3892/or.2013.2348