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Article

MGr1-Ag promotes invasion and bone metastasis of small-cell lung cancer in vitro and in vivo

  • Authors:
    • Feng Zhang
    • Yanxia Wang
    • Min Xu
    • Haiying Dong
    • Na Liu
    • Jing Zhou
    • Hailin Pang
    • Ningqiang Ma
    • Ning Zhang
    • Yanlin Pei
    • Helong Zhang
    • Lili Liu
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, P.R. China, Department of Pathology and Pathophysiology, The Fourth Military Medical University, Xi'an 710032, P.R. China
  • Pages: 2283-2290
    |
    Published online on: April 9, 2013
       https://doi.org/10.3892/or.2013.2396
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Abstract

Bone metastasis of small-cell lung cancer (SCLC) usually occurs early in the progression of the disease. However, the molecular mechanism underlying bone metastasis is largely unknown. MGr1-Ag, a multifunction protein, has been suggested to play important roles in cell growth, differentiation and migration. In our present study, MGr1-Ag was found to be highly expressed in bone-metastatic SCLC cells (SBC-5 cell line) as compared with the expression in cells without bone-metastatic ability (SBC-3 cell line). Therefore, we hypothesized that MGr1-Ag is involved in bone metastasis of SCLC. Using a sense vector to upregulate MGr1-Ag expression in SBC-3 cells, we found that forced overexpression of MGr1-Ag enhanced cell invasion and migration in vitro and promoted bone metastases in vivo. Furthermore, specific siRNA-induced knockdown of MGr1-Ag expression in SBC-5 cells suppressed the potential of cell invasion and migration in vitro and dramatically decreased the number and sites of bone metastasis in vivo. We also found that MGr1-Ag induced SCLC cells to undergo epithelial-mesenchymal transition (EMT), as demonstrated by cell morphological changes, decreased expression of epithelial markers and increased expression of mesenchymal markers. Taken together, we conclude that MGr1-Ag promotes SCLC cell invasion and bone metastasis in vitro and in vivo, and that this is partially mediated via the EMT pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang F, Wang Y, Xu M, Dong H, Liu N, Zhou J, Pang H, Ma N, Zhang N, Pei Y, Pei Y, et al: MGr1-Ag promotes invasion and bone metastasis of small-cell lung cancer in vitro and in vivo. Oncol Rep 29: 2283-2290, 2013.
APA
Zhang, F., Wang, Y., Xu, M., Dong, H., Liu, N., Zhou, J. ... Liu, L. (2013). MGr1-Ag promotes invasion and bone metastasis of small-cell lung cancer in vitro and in vivo. Oncology Reports, 29, 2283-2290. https://doi.org/10.3892/or.2013.2396
MLA
Zhang, F., Wang, Y., Xu, M., Dong, H., Liu, N., Zhou, J., Pang, H., Ma, N., Zhang, N., Pei, Y., Zhang, H., Liu, L."MGr1-Ag promotes invasion and bone metastasis of small-cell lung cancer in vitro and in vivo". Oncology Reports 29.6 (2013): 2283-2290.
Chicago
Zhang, F., Wang, Y., Xu, M., Dong, H., Liu, N., Zhou, J., Pang, H., Ma, N., Zhang, N., Pei, Y., Zhang, H., Liu, L."MGr1-Ag promotes invasion and bone metastasis of small-cell lung cancer in vitro and in vivo". Oncology Reports 29, no. 6 (2013): 2283-2290. https://doi.org/10.3892/or.2013.2396
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang F, Wang Y, Xu M, Dong H, Liu N, Zhou J, Pang H, Ma N, Zhang N, Pei Y, Pei Y, et al: MGr1-Ag promotes invasion and bone metastasis of small-cell lung cancer in vitro and in vivo. Oncol Rep 29: 2283-2290, 2013.
APA
Zhang, F., Wang, Y., Xu, M., Dong, H., Liu, N., Zhou, J. ... Liu, L. (2013). MGr1-Ag promotes invasion and bone metastasis of small-cell lung cancer in vitro and in vivo. Oncology Reports, 29, 2283-2290. https://doi.org/10.3892/or.2013.2396
MLA
Zhang, F., Wang, Y., Xu, M., Dong, H., Liu, N., Zhou, J., Pang, H., Ma, N., Zhang, N., Pei, Y., Zhang, H., Liu, L."MGr1-Ag promotes invasion and bone metastasis of small-cell lung cancer in vitro and in vivo". Oncology Reports 29.6 (2013): 2283-2290.
Chicago
Zhang, F., Wang, Y., Xu, M., Dong, H., Liu, N., Zhou, J., Pang, H., Ma, N., Zhang, N., Pei, Y., Zhang, H., Liu, L."MGr1-Ag promotes invasion and bone metastasis of small-cell lung cancer in vitro and in vivo". Oncology Reports 29, no. 6 (2013): 2283-2290. https://doi.org/10.3892/or.2013.2396
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