Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
June 2013 Volume 29 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June 2013 Volume 29 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts

  • Authors:
    • Andrew J. Rainbow
    • Natalie J. Zacal
    • Derrik M. Leach
  • View Affiliations / Copyright

    Affiliations: Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
  • Pages: 2493-2497
    |
    Published online on: March 22, 2013
       https://doi.org/10.3892/or.2013.2358
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Many reports have linked oxidative damage to DNA and the associated avoidance and/or repair processes to carcinogenesis, ageing and neurodegeneration. Cancer incidence increases with age and there is evidence that oxidative stress plays a role in human ageing and neurodegeneration. Several reports have suggested that the accumulation of unrepaired DNA lesions plays a causal role in mammalian ageing. Since base excision repair (BER) is the main pathway for the repair of oxidative DNA lesions, the relationship of BER to human ageing and carcinogenesis is of considerable interest. The aim of the present study was to examine the relationship between donor age and increasing time of cells in tissue culture and the repair of oxidative DNA damage in primary human skin fibroblasts. Methylene blue (MB) acts as a photosensitizer and after excitation by visible light (VL) produces reactive oxygen species that result in oxidative damage to DNA. MB+VL produce predominantly 8-hydroxyguanine as well as other single base modifications in DNA that are repaired by BER. We used host cell reactivation (HCR) of a non-replicating recombinant human adenovirus, Ad5CMVlacZ, which expresses the β-galactosidase (β-gal) reporter gene, to measure BER of MB+VL-damaged DNA. HCR of β-gal activity for the MB+VL-treated reporter gene was examined in 10 fibroblast strains from normal donors of ages 2 to 82. The effect of cell passage number on HCR was also examined in human skin fibroblasts from 2 normal donors. We found a significant reduction in HCR with increasing cell passage number, indicating that BER decreases with increasing time of cells grown in tissue culture. We also found a significant correlation of donor age with HCR of the MB+VL-treated reporter gene for high passage number, but not for low passage number fibroblasts. The present study provides evidence that a decrease in BER of oxidatively damaged DNA may play a role in carcinogenesis, ageing and neurodegeneration.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Maynard S, Schurman SH, Harboe C, de Souza-Pinto NC and Bohr VA: Base excision repair of oxidative DNA damage and association with cancer and aging. Carcinogenesis. 30:2–10. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Tuo J, Jaruga P, Rodriguez H, Bohr VA and Dizdaroglu M: Primary fibroblasts of Cockayne syndrome patients are defective in cellular repair of 8-hydroxyguanine and 8-hydroxyadenine resulting from oxidative stress. FASEB J. 17:668–674. 2003. View Article : Google Scholar

3 

Chen SK, Hsieh WA, Tsai MH, Chen CC, Hong AI, Wei YH and Chang WP: Age-associated decrease of oxidative repair enzymes, human 8-oxoguanine DNA glycosylases (hOgg1), in human aging. J Radiat Res. 44:31–35. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Szczesny B, Hazra TK, Papaconstantinou J, Mitra S and Boldogh I: Age-dependent deficiency in import of mitochondrial DNA glycosylases required for repair of oxidatively damaged bases. Proc Natl Acad Sci USA. 100:10670–10675. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Cabelof DC, Raffoul JJ, Ge Y, Van Remmen H, Matherly LH and Heydari AR: Age-related loss of the DNA repair response following exposure to oxidative stress. J Gerontol A Biol Sci Med Sci. 61:427–434. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Ksiazek K, Piatek K and Witowski J: Impaired response to oxidative stress in senescent cells may lead to accumulation of DNA damage in mesothelial cells from aged donors. Biochem Biophys Res Commun. 373:335–339. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Yuan H, Kaneko T and Matsuo M: Increased susceptibility of late passage human diploid fibroblasts to oxidative stress. Exp Gerontol. 31:465–474. 1996. View Article : Google Scholar : PubMed/NCBI

8 

Matsuo M, Ikeda H, Sugihara T, Horiike S, Okano Y and Masaki H: Resistance of cultured human skin fibroblasts from old and young donors to oxidative stress and their glutathione peroxidase activity. Gerontology. 50:193–199. 2004. View Article : Google Scholar

9 

Kaneko T, Tahara S, Taguchi T and Kondo H: Accumulation of oxidative DNA damage, 8-oxo-2′-deoxyguanosine, and change of repair systems during in vitro cellular aging of cultured human skin fibroblasts. Mutat Res. 487:19–30. 2001.

10 

Kassam SN and Rainbow AJ: Deficient base excision repair of oxidative DNA damage induced by methylene blue plus visible light in xeroderma pigmentosum group C fibroblasts. Biochem Biophys Res Commun. 359:1004–1009. 2007. View Article : Google Scholar

11 

Kassam SN and Rainbow AJ: UV-inducible base excision repair of oxidative damaged DNA in human cells. Mutagenesis. 24:75–83. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Leach DM and Rainbow AJ: Early host cell reactivation of an oxidatively damaged adenovirus-encoded reporter gene requires the Cockayne syndrome proteins CSA and CSB. Mutagenesis. 26:315–321. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Pitsikas P, Lee D and Rainbow AJ: Reduced host cell reactivation of oxidative DNA damage in human cells deficient in the mismatch repair gene hMSH2. Mutagenesis. 22:235–243. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Addison CL, Hitt M, Kunsken D and Graham FL: Comparison of the human versus murine cytomegalovirus immediate early gene promoters for transgene expression by adenoviral vectors. J Gen Virol. 78:1653–1661. 1997.PubMed/NCBI

15 

Slamenova D, Kuboskova K, Horvathova and Robichova S: Rosemary-stimulated reduction of DNA strand breaks and FPG-sensitive sites in mammalian cells treated with H2O2 or visible light-excited methylene blue. Cancer Lett. 177:145–153. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Floyd RA, West MS, Eneff KL and Schneider JE: Methylene blue plus light mediates 8-hydroxyguanine formation in DNA. Arch Biochem Biophys. 273:106–111. 1989. View Article : Google Scholar : PubMed/NCBI

17 

Tuite EM and Kelly JM: Photochemical interactions of methylene blue and analogues with DNA and other biological substrates. J Photochem Photobiol B. 21:103–124. 1993.

18 

Graham FL and Prevec L: Manipulation of adenovirus vectors. Methods Mol Biol. 7:109–128. 1991.PubMed/NCBI

19 

Pitsikas P, Francis MA and Rainbow AJ: Enhanced host cell reactivation of a UV-damaged reporter gene in pre-UV-treated cells is delayed in Cockayne syndrome cells. J Photochem Photobiol B. 81:89–97. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Takahashi Y, Moriwaki S, Sugiyama Y, Endo Y, Yamazaki K, Mori T, Takigawa M and Inoue S: Decreased gene expression responsible for post-ultraviolet DNA repair synthesis in aging: a possible mechanism of age-related reduction in DNA repair capacity. J Invest Dermatol. 124:435–442. 2005. View Article : Google Scholar

21 

Merkle TJ, O’Brien K, Brooks PJ, Tarone RE and Robbin JH: DNA repair in human fibroblasts, as reflected by host-cell reactivation of a transfected UV-irradiated luciferase gene, is not related to donor age. Mutat Res. 554:9–17. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Rainbow A , Zacal NJ and Leach DM: Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts. Oncol Rep 29: 2493-2497, 2013.
APA
Rainbow, A. ., Zacal, N.J., & Leach, D.M. (2013). Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts. Oncology Reports, 29, 2493-2497. https://doi.org/10.3892/or.2013.2358
MLA
Rainbow, A. ., Zacal, N. J., Leach, D. M."Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts". Oncology Reports 29.6 (2013): 2493-2497.
Chicago
Rainbow, A. ., Zacal, N. J., Leach, D. M."Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts". Oncology Reports 29, no. 6 (2013): 2493-2497. https://doi.org/10.3892/or.2013.2358
Copy and paste a formatted citation
x
Spandidos Publications style
Rainbow A , Zacal NJ and Leach DM: Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts. Oncol Rep 29: 2493-2497, 2013.
APA
Rainbow, A. ., Zacal, N.J., & Leach, D.M. (2013). Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts. Oncology Reports, 29, 2493-2497. https://doi.org/10.3892/or.2013.2358
MLA
Rainbow, A. ., Zacal, N. J., Leach, D. M."Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts". Oncology Reports 29.6 (2013): 2493-2497.
Chicago
Rainbow, A. ., Zacal, N. J., Leach, D. M."Reduced host cell reactivation of oxidatively damaged DNA in ageing human fibroblasts". Oncology Reports 29, no. 6 (2013): 2493-2497. https://doi.org/10.3892/or.2013.2358
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team