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Article

Anoxia/reoxygenation induces epithelial-mesenchymal transition in human colon cancer cell lines

  • Authors:
    • Manabu Okajima
    • Satoshi Kokura
    • Takeshi Ishikawa
    • Katsura Mizushima
    • Reiko Tsuchiya
    • Tatsuzo Matsuyama
    • Satoko Adachi
    • Tetsuya Okayama
    • Naoyuki Sakamoto
    • Kazuhiro Kamada
    • Kazuhiro Katada
    • Kazuhiko Uchiyama
    • Osamu Handa
    • Tomohisa Takagi
    • Nobuaki Yagi
    • Yuji Naito
    • Toshikazu Yoshikawa
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan, Department of Cancer Immuno Cell Regulation, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan, Department of Cancer Immuno Cell Regulation, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
  • Pages: 2311-2317
    |
    Published online on: April 10, 2013
       https://doi.org/10.3892/or.2013.2401
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Abstract

Epithelial-mesenchymal transition (EMT) is considered to be a crucial event in the development of cancer metastasis. Anoxia/reoxygenation (A/R) is known to occur in cancer tissues due to angiogenesis and changes in tissue pressure that occur during tumor growth. We investigated whether A/R induces EMT in the human colon cancer cell line HT-29. Colon cancer cells were exposed to anoxia (2 h) followed by reoxygenation (4-22 h) and evaluated for EMT changes using immunofluorescence and western blot analyses. We also investigated the expression of EMT-related transcription factors (Snail and ZEB1) using RT-PCR and evaluated the expression of NF-κB using ELISA. To determine whether NF-κB is involved in A/R-induced EMT, HT-29 cells were treated with proteasome inhibitors. Colon cancer cells exposed to A/R underwent EMT morphological changes; the cancer cells acquired a spindle-shaped phenotype. The expression of E-cadherin on the cell surface and the total amount of E-cadherin proteins were reduced after A/R. The expression of EMT-related transcription factors (Snail, ZEB1) was increased after A/R. Pretreatment with proteasome inhibitors significantly attenuated the downregulation of E-cadherin induced by A/R. These results indicate that A/R induces EMT in human colon cancer cells through an NF-κB-dependent transcriptional pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Okajima M, Kokura S, Ishikawa T, Mizushima K, Tsuchiya R, Matsuyama T, Adachi S, Okayama T, Sakamoto N, Kamada K, Kamada K, et al: Anoxia/reoxygenation induces epithelial-mesenchymal transition in human colon cancer cell lines. Oncol Rep 29: 2311-2317, 2013.
APA
Okajima, M., Kokura, S., Ishikawa, T., Mizushima, K., Tsuchiya, R., Matsuyama, T. ... Yoshikawa, T. (2013). Anoxia/reoxygenation induces epithelial-mesenchymal transition in human colon cancer cell lines. Oncology Reports, 29, 2311-2317. https://doi.org/10.3892/or.2013.2401
MLA
Okajima, M., Kokura, S., Ishikawa, T., Mizushima, K., Tsuchiya, R., Matsuyama, T., Adachi, S., Okayama, T., Sakamoto, N., Kamada, K., Katada, K., Uchiyama, K., Handa, O., Takagi, T., Yagi, N., Naito, Y., Yoshikawa, T."Anoxia/reoxygenation induces epithelial-mesenchymal transition in human colon cancer cell lines". Oncology Reports 29.6 (2013): 2311-2317.
Chicago
Okajima, M., Kokura, S., Ishikawa, T., Mizushima, K., Tsuchiya, R., Matsuyama, T., Adachi, S., Okayama, T., Sakamoto, N., Kamada, K., Katada, K., Uchiyama, K., Handa, O., Takagi, T., Yagi, N., Naito, Y., Yoshikawa, T."Anoxia/reoxygenation induces epithelial-mesenchymal transition in human colon cancer cell lines". Oncology Reports 29, no. 6 (2013): 2311-2317. https://doi.org/10.3892/or.2013.2401
Copy and paste a formatted citation
x
Spandidos Publications style
Okajima M, Kokura S, Ishikawa T, Mizushima K, Tsuchiya R, Matsuyama T, Adachi S, Okayama T, Sakamoto N, Kamada K, Kamada K, et al: Anoxia/reoxygenation induces epithelial-mesenchymal transition in human colon cancer cell lines. Oncol Rep 29: 2311-2317, 2013.
APA
Okajima, M., Kokura, S., Ishikawa, T., Mizushima, K., Tsuchiya, R., Matsuyama, T. ... Yoshikawa, T. (2013). Anoxia/reoxygenation induces epithelial-mesenchymal transition in human colon cancer cell lines. Oncology Reports, 29, 2311-2317. https://doi.org/10.3892/or.2013.2401
MLA
Okajima, M., Kokura, S., Ishikawa, T., Mizushima, K., Tsuchiya, R., Matsuyama, T., Adachi, S., Okayama, T., Sakamoto, N., Kamada, K., Katada, K., Uchiyama, K., Handa, O., Takagi, T., Yagi, N., Naito, Y., Yoshikawa, T."Anoxia/reoxygenation induces epithelial-mesenchymal transition in human colon cancer cell lines". Oncology Reports 29.6 (2013): 2311-2317.
Chicago
Okajima, M., Kokura, S., Ishikawa, T., Mizushima, K., Tsuchiya, R., Matsuyama, T., Adachi, S., Okayama, T., Sakamoto, N., Kamada, K., Katada, K., Uchiyama, K., Handa, O., Takagi, T., Yagi, N., Naito, Y., Yoshikawa, T."Anoxia/reoxygenation induces epithelial-mesenchymal transition in human colon cancer cell lines". Oncology Reports 29, no. 6 (2013): 2311-2317. https://doi.org/10.3892/or.2013.2401
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