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Article

Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines

  • Authors:
    • Tiesuo Zhao
    • Huijie Jia
    • Lifei Li
    • Guoliang Zhang
    • Min Zhao
    • Qian Cheng
    • Junnian Zheng
    • Di Li
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China, Laboratory of Transplantation and Immunology, Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China, Department of Respiration, Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028007, P.R. China, Department of Laboratory Medicine, Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028007, P.R. China, Department of Anatomy, The Medical College of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, P.R. China
  • Pages: 377-384
    |
    Published online on: April 18, 2013
       https://doi.org/10.3892/or.2013.2407
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Abstract

Lung cancer is a high-grade malignancy with poor 5 year-survival rates that remains incurable with current therapies. Different cellular stresses, including antitumor agents, ionizing radiation and ultraviolet (UV) light, can induce apoptosis and activate signaling pathways. UV has multiple effects on tumor cells, including DNA damage, and increases the expression of some genes involved in tumor cell apoptosis and DNA repair. It has been reported that UV can also activate casein kinase 2 (CK2). CK2, a Ser/Thr protein kinase, has been reported to be frequently overexpressed in various types of human cancer, including lung cancer, and is associated with tumor development. Thus, combination of UV and CK2 inhibitors may be a new strategy for the treatment of lung cancer. Our results demonstrated that inhibition of CK2a through CK2 siRNA or a CK2 inhibitor [(4,5,6,7-tetrabromobenzotriazole (TBB)] enhances the decrease in cell viability of lung cancer cells (A549 and H2030) induced by UV. Western blot analysis demonstrated that the combination increased the expression of apoptotic protein markers cytochrome c and the cleavage of poly ADP-ribose polymerase (PARP) and caspase-3. Furthermore, our results indicated that UV decreased the expression of the tumor suppressor protein PML through activation of CK2. Inhibition of CK2 by CK2 siRNA and TBB can recover the reduction of PML induced by UV. Collectively, these results demonstrate the significant apoptosis of lung cancer cells induced by combination treatment of the CK2 inhibitor and UV radiation. CK2 enhanced cell apoptosis by UV radiation may due, at least partly, to recover the expression of PML. These findings warrant the clinical testing of CK2 inhibitors which, when used in conjunction with DNA-damaging agents such as radiation, may be an effective cancer therapeutic strategy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao T, Jia H, Li L, Zhang G, Zhao M, Cheng Q, Zheng J and Li D: Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines. Oncol Rep 30: 377-384, 2013.
APA
Zhao, T., Jia, H., Li, L., Zhang, G., Zhao, M., Cheng, Q. ... Li, D. (2013). Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines. Oncology Reports, 30, 377-384. https://doi.org/10.3892/or.2013.2407
MLA
Zhao, T., Jia, H., Li, L., Zhang, G., Zhao, M., Cheng, Q., Zheng, J., Li, D."Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines". Oncology Reports 30.1 (2013): 377-384.
Chicago
Zhao, T., Jia, H., Li, L., Zhang, G., Zhao, M., Cheng, Q., Zheng, J., Li, D."Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines". Oncology Reports 30, no. 1 (2013): 377-384. https://doi.org/10.3892/or.2013.2407
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao T, Jia H, Li L, Zhang G, Zhao M, Cheng Q, Zheng J and Li D: Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines. Oncol Rep 30: 377-384, 2013.
APA
Zhao, T., Jia, H., Li, L., Zhang, G., Zhao, M., Cheng, Q. ... Li, D. (2013). Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines. Oncology Reports, 30, 377-384. https://doi.org/10.3892/or.2013.2407
MLA
Zhao, T., Jia, H., Li, L., Zhang, G., Zhao, M., Cheng, Q., Zheng, J., Li, D."Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines". Oncology Reports 30.1 (2013): 377-384.
Chicago
Zhao, T., Jia, H., Li, L., Zhang, G., Zhao, M., Cheng, Q., Zheng, J., Li, D."Inhibition of CK2 enhances UV-triggered apoptotic cell death in lung cancer cell lines". Oncology Reports 30, no. 1 (2013): 377-384. https://doi.org/10.3892/or.2013.2407
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